MICROBIOLOGY DISEASES BY TAX W/MAST MIC
5th Edition
ISBN: 9781323651674
Author: BAUMAN
Publisher: PEARSON C
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Chapter 8, Problem 6CT
Summary Introduction
To describe:
The number of
Introduction:
The
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During PCR, the reaction mixture cycles through three temperatures (for example 94, 60, and 72 degrees Celsius) multiple times. What happens during the 94 degree part of a cycle?
Group of answer choices
Double-stranded DNA denatures and becomes single-stranded.
DNA polymerase synthesizes daughter DNA molecules.
An oligonucleotide primer anneals to a template DNA strand.
PCR is a very useful method in biotechnology because it does not require the 6 or more different key proteins/enzymes required for DNA replication in living cells. Explain how and why this technique only requires 1 enzyme to make lots of DNA.
In both leading and lagging strand synthesis, DNA replication always proceeds in a certain direction. What direction is this?
Explain how oligonucleotide primers in the Polymerase Chain Reaction work (PCR)
Chapter 8 Solutions
MICROBIOLOGY DISEASES BY TAX W/MAST MIC
Ch. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MCCh. 8 - Which of the following would be most useful in...Ch. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MC
Ch. 8 - Prob. 1TFCh. 8 - Prob. 2TFCh. 8 - Prob. 3TFCh. 8 - ________ Protoplast fusion is often used in the...Ch. 8 - Prob. 5TFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - Prob. 2CTCh. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - How is the result of a Southern blot similar to...Ch. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - If a gene contains the sequence TACMTCGCATTGM,...Ch. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Why did the discovery and development of...Ch. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Why dont scientists who work with recombinant DNA...
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- Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerasearrow_forwardQuantitative PCR (qPCR) has one extra ingredient to quantify DNA. Describe its structure and explain how it changes during the reaction.arrow_forwardThe way PCR amplifies DNA is similar to the doubling in apopulation of growing bacteria—a single DNA strand is used tosynthesize 2 DNA strands, which become 4, then 8, then 16, etc. If acomplete cycle takes 3 minutes, how many strands of DNA wouldtheoretically be present after 10 minutes? After 1 hour?arrow_forward
- PCR (polymerase chain reaction) is an excellent method of generating copies of target DNA. If a single piece of double stranded DNA (dsDNA) is put into a PCR machine, how many dsDNA segments will there be after 3 rounds? A. 8 segments, with 2 original strands paired B. 16 segments, with 2 original strands on different segments C. 16 segments, with 2 original strands paired D. 8 segments, with 2 original strands on different segmentsarrow_forwardA. write one difference between DNA Replication in vivo and PCR. Draw two pictures which clearly illustrate that difference. (cannot say one occurs inside of cells and the other one does not).arrow_forwardThe figure below shows a molecule of DNA with its strands separated prior to DNA replication. The structure labeled “primer” on the figure below: A) will be used to construct the lagging strand B) is made of deoxyribonucleotides (DNA) C) reads 3’ to 5’ from left to right D) was made by DNA ligasearrow_forward
- What would happen if we use an endonuclease on DNA obtained from human cells(without PCR) & then electrophoresis it through a low-resolution gel.arrow_forwardWhich is the odd one out ? For the rest, explain the concept/process/technique they are involved with. DNA polymerase primers DNA RNA ddNTPsarrow_forwardPlease explain the reason To make a new DNA strand, you need (check ALL that apply)... a template strand heavy nitrogen nucleotidesarrow_forward
- When RNA polymerase transcribes DNA, only one of the two DNA strands is used as a template. Take a look and explain how RNA polymerase determines which DNA strand is the template strand.arrow_forwardGive only typing answer with explanation and conclusion A DNA ligase: None of the listed answers Can join two fragments of DNA binding the phosphodiester backbone with covalent bonds. All of the listed answers -not the correct answer Can recognize specific sequences that allow for the detection of SNPs. Can replicate a complementary DNA strand using a single stranded DNA template.arrow_forwardIn a PCR reaction, a few seconds at high temperature disrupts the ____ that hold the two strands of DNA together, so every molecule of DNA unwinds and becomes single-stranded. DNA ligases covalent bonds peptide bonds hydrogen bonds lipoproteinsarrow_forward
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