Biology: The Unity and Diversity of Life
15th Edition
ISBN: 9780357093795
Author: STARR
Publisher: CENGAGE LEARNING (CUSTOM)
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Textbook Question
Chapter 8, Problem 9SQ
Which of the following is/are not required for
- a. DNA polymerase
- b.
nucleotides - c. template DNA
- d. primers
- e. helicase
- f. all are required
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In the dideoxy-sequencing reaction, what terminates DNA synthesis at a particular base? a. The absence of a base on the ddNTP halts the DNA polymerase. b. The ddNTP causes a break in the sugar–phosphate backbone. c. DNA polymerase will not incorporate a ddNTP into the growing DNA strand. d. The absence of a 3′-OH group on the ddNTP prevents the addition of another nucleotide.
DNA polymerase III is a processive enzyme, which means that
a. it does not dissociate from the growing strand after it has attached a nucleotide to the 3′ end.
b. it makes a new strand very quickly.
c. it proceeds toward the opening of the replication fork.
d. it copies DNA with relatively few errors.
DNA polymerase requires the following for replication:
A.
Single stranded ("unzipped") DNA
B.
Double stranded ("zipped") DNA
C.
An RNA primer
D.
A and C
E.
B and C
Chapter 8 Solutions
Biology: The Unity and Diversity of Life
Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - HersheyChase Experiments The graph shown in FIGURE...Ch. 8 - Which is not a nucleotide base in DNA? a. adenine...Ch. 8 - What are the base-pairing rules for DNA? a. AG, TC...Ch. 8 - Similarities in ________ are the basis of...Ch. 8 - One species DNA differs from others in its _____....Ch. 8 - In eukaryotic chromosomes, DNA wraps around...
Ch. 8 - The chromosome number ______. a. refers to a...Ch. 8 - Prob. 7SQCh. 8 - When DNA replication begins, ______. a. the two...Ch. 8 - Which of the following is/are not required for DNA...Ch. 8 - Energy that drives the attachment of a nucleotide...Ch. 8 - The phrase "5 prime to 3 prime" refers to the...Ch. 8 - After DNA replication, a eukaryotic chromosome...Ch. 8 - Match the terms appropriately. ______...Ch. 8 - All mutations ________. a. result from radiation...Ch. 8 - Prob. 15SQCh. 8 - Determine the complementary strand of DNA that...Ch. 8 - Review Figures 8.12 and 8.13. In cells, the...Ch. 8 - Woolly mammoths have been extinct for about 4,000...Ch. 8 - Xeroderma pigmentosum is an inherited disorder...
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- DNA strands are anti-parallel and DNA polymerase can only synthesize DNA in a 5' to 3' direction. How does the enzyme synthesize both strands at the same time? A. The leading strand is sythesised in Okazaki fragments B. The lagging strand is synthesised in short Okazaki fragments. C. Only one strand is replicated. D. There are more than one DNA polymerase involved.arrow_forwardThe leading strand in DNA replication requires only one primer for its synthesis. The lagging strand requires several primers because a. the lagging strand is prepared in short segments b. the lagging strand lacks a topoisomerase c. the leading strand serves as a template for the lagging strand d. All DNA polymerases read from the 5′ end.arrow_forwardThe following diagram represents a DNA molecule that is undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forward
- In DNA replication, a primer is _____. a. what the original DNA strands are called b. a molecule that provides the energy for nucleotide attachments c. a regulatory protein that turns on the gene that starts DNA replication d. an enzyme that breaks the hydrogen bonds between base pairs e. a short piece of nucleic acid that serves as an attachment point for DNA polymerasearrow_forwardA.) Damaged DNA can be reversed if nucleotides can be replaced with a proper nucleotide for a correct amino acid base. B.) Direct reversal of DNA repair can destroy the abnormal bonds of the nucleotides in the DNA sequence. a. Statement A is correct b. Statement B is correct c. Both A and B are correct d. Both A and B are incorrectarrow_forwardIn order for DNA molecules to undergo recombination, ____. a. their strands must separate as in replication b. they must be from the same species c. one of the two DNA strands must be degraded d. they must be cut and spliced at specific nucleotide sequences e. they must first be transcribedarrow_forward
- What was the significance of Meselson and Stahl’s experiments on DNA replication using the heavy isotope of Nitrogen? A. telomerase was identified as the molecule responsible for solving the end replication problem of eukaryotic chromosomes B. the existence of lagging strand synthesis was proven. C. the rate of DNA synthesis by DNA polymerase was measured D. the processivity of DNA polymerase was established E. the semi-conservative mode of DNA replication was confirmedarrow_forwardPlace the steps of sanger sequencing in order.A. A fluorescent laser excites the fragments and records the wavelength consistent with a single nucleotide. B. ddNTPs bind and stop chain extension.C. DNA fragments are separated by size through a capillary tube. D. DNA polymerase copies the target region of template DNA.E. The final nucleotide of each fragment is labeled with a fluorescent tag.arrow_forwardWhich of the following is NOT true of DNA replication? a. Helicase uses ATP as an energy source. b. Primase uses ATP to build primers. c. Single-stranded binding proteins are enzymes that hold the strands open. d. Telomerase adds nucleotides to the original DNA template. e. Ligase uses a condensation reaction to make a single phosphodiester bond at each site of ligation..arrow_forward
- Polymerase Chain Reaction, or PCR, can Group of answer choices A. target a specific region of DNA and cut it out of the rest of the genetic material for further analysis. B. copy the number of copies of a selected region of DNA linearly. C. increase the number of copies of a selected region of DNA exponentially. D. copy the entire genome at least a dozen times.arrow_forwardBase analogs are mutagenic because of which characteristic? a. They produce changes in DNA polymerase that cause it to malfunction. b. They distort the structure of DNA. c. They are similar in structure to the normal bases. d. They chemically modify the normal bases.arrow_forwardThe following diagrams represent DNA molecules that are undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forward
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