GENETICS(LL)-W/CONNECT >CUSTOM<
6th Edition
ISBN: 9781260571561
Author: HARTWELL
Publisher: MCG CUSTOM
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Textbook Question
Chapter 9, Problem 12P
| a. | DNA polymerase |
| b. | RNA polymerase |
| c. | A restriction enzyme |
| d. | DNA ligase |
| e. | An aminoacyl-tRNA synthetase |
| f. | Peptidyl transferase |
| g. | Reverse transcriptase |
Which of the enzymes from the following list would you need to make a recombinant DNA molecule? What is the function of those enzyme(s) in the process?
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Match each enzyme name in the left column with the correct descriptive
phrase in the right column.
a. Topoisomerase II
b. DNA ligase
c. DNA polymerase y
d. Reverse transcriptase
i. Catalyzes most nucleotide incorporations
in bacterial DNA replication
ii. Cleaves RNA in a DNA-RNA hybrid
molecule
e. DNA polymerase I
f. DNA polymerase II
iii. Uses a tRNA primer in synthesis of
retroviral DNA
iv. Acts through an adenylylated DNA inter-
mediate
v. Catalyzes formation of a double-strand
DNA break
vi. Catalyzes mitochondrial DNA replication
Match the following polymerases with their function.
+ DNA Polymerase III
DNA polymerase I
+ RNA polymerase
+ RNA polymerase II
A. Polymerase responsible for proofreading
DNA
B. Polymerase responsible for making
prokaryotic mRNA
C. Polymerase responsible for making
eukaryotic MRNA
D. Polymerase responsible for making
prokaryotic DNA
Which enzyme is used in Sanger sequencing reactions?
O A. DNA polymerase
O B. S1 endonuclease
O C. ligase
O D. reverse transcriptase
O E. RNA-dependent DNA polymerase
Chapter 9 Solutions
GENETICS(LL)-W/CONNECT >CUSTOM<
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...
Ch. 9 - a. What is the purpose of molecular cloning? b....Ch. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - Is it possible that two different restriction...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Suppose you are using a plasmid cloning vector...Ch. 9 - Prob. 17PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - You use the primer 5 GCCTCGAATCGGGTACC 3 to...Ch. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - Problem 15 showed part of the sequence of the...Ch. 9 - Eukaryotic genomes are replete with repetitive...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- The enzyme responsible for the joining of Okazaki fragments is a. helicase b. primase c. DNA ligase d. DNA topoisomerasearrow_forwardThe general excision repair pathway for DNA repair has the following order A. endonuclease cuts → DNA ligase removes → endonuclease removes → DNA ligase B. DNA ligase → DNA polymerase → helicase or endonuclease removes → endonuclease cuts C. endonuclease cuts → helicase or endonuclease removes → DNA polymerase → DNA ligase D. endonuclease cuts → DNA polymerase repairs → helicase opens up → DNA ligasearrow_forwardWhich of the following statements is TRUE concerning the synthesis of the leading and lagging strands of DNA in prokaryotic cells? a. O b. The leading strand is synthesized by one polymerase III continuously, and the lagging strand is synthesized by several molecules of DNA polymerase III. d. The leading and lagging strands are synthesized at the same time by the one DNA polymerase I. O c. The leading and lagging strands are synthesized at the same time by the one DNA polymerase III. The leading strand is synthesized by one polymerase III, and the lagging strand is synthesized by DNA polymerase I.arrow_forward
- Enzyme function is critically important for the proper replication of DNA. Predict the consequence of a loss of function for each of the following enzymes. a. DNA gyrase b. DNA polymerase III c. DNA ligase d. DNA polymerase Iarrow_forwardMatch the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts as a template for DNA synthesis: RNA directs the cutting of an RNA molecule at a precise location: RNA directs the cutting of an DNA molecule at a precise location: options: a. Small Nuclear Ribonuclear Protein (SNRNP) b. telomerase c. primase d. helicase e. CRISPR/Cas9arrow_forwardThe short Okazaki fragments are Select one: a. spliced together by DNA ligase b. glued together by RNA primers c. fused together by DNA polymerase d. formed into the lagging strand without splicingarrow_forward
- Pyrimidine dimers in DNA may be repaired by in E. coli cells. (select all correct answers) Select one or more: O a. photolyase O b. nucleotide excision repair O c. mis-match repair O d. methyl-transferasearrow_forwardThe other options are: a. RNA cannot be digested by restriction enzymes b. RNA is small enough to be resolved on an agarose gel without the need for restriction digestion. c. RNA is single stranded and DNA is double strandedarrow_forwardA diagram of a prokaryotic replication fork is shown. As in class, RNA primers are represented by a wavy line and DNA represented by a straight line. From the list below pick all the statements that are TRUE of this replication fork. (Multiple answers possible) A.Primer sequences are not correct. B. DNA being synthesized is not complementary and antiparallel to the template. C. Direction of DNA synthesis is not correct (not 5' to 3'). D. At least one protein in the diagram is mislabeled.arrow_forward
- Describe the main jobs of each of the following enzymes in the E. coli model of replication: A. DNA Polymerase I B. DNA Polymerase II C. DNA Polymerase IIIarrow_forwardRestriction endonucleases are bacterial enzymes that cleave duplex (double-stranded) DNA at specific nucleotide sequences. The mode of replication of the animal virus SV40 has been investigated by using restriction endonucleases that cleave SV40 DNA into a number of unique segments. Like most viruses, SV40 DNA is circular. The map positions of the 11 fragments produced by a pair of restriction endonucleases are shown on the next page. Immediately following a 5 or 10 minute pulse of radioactively labeled thymidine, labeled SV40 molecules that have completed replication during the pulse are isolated. These newly replicated DNA molecules are digested by the restriction endonucleases and the resulting fragments are analyzed for the relative amounts of pulse label they contain. The results are in the table below. Assume that at the time the label was added there was a random population of replicating SV40 DNA molecules in all possible stages of synthesis. From the information given below,…arrow_forwardDefine the following terms: a. processivity b. replisome c. exonuclease d. DNA ligase e. repliconarrow_forward
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