ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
6th Edition
ISBN: 9781260406092
Author: HARTWELL, Leland, HOOD, Leroy, Goldberg, Michael
Publisher: Mcgraw-hill Education/stony Brook University
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Textbook Question
Chapter 9, Problem 21P
Which of the enzymes from the following list would you need to sequence DNA? What is the function of those enzyme(s) in the process?
a. | DNA polymerase |
b. | RNA polymerase |
c. | A restriction enzyme |
d. | DNA ligase |
e. | An aminoacyl-tRNA synthetase |
f. | Peptidyl transferase |
g. | Reverse transcriptase |
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Which of the following enzymes adds a new amino acid to the growing chain of a protein during protein synthesis?
a.
aminoacyl-tRNA synthetase
b.
peptidyl synthetase
c.
peptidyl transferase
d.
ribosomal synthetase
Restriction enzymes (type II) bind to their recognition site and
A. begin elongation from it
B. methylaet it
C. cut inside it
D. ligate it
Match the following polymerases with their function.
+ DNA Polymerase III
DNA polymerase I
+ RNA polymerase
+ RNA polymerase II
A. Polymerase responsible for proofreading
DNA
B. Polymerase responsible for making
prokaryotic mRNA
C. Polymerase responsible for making
eukaryotic MRNA
D. Polymerase responsible for making
prokaryotic DNA
Chapter 9 Solutions
ND STONY BROOK UNIVERSITY LOOSELEAF GENETICS: FROM GENES TO GENOMES
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The calculations of the average restriction...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Agarose gels with different average pore sizes are...Ch. 9 - The following picture shows the ethidium...Ch. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - The text stated that molecular biologists have...
Ch. 9 - a. What is the purpose of molecular cloning? b....Ch. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - Is it possible that two different restriction...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Suppose you are using a plasmid cloning vector...Ch. 9 - Prob. 17PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - You use the primer 5 GCCTCGAATCGGGTACC 3 to...Ch. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - Problem 15 showed part of the sequence of the...Ch. 9 - Eukaryotic genomes are replete with repetitive...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Which of the following sequences, when combined with itscomplement, would be clipped by a restriction endonuclease?a. ATCGATCGTAGCTA b. AAGCTTCGAA c. GAATTC d. ACCATTGGAarrow_forwardChoose the combination of answers that most accurately completes the statement.Which of the following sequences, when combined with its complement, would be clipped by a restriction endonuclease? a. ATCGATCGTAGCTA c. GAATTC b. AAGCTTCGAA d. ACCATTGGAarrow_forwardWhich of the following is synthesized from a DNA template? (Select one) a. Transducer RNA b. ribosomal RNA c. amino acids d. translation RNAarrow_forward
- Match the activity below with the correct enzyme. (You won't use all the enzymes listed.) RNA acts as a template for DNA synthesis: RNA directs the cutting of an RNA molecule at a precise location: RNA directs the cutting of an DNA molecule at a precise location: options: a. Small Nuclear Ribonuclear Protein (SNRNP) b. telomerase c. primase d. helicase e. CRISPR/Cas9arrow_forwardWhat is a restriction endonuclease? Select one: a. It is an enzyme that cleaves at a specific nucleotide sequence. b. It restricts the movement of the DNA outside the nucleus. c. It proofreads the DNA for accidental damages and corrects any errors. d. It is an enzyme that separates the DNA double helix.arrow_forwardCertain restriction endonucleases produce cohesive (sticky) ends. This means that they: a. stick tightly to the ends of the DNA they have cut. b. cut both DNA strands at the same base pair. c. make a staggered double-strand cut, leaving ends with a few nucleotides of single-stranded DNA protruding. d. cut in regions of high GC content, leaving ends that can form more hydrogen bonds than ends of high AT content. e. cut in regions of high AT content, leaving ends that can form more hydrogen bonds than ends of high GC content.arrow_forward
- Choose the one answer that fits best. Which statement regarding Molecular Biology is NOT correct (videos)? a. Taq Polymerase was isolated from an organism found in Yellowstone Park b. Restriction enzymes leave sticky ends c. DNA sequencing allows us to read DNA sequences d. Restriction enzymes cut DNA at specific sites e. EcoRI and HindII are commonly used polymerasesarrow_forwardA mutant DNA strand was transcribed then translated to proteins. a. What is the protein product of the mutant DNA strand? The sequence of the mutant strand is shown below: 5'-TGCCATAACTGTTCGTACTGGCAAATTGCC-3' 3'-ACGGTATTGACAAGCATGACCGTTTAACGG-5' b. The mutation altered the sequence of the wild type template DNA such that a degenerate codon for a basic amino acid in the wild type was converted to a non-degenerate codon resulting in the sequence for the mutant strand shown. What was the original amino acid? c. Compare the charges and pl of the mutant peptide and the normal (wild- type) peptide at physiological pH?arrow_forwardDefine the following terms: a. processivity b. replisome c. exonuclease d. DNA ligase e. repliconarrow_forward
- During proofreading, which of the following enzymes reads the DNA? a. primase b. topoisomerase c. DNA pol d. helicasearrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forwardYou are trying to clone a gene. You have successfully isolated it from the genomic DNA of an organism using the Hindlll restriction enzyme. You then take a plasmid with a single EcoRI restriction site and cleave it with EcoRI. You combine these two fragments and treat them with DNA ligase. Answer the two questions below. a.(2 points Does the cloning reaction succeed as described? If so, what is the product obtained? b. Explain your answer above.arrow_forward
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