Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 2R
Differentiate the following terms. Which one is “hit and miss”— that is, does not add a specific gene to a cell?
- a. protoplast fusion
- b. gene gun
- c. microinjection
- d. electroporatio
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Please answer questions 1.a, 1.b, and 1.c
Question 1.a
Which of the following is a limitation of RTV gene therapy?
a. It can only be used on somatic cells
b. The virus can repackage its RNA genome and cause widespread infection
c. Random insertion of DNA into the genome can affect existing necessary genes
d. The RNA genome cannot be copied into DNA
Question 1.b
Please choose the TRUE statement:
a. Somatic cell transformation is easier than germline cell transformation
b. Just like changes to the germline, changes in somatic cells are also heritable so these changes would pass onto the next generation of offspring
c. In AAV, transcription and translation of the dsDNA (therapeutic DNA) uses the host machinery
d. Someone with an autosomal dominant genetic disease can receive AAV or RTV as a cure
Question 1.c
Which of the following is the only gene therapy that does not introduce novel DNA into cells?
a. RTV gene therapy
b. AAV…
Information obtained by microscopic analysis of stained chromosomes is used in: a. radiation hybrid mapping b. sequence mapping c. RFLP mapping d. cytogenetic mapping
Choose the combination of answers that most accurately completes the statement.Which of the following characteristics is not true of a plasmid? a. It is a circular piece of DNA. b. It is required for normal cell function. c. It is found in bacteria. d. It can be transferred from cell to cell.
Chapter 9 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- which method in biotechnology is used to read and understand an oganisum DNAarrow_forwardIf improved diagnostic tests are developed from this project, is the effort worth it if the cancers diagnosed have no treatment available?arrow_forwardWhat are plasmids and vectors how are they used in cloning and research? Write the answer in following heading: What are plasmids ,What are vectors Plasmids in cloning and plasmids in research Vectors in cloning and vectors in research. Write an answer of about 2 to 3 pagesarrow_forward
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