Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 9, Problem 7MCQ
Pieces of human DNA stored in yeast cells.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
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When you performed the phage titer experiment you performed several dilutions of the original stock. Which plate was useful?
a. the dilution that produces the plate with no visible virus
b. the plate with fewer than 25 viral infections of E. coli
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The activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are
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b) dephosphorylated CpG islands.
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Chapter 9 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwardIn the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a countable plate b. to have duplicates to count c. As a control d. to be certain the E. coli was not previously infectedarrow_forwardWhich is the BEST technique to use to answer this question? You wish to determine whether a point mutation is present in the sunscreen gene. a. Westem blot. b. Northern blot . c. Sanger DNA sequencing d. In situ hybridization. Part B. Which is the BEST technique to use to answer this question? You wish to determine where Sunshine protein is translated in a ladybug embryo. a. Immunofluorescencez b. Western blot . c. RT-PCR. d. Northern blot. e. in situ hybridization.arrow_forward
- Common vectors used to transfer a piece of DNA into a cloning host are ________. A) artificial chromosomes B) bacteriophages C) All of the choices are correct D) viruses E) plasmidsarrow_forwardWhen E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as : A. Ligation. B. Transformation. C. Transfection. D. Digestion.arrow_forwardChoose the combination of answers that most accurately completes the statement. Which of the following is or are required for cloning a gene? a. restriction endonuclease d. a and b b. vector e. all of these c. cloning hostarrow_forward
- P-element transformation is a method that can be used to generate transgenic ______. A.) mice B.) bacteria C.) fruit flies D.) plantsarrow_forwardafter a successful infection by a viral particle, it integrates its genome into the host DNA. which term best describe the change of host genome? a. none b.silent mutation c.missense mutation d.frame shift mutationarrow_forwardYou have extracted a long piece of DNA from a human cell and you want to extract the gene of interest for you to clone it. Assuming that you know the sequence of the DNA what methods can youuse to amplify the gene of interest? A. antibody purification B. polymerase chain reaction C. none of the above D. ligationarrow_forward
- A. What is the pathogen that is attacking bananas today?b. Why is this especially problematic in Africa? C. Why do we expect to lose the war with this pathogen?d. What is random mutagenesis?arrow_forwardFollowing a CRISPR mediated DNA double-strand break, how can the DNA be repaired by the cell? Choose all that apply. A. Base pairing between complementary sticky sides. B. Nonhomologous end joining. C. Homology directed repair. D. Crossing over between nonhomologous chromosomes.arrow_forwardUsing the data in Table, identify restriction enzymes that (a) produce blunt ends; (b) recognize and cleave the same sequence (called isoschizomers); (c) produce identical sticky ends.arrow_forward
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