MICROBIOLOGY W/ACCESS PKG >IP<
12th Edition
ISBN: 9781323592427
Author: Tortora
Publisher: PEARSON C
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Textbook Question
Chapter 9, Problem 3MCQ
Which of the following is the fourth basic step to genetically modify a cell?
- a. transformation
- b. ligation
- c. plasmid cleavage
- d. restriction-enzyme digestion of gene
- e. isolation of gene
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When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
Ligation.
B.
Transformation.
C.
Transfection.
D.
Digestion.
The function of a restriction enzyme is to
a. prevent the movement of DNA outside the nucleus
b. separate the DNA double helix
c. cut the nucleotide sequence at a specific location in DNA
d. proofread DNA for accidental damages and corrects these errors
. Define the following terms:a. biotechnology: ____________________________________________________________b.genetic engineering: ________________________________________________________c. sticky ends: ______________________________________________________________d. plasmid: ________________________________________________________________e. recombinant DNA: _________________________________________________________f. genetic marker: ___________________________________________________________g. transgenic organism: _______________________________________________________h. clone: __________________________________________________________________i. vector: _________________________________________________________________
Chapter 9 Solutions
MICROBIOLOGY W/ACCESS PKG >IP<
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- Which gene is incorporated into plasmids to detect recombinantcells?a. restriction endonuclease b. virus receptors c. a gene for antibiotic resistance d. reverse transcriptasearrow_forwardThe activity of restriction enzymes may produce fragments with sticky ends. Sticky ends are a) a type of endonucleases. b) dephosphorylated CpG islands. c) unpaired nucleotides. d) double breaks with blunt ends.arrow_forwardWhich of the following characteristics is not true of a plasmid?a. It is a circular piece of DNA.b. It is required for normal cell function.c. It is found in bacteria.d. It can be transferred from cell to cell.arrow_forward
- Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardArrange the following steps in the sequence they would happen in a DNA cloning experiment. a. sealing DNA fragments into vectors with DNA ligase; b. utilizing a probe to detect a clone in the library; c. sequencing the clone's DNA; d. creating a DNA library of clones; e. cutting genomic DNA with restriction enzymes. A. e,a,d,b,c B. a,d,b,c,e C. c,b,e,a,d D. e,d,a,c,barrow_forwardWhat is the enzymatic function of restriction enzymes? Group of answer choices a. to cut nucleic acids at specific sites b. to join nucleotides during transcription c. to add new nucleotides to the growing strand of DNA d. to repair breaks in sugar - phosphate backbonesarrow_forward
- which of the following are considered as the main tools for dna technology? a. vectors b. plasmids c. organisms d. enzymesarrow_forwardWhich of the following is a function of a restriction endonuclease? a. It cleaves a DNA molecule at a specific site. b. It cleaves a DNA molecule at random locations. c. It removes specific codons from an mRNA molecule. d. It removes specific exons from an mRNA molecule.arrow_forwardWhich of the following objects would you use microinjection in order to transfer DNA into? a. Egg cell visible to the human eye b. Animal virions c. Escherichia coli that has an F plasmid d. Bacteria that has been heat shockedarrow_forward
- Restriction enzymes (type II) bind to their recognition site and A. begin elongation from it B. methylaet it C. cut inside it D. ligate itarrow_forwardWhich of the following statements applies to both point and frameshift mutations? A. A base is removed from a DNA template strand B. A base is added to a DNA template strand C. A base is substituted for another base in a DNA template strand D. No correct responsearrow_forwardDescribe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your description.arrow_forward
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