MICROBIOLOGY W/ACCESS PKG >IP<
12th Edition
ISBN: 9781323592427
Author: Tortora
Publisher: PEARSON C
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Textbook Question
Chapter 9, Problem 8MCQ
A population of cells carrying a desired plasmid.
- a. antisense
- b. clone
- c. library
- d. Southern blot
- e. vector
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When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :
A.
Ligation.
B.
Transformation.
C.
Transfection.
D.
Digestion.
You take an aliquot of competent cells and transform them with pUC plasmid. What should be the part of the selection medium? a) X Gal b) Ampicillin c) IPTGd) All should be part of the selection medium
When you performed the phage titer experiment you performed several dilutions of the original stock. Which plate was useful?
a. the dilution that produces the plate with no visible virus
b. the plate with fewer than 25 viral infections of E. coli
c. the plate with the most dense virus
d. the plate with between 30 and 300 visible viral infections of E. coli
Chapter 9 Solutions
MICROBIOLOGY W/ACCESS PKG >IP<
Ch. 9 - Compare and contrast the following terms: a. cDNA...Ch. 9 - Differentiate the following terms. Which one is...Ch. 9 - Some commonly used restriction enzymes are listed...Ch. 9 - Suppose you want multiple copies of a gene you...Ch. 9 - Which enzyme makes the smallest fragment...Ch. 9 - Describe a recombinant DNA experiment in two or...Ch. 9 - List at least two examples of the use of rDNA in...Ch. 9 - You are attempting to insert a gene for saltwater...Ch. 9 - How does RNAi silence a gene?Ch. 9 - Prob. 10R
Ch. 9 - Restriction enzymes were first discovered with the...Ch. 9 - The DNA probe, 3-GGCTTA, will hybridize with which...Ch. 9 - Which of the following is the fourth basic step to...Ch. 9 - The following enzymes are used to make cDNA. What...Ch. 9 - If you put a gene in a virus, the next step in...Ch. 9 - You have a small gene that you want replicated by...Ch. 9 - Pieces of human DNA stored in yeast cells. a....Ch. 9 - A population of cells carrying a desired plasmid....Ch. 9 - Self-replicating DNA for transmitting a gene from...Ch. 9 - A gene that hybridizes with mRNA. a. antisense b....Ch. 9 - Design an experiment using vaccinia virus to make...Ch. 9 - Why did the use of DNA polymerase from the...Ch. 9 - The following picture shows bacterial colonies...Ch. 9 - Prob. 1CAECh. 9 - Using the restriction enzyme ECORI, the following...
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- What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reactionarrow_forwarda) Where is the F plasmid located in an Hfr cell? Where is the F plasmid located in an F+ cell? b)arrow_forwardChoose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescentarrow_forward
- You want to propagate large amounts of a DNA fragment. To do this, use a plasmid vector. a) How should this plasmid vector be constructed? b) Also describe the function of the components.arrow_forwardWhich of the following may be used as a vector in a gene-cloningexperiment?a. mRNA c. Virusb. Plasmid d. Both b and carrow_forwardWhich of the following characteristics is not true of a plasmid?a. It is a circular piece of DNA.b. It is required for normal cell function.c. It is found in bacteria.d. It can be transferred from cell to cell.arrow_forward
- Explain how you would use the blue-white colony screening technique to identify bacteria containing the recombinant plasmidarrow_forwardIn the phage titer experiment, why did you plate multiple dilutions? a. so that you should have a countable plate b. to have duplicates to count c. As a control d. to be certain the E. coli was not previously infectedarrow_forwardCommon vectors used to transfer a piece of DNA into a cloning host are ________. A) artificial chromosomes B) bacteriophages C) All of the choices are correct D) viruses E) plasmidsarrow_forward
- Which of the statements is TRUE about conjugation? a) Plasmid DNA is transferred from a donor (F+) cell b) It may only be performed by competent cells c) DNA is always integrated into recipient chromosome d) It may be specialized or generalizedarrow_forwardA strain with the ability to conjugate is mixed with a recipient strain. Recipients are noted to become recombinant but F-. This indicates. a. unsuccessful completion of conjugation b. the donor strain has a defective plasmid c. the donor strain has a plasmid integrated in the chromosome d. the recipient has a defective recA genearrow_forwardwhich of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase d. a growth medium with carefully selected ingredients that take advantage of selectable markers. e. none of the above.arrow_forward
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