Genetics: From Genes to Genomes, 5th edition
5th Edition
ISBN: 9780073525310
Author: Leland H. Hartwell, Michael L. Goldberg, Janice A. Fischer, Leroy Hood, Charles F. Aquadro
Publisher: McGraw-Hill Education
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Chapter 9, Problem 4P
Why do longer DNA molecules move more slowly than shorter ones during electrophoresis?
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How does size of DNA affect size traveled during electrophoresis?
Explain why DNA fragments migrate in a gel electrophoresis. Which fragments migrate farthest: large or small?
Explain how agarose electrophoresis separates DNA fragments. Why does a smaller DNA fragment move
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Chapter 9 Solutions
Genetics: From Genes to Genomes, 5th edition
Ch. 9 - Match each of the terms in the left column to the...Ch. 9 - For each of the restriction enzymes listed below:...Ch. 9 - The DNA molecule whose entire sequence follows is...Ch. 9 - Why do longer DNA molecules move more slowly than...Ch. 9 - Prob. 5PCh. 9 - The linear bacteriophage genomic DNA has at each...Ch. 9 - Consider a partial restriction digestion, in which...Ch. 9 - Prob. 8PCh. 9 - a. DNA polymerase b. RNA polymerase c. A...Ch. 9 - A plasmid vector pBS281 is cleaved by the enzyme...
Ch. 9 - A recombinant DNA molecule is constructed using a...Ch. 9 - Prob. 12PCh. 9 - The lacZ gene from E. coli encodes the enzyme...Ch. 9 - Your undergraduate research advisor has assigned...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - Prob. 16PCh. 9 - Prob. 17PCh. 9 - a. To make a genomic library useful for sequencing...Ch. 9 - showed part of the sequence of the plasmid vector...Ch. 9 - List three independent techniques you could use to...Ch. 9 - Figure 9.11a on p. 318 has numbers indicating the...Ch. 9 - Which of the enzymes from the following list would...Ch. 9 - Prob. 23PCh. 9 - a. What sequence information about a gene is...Ch. 9 - Why do geneticists studying eukaryotic organisms...Ch. 9 - Prob. 26PCh. 9 - The human genome has been sequenced, but we still...Ch. 9 - This problem investigates issues encountered in...Ch. 9 - Give two different reasons for the much higher...Ch. 9 - Using a cDNA library, you isolated two different...Ch. 9 - Prob. 31PCh. 9 - An interesting phenomenon found in vertebrate DNA...Ch. 9 - Prob. 33PCh. 9 - Prob. 34PCh. 9 - In the human immune system, so-called B cells can...Ch. 9 - Chimpanzees have a set of hemoglobin genes very...Ch. 9 - Complete genome sequences indicate that the human...Ch. 9 - On your computers browser, view the page accessed...Ch. 9 - Prob. 39PCh. 9 - Certain individuals with mild forms of...Ch. 9 - The 1 and 2 genes in humans are identical in their...
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- During gel electrophoresis, DNA molecules can easily be separated according to size because all DNA molecules have the same charge-to-mass ratio and the same shape (long rod). Would you expect RNA molecules to behave in the same manner as DNA during gel electrophoresis? Why or why not?arrow_forwardGel electrophoresis drives DNA along an electrical current from a negative electrode to a positive electrode. This is because DNA is a negatively charged molecule. Do you think that gel electrophoresis would function differently if DNA was positively charged? How so? Why?arrow_forwardDuring dna electrophoresis what fragments migrate towards? Larve or small! Which charge slower than small fragments? Positive or negative?arrow_forward
- During agarose gel electrophoresis, why does DNA move through the gel when electric current is applied? because DNA is negatively charged because a charged chemical from the loading buffer is bound to the DNA because DNA is positively charged because DNA absorbs electricityarrow_forwardWould you expect RNA molecules to behave in the same manner as DNA during gel electrophoresis?arrow_forwardWhat is generated from the replication of DNA ? what method is used ? Describe the process. What are Okazaki fragments? What enzymes are used ?arrow_forward
- What do you mean by Electrophoresis?arrow_forwardHow can DNA fragments be separated by size?arrow_forwardDNA molecules of different sizes are often separated with the use of a technique called electrophoresis . With this technique, DNA molecules are placed in a gel, an electrical current is applied to the gel, and the DNA molecules migrate toward the positive (+) pole of the current. What aspect of its structure causes a DNA molecule to migrate toward the positive pole?arrow_forward
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardWhy is strand separation beneficial?arrow_forwardWhat increases transformation efficiency?arrow_forward
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