Essentials of Genetics
9th Edition
ISBN: 9781292108933
Author: KLUG
Publisher: PEARSON
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Chapter CHST3, Problem 2RQ
Summary Introduction
To review:
The reason behind using short tandem repeats (STRs) as a very common loci for
Introduction:
Microsatellites comprises a set of 2 to 13
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Why do you suppose that forensic DNA analysis relies principally onshort tandem repeats (repeat polymorphisms) rather than single-nucleotidepolymorphisms?
What are STRs, and why are they useful for DNA profiling?
What are three major limitations of forensic DNA profiling?
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- Whether done manually or automated, DNA sequencing gels are always made of polyacrylamide rather than agarose. Why can't agarose be used for a sequencing gel, as it is for other DNA gel electrophoresis?arrow_forwardWhy is it important that the alcohol used in the DNA extraction is kept cold?arrow_forwardWhy are entire genomes not used for DNA profiling?arrow_forward
- What is the role of EDTA in the DNA isolation procedure?arrow_forwardwhat are the advantages and disadvantages of Gel electrophoresis in DNA analysisarrow_forwardThe last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?arrow_forward
- What is the sequence of the sample DNA submitted for sequencing given the gel electrophoresis profile from the Sanger sequencing method?arrow_forwardCan you please interpret and discuss and mark, this gel electrophoresis results. DNA ladder sizes: Band1: 23130, Band2: 9416, Band3: 6557, Band4: 4361, Band5: 2322, Band6: 564, Band7: 125arrow_forwardWhich of the following pairs of sequences would be considered different alleles in DNA profiling? a) ATGAATTCGG; ATGAAATCGG b) ATGAATTCGG; TACTTACTTACT c) GAAGAAGAA; GAAGAAGAAGAA d) AATAATAATAAT; AATTAATTAATTarrow_forward
- The following question is related to Restriction Enzymes and RFLP. Using EcoRl, the smallest fragments between DNA sequence A and B were how many base pairs?arrow_forwardA DNA fragment with 450 bp will be closer to the top (negative pole) of an electrophoresis gel than one with 2,500 bp.True or false?arrow_forwardIf insert DNA was obtained by PCR using Taq DNA polymerase, what shape does the end of this DNA fragment have?arrow_forward
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