EBK ESSENTIALS OF GENETICS
9th Edition
ISBN: 8220102741614
Author: Palladino
Publisher: YUZU
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Chapter CHST3, Problem 4DQ
The phenomena of somatic mosaicism and chimerism are more prevalent than most people realize. For example, pregnancy and bone marrow transplantation may lead to a person's genome becoming a mixture of two different genomes. Describe how DNA
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Do you think DNA should be collected and genetic information entered into CODIS under any circumstances? If yes, do you think DNA should be collected from persons who are merely arrested or should a person be convicted of a crime before DNA can be collected? Would any crime qualify for DNA collection or just certain crimes? Which crimes? How long should CODIS retain that information? Who should be able to access that information and under what circumstances?
What does the symbol “N” indicate (see the arrow)? Is this a problem for getting an accurate DNA sequence?
The height of the peak in the figure is an indication of the quality of the data, with a high peak correlated with a low error in “base-calling” of perhaps 1 error in 10,000 bases. Is this generally a high-quality DNA sequence for determining the true DNA sequence of the gene studied? How can you tell?
Which end of the sequence shown above is the 5’ end of the sequence being made via PCR.
Based from Fig. 1B, which of the following lanes contain a good quality genomic DNA? Bad quality genomic DNA? Why or Why not? Answer in maximum of 5 sentences only. You can prove your answer by describing the banding pattern of each lanes and how to troubleshoot the DNA extraction procedure if possible.
Chapter CHST3 Solutions
EBK ESSENTIALS OF GENETICS
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- DNA fingerprinting and Restriction Fragment Length Polymorphism (RFLP) analysis are often used to test for paternity. A child inherits chromosomes from both mother and father, so DNA from a child displays restriction fragments derived from each parent. In the gel shown below, which child, if any, can be excluded as being the biological offspring of the putative father? Lane M is the sample from the mother, F from the putative father, and C1, C2, and C3 from the childrenarrow_forwardWhat are some difficulties with low copy number DNA analysis? What kinds of checks and balances can a laboratory employ to ensure reliability of DNA profiles coming from low amounts of DNA templatearrow_forwardWhy is it important that the alcohol used in the DNA extraction is kept cold? The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA at the interface between the aqueous and alcohol layers. The solubility of any compound is reduced at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased precipitation of DNA in the aqueous layer. The solubility of any compound is increased at lower temperatures. The colder the temperature of the alcohol, the greater the yield of DNA due to increased solubility of DNA in the aqueous layer.arrow_forward
- Besides the examples listed in Table 24.3, list five types ofshort sequences that a geneticist might want to locate withina DNA sequence.arrow_forwardIn isolating DNA in strawberries, can you isolate other biomolecules aside from DNA just by using commonly available household materials and chemicals? Elaborate on your answer.arrow_forwardThe region of the normal hemoglobin gene used for genetic testing for sickle cell anemia contains a restriction site such that homozygous normal individuals show two DNA fragments. If a single nucleotide change in hemoglobin destroys that restriction site, then how many DNA fragments will be visible on a gel from individuals that are homozygous mutant? What about heterozygotes?arrow_forward
- How is the color of the spot coverted into useful data if data is collected from a Vicrochip DNA microarray that's from the colors of spots that illuminate when the spots have a laser shine on them? From the following which one is the best option The color of the spot is converted to a number that represents the intensity of green or red, so that the numerical intensity values can be compared between spots by a computer program The color of the spot is bright so that it can be interpreted visually by trained scientists The color of the spot is present on the chip, counted and a ratio of red-yellow and green-yellow is calculated which can be done by hand The color of the spot can't be converted None of the abovearrow_forwardWhich of the folllowing is the term used for ultra compact form of DNA coiling? a. supercoiling b.metacoiling c. ultracoiling d. telephone coilingarrow_forwardGive typing answer with explanation and conclusion to all parts Maxim-Gilbert and Sanger Sequencing are two different methods used to sequence DNA. Describe the general techniques of Maxim-Gilbert and Sanger DNA Sequencing. List the advantages and disadvantages of each.arrow_forward
- The sum of the lengths of DNA fragments 1-3 equal the total original length? True / Falsearrow_forwardThe last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?arrow_forwardWhat information can be gathered by using DNA gel electrophoresis? Describe the steps in the process and explain how and why gel electrophoresis can be used to separate DNA fragments.arrow_forward
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