Luciferase

Luciferins and Luciferase differ chemically in different organisms but they all require molecular energy (ATP) for the reaction. 2) The chemical energy in stage one excites a specific molecule (The Luminescent Molecule: the combining of Luciferase and Luciferin). The excitement is caused by the increased energy level of the luminescent molecule. The result of this excitement is decay

Bioluminescence and the Exploration of the Deep Sea Bioluminescence is a topic most people do not know about. When asked what bioluminescence is, that person probably could not answer the question. It is actually the chemical process that certain organisms are able to use in order to synthesize and emit light, but most people just identify these organisms as glowing animals, glowing plants, and glowing organisms. Most people have seen these glowing animals in action under the sea, considering

Yahya Bello Chemokine receptor CXCR3 mediates gene transcription Introduction The invasion of microorganisms constantly threatened many organisms and it has evolved systems of immune defense for the elimination of pathogens in the body (Shizuo Akira1, 2006). Innate and adaptive immunity are two branches that are comprised in the immune system (Shizuo Akira1, 2006). The innate immune system is the first line of the defense against pathogens and it is mediated by phagocytes, which includes macrophages

Bioluminescence and the Exploration of the Deep Sea Bioluminescence is a topic most people do not know about. It is actually the chemical process that certain organisms are able to use in order to synthesize and emit light. Most organisms that use bioluminescence live in the depths of the sea. The idea that natural light could be emitted from a living organism fascinated those throughout the field of science. When it was first discovered, bioluminescence impacted the way people thought and it

horizontal gel electrophoresis platform and covered with pH > 12 alkaline solution for 20 min without electrophoresis[15]. The slide were then neutralized and stained with 50 µl of 20 µg /ml ethidium promide. This cytotoxicity method of cell growth following to pro-mutagen exposure was detected under cytocharasin B and frequency binuclear cells. Fairbrain and co-workers reported the advantage of this technique include: i) high sensitivity for detecting low levels of DNA damage; ii) ability to detect

Tutorial Assignment 1 1. The title of the article is “ A Functional SNP in BNC2 Is Associated with Adolescent Idiopathic Scoliosis” Journal Reference: Ogura Y, Kou I, Miura S, et al. A functional SNP in BNC2 is associated with adolescent idiopathic scoliosis. The American Journal of Human Genetics. 2015; 97(2): 337-342. doi: 10.1016/j.ajhg.2015.06.012 2. The authors studied this subject as it relates to a very prevalent medical diagnosis around the world, Adolescent idiopathic Scoliosis (AIS)

human APML cell line, HL60 with a lentiviral vector carrying the luciferase gene. The luciferase-producing U937 and HL60 cells were injected via the tail-vein into NOD.Cg-Rag1 Il2rg/SzJ (NRG) mice. After 3-5 days, engraftment of AML cells was confirmed by bioluminescence imaging documenting one log increase in quantitative signal measured by the IVIS Xenogen reader. Pilot results in two animals demonstrate that both U937 and HL60 luciferase-producing cells successfully established leukemia (Fig 6). Thus

time point by defining the luciferase activities in cell extracts. Moreover, the use of a live cell luciferase substrate makes it possible to monitor changes in autophagic activity in live cells in real time. This method has been successfully used to identify positive and negative regulators of autophagy from cells treated with microRNA, siRNA and small molecule libraries.242- Farkas T, Hoyer-Hansen M, Jaattela M. Identification of novel autophagy regulators by a luciferase-based assay for the kinetics

Wnt3 plays an important role in the regulation of the adult hippocampus neurogenesis through activation of the canonical Wnt pathway. We found BPA decreased the Wnt3 expression and protein levels, leading to altered neurogenesis by BPA. We therefore investigated the specific involvement of the Wnt/β-catenin that altered due to exposure of BPA and reduced neurogenesis. NSCs culture were knockdown by Wnt3a siRNA followed by presence and absence of BPA treatment. We found that number of β-tubulin+ cells

Scientific Advancements Ever since the discovery of this natural phenomenon, bioluminescence has allowed scientists to make significant advancements that benefit the entire world. Significance of Research Bioluminescence is a “cold light”, meaning that the light source does not waste energy producing heat, making it highly effective. Further studies and exploration have allowed for major scientific advancements to take place. For instance, by splicing together firefly and marine bacteria