Luciferase

Introduction In this experiment, the enzyme Luciferase (Luc) was purified using differential centrifugation, precipitation, and a simplified chromatography procedure, followed by an enzyme activity assay using the enzyme’s natural substrate, Luciferin, to confirm the presence of Luc. Luciferase is a 61 kD enzyme naturally found in the tails of fireflies, a member of the Lampyridae family. Luc acts as a catalyst in an oxidation-reduction reaction with Luciferin, which allows these insects to exhibit

proteins that activate or inhibit the conversion of a substrate to a product. Often, enzymes catalyze reactions that are crucial for biological processes, but a few regulate other aspects of life, such as communication between a species. The enzyme luciferase catalyzes the reaction that allows fireflies to communicate with each other via emission of a yellow-green to yellow-orange colored light (Nakatsu, T. et al., 2006, 372). This reaction is a bioluminescence reaction, where chemical energy

Luciferase assay. N2a cells were seeded into 24-well plates and transfected with 0.2 g of 8XTOPFLASH reporter and 0.05 g of pRL-TK, 0.4 g WT-DISC1 or DN-DISC1 and 0.4 g mouse DLX2 [50] using polyethylenimine. 24 hours after transfection, TCF reporter activity was measured using the Dual-Luciferase Assay System (Promega). Behavioral tests The pregnant female mice were fed with Dox containing food (Bio-Serv, 200 mg/kg) from the beginning of pregnancy on embryonic day 0(E0). The Dox food was removed

known as bioluminescence. Luciferin and Luciferase Bioluminescence has been utilized by a variety of organisms for thousands of years. Within this time period, bioluminescence has been through a series of evolution making it unique to the organism that utilize them. Although the specific biochemical components and pathways tend to be varied among species, most bioluminescent processes involves a substrate and an enzyme known as luciferin and luciferase. The substrate Luciferin is a compound that

which will produce the final product- luciferase enzyme. To produce

and unique specie as it makes itself glow, mainly to attract a mate. Bioluminescence is a fascinating process by which organisms convert chemical energy to cold light. Inside the firefly’s lantern are the chemicals – luciferin and luciferase. When luciferin and luciferase are mixed together in the presence of oxygen and ATP, the fuel for the cell, the chemical reaction gives off energy in the form of light. Bioluminescence chemical reactions use natural resources that are easily replenished and are

resulted in 50% reduction of CPE or luciferase activity. Fig. 5. Modeling antiviral compound screening using the HuN4-secNluc virus. (A) Screening of antiviral siRNAs using HuN4-secNluc. siRNAs targeting the PRRSV Nsp9, ORF5, and ORF7 genes were used as model antiviral compounds. A scrambled siRNA (siScr) was used as the negative control. Marc-145 cells were transfected with the siRNAs at a final concentration of 100nM and then infected with HuN4-secNluc. The luciferase activity was measured at 48 h post-infection

Yahya Bello To test whether chemokine receptor CXCR3 mediates gene transcription Introduction SAA (Serum Amyloid A) function is similar to a C- reactive protein (an annular pentameric protein that is mostly found in blood plasma and its levels rise in response to inflammation. It considered as an acute-phase protein of hepatic origin, which increases following interleukin-6 secretion by macrophages and T cells) but acts as an acute phase protein, which can be used as an indicative, predictive or

Michelle Coyle 1321264 Bio 2C03 Dr. Dej/Gupta October 5th, 2015 Bio 2C03 Tutorial Assignment 1 1. 1 The title of the research paper that the article on Science Daily was referring to is A Functional SNP in BNC2 Is Associated with Adolescent Idiopathic Scoliosis. Full Citation: Ogura Y, Kou I, Miura S et al. A Functional SNP in BNC2 Is Associated with Adolescent Idiopathic Scoliosis. The American Journal of Human Genetics. 2015; 97(2): 337-342. doi:10.1016/j.ajhg.2015.06.012. 2. The authors decided

allow cloning upstream of Firefly luciferase (Method xxxx). 3.2.2. Sequential digest of SacI and BglII As there is no buffer in which both SacI and BglII exhibit >50% activity, a sequential digest was necessary (Method 2.3.). The digest was successful and the resulting cells were then lysed. 3.2.3. Transformation using DH5-alpha cells Transformation was carried as instructed in Methods2.4. The cloned VDR was placed in front of the Firefly and Renilla luciferases. A microplate was set up for the