Luciferase

I am a young researcher from India aspiring for a PhD position. I obtained my master degree in biochemistry from Osmania University, India. Currently I’m a senior research fellow in the laboratory of DNA Replication and Cell Cycle at National Institute of Immunology, India, under the supervision of Dr. Sandeep Saxena. The main focus of the lab is to study the role of non-coding RNAs in regulation of Cell Cycle and DNA replication and also to provide mechanical insights in understanding the checkpoint

Just finished discussing and letting the statistic guy checking our histogram. Please see the powerpoint and the excel file for the histogram. Yes, we do have a bimodal distribution, which I think it could be the mechanism of the cytoskeleton in responding to the mechanical strain -- during the small strain the cytoskeletons could respond to the change, however, at the high strain the cytoskeleton gradually improves their structures by aligning themselves in more towards one preferable direction

Edwanny Nivar Professor Fair Microbial Physiology and Genetics 12 November 2017 Role of phenol-soluble modulins in formation of Staphylococcus aureus biofilms in synovial fluid ABSTRACT/OVERVIEW QUESTIONS: 1. Who did the research, and where? a. This research was done by Sana S. Dastgheyb, Amer E. Villaruz, Katherine Y. Le, Vee Y. Tan, Anthony C. Duong, Som S. Chatterjee, Gordon Y. C. Cheung, Hwang-Soo Joo, Noree J. Hickok and Michael Otto. b. It was conducted at the Pathogen Molecular Genetics

3.1. MiR-206 was prominently downregulated in TNBC tissues and inversely correlated with VEGF Quantitative RT-PCR results show that expression levels of miR-206 are obviously lower in TNBC cell lines than those in non-TNBC cell lines (Fig. 1A). Similarly, TNBC tissues express prominently lower levels of miR-206 compared to non-TNBC tissue samples and normal breast tissues (Fig. 1B). It is worth noting that non-TNBC tissues expressed lower miR-206 compared to normal breast tissues but miR-206

Direct effects of Estrogenic Chemicals on Human Prostate Stem and Progenitor Cells To directly assess the actions of estrogens on prostate stem and progenitor cells and to evaluate the relevance of these findings in the human prostate gland, we recently derived in vitro and in vivo systems utilizing primary cells cultured from prostates of young, disease-free organ donors. Adult prostate stem cells were enriched by FACS (CD49fhi, Trop2hi) or 3-D matrigel culture to form prostaspheres (PS). In the

negatively charged plasmid DNA (pDNA) to form copolymer/ pDNA polyplexes, which is an important process to prevent pDNA degradation by nucleases also PDMAEMA-PHB-PDMAEMA/DNA polyplexes displayed significantly better gene transfection when using with luciferase/plasmid in Cos7 and HEK293

The process of aging is one many dread and try to avoid. Arguably even worse than aging are age related diseases that see their onset as people get older. Aging and metabolism have been found to be closely connected. As individuals age, they usually gain weight because metabolism slows and their body composition changes. According to a paper published in Nature, after age 45 the average person loses about 10% of their muscle mass each decade and that mass is generally just turned into fat because

The original goal of this study was to identify miRNAs that are differentially expressed in the brains of HIV-infected patients with HIV-associated neurocognitive disorders (HAND). These disorders characterize a spectrum neurological syndrome that affects up to one-fourth of all HIV/AID-infected humans. Three proposed mechanism contribute to HAND, those given were genetic host susceptibility factors, viral properties, and altered host immune responses. The first factor listed (altered host expressions)

RESULTS Airway epithelial cell PPARγ knockdown aggravates AAD induced lung resistance and inflammatory cell infiltration. Previously we have shown that PPAR gamma activation in lung ……. To test whether airway epithelial cell PPARγ knockdown exaggerates AAD induced airway remodeling and inflammation, we have developed airway epithelial cell specific PPARγ knockout mice (AEC-PPARγ-/-) and produced AAD with OVA sensitization and challenge as described previously (PMID: 22617759 and PMID: 23913958).

Part I Many neuromuscular disorders, such as facioscapulohumeral muscular dystrophy (FSHD) and myotonic dystrophy type 1 (DM1), are inherited in a dominant manner; therefore, therapies with the potential to silence disease-causing genes are important to investigate. With the emergence of candidate genes involved in various forms of myopathy, a safe and efficacious RNAi strategy can be modified to have broad-reaching effects in the treatment of these dieases.1 In order to ameliorate a myopathy, the