week 2 lab

LABORATORY REPORT: Aseptic Technique & Inoculation of Bacteria Guide Questions. 1. Why is direct flaming preferred when disinfecting loops and needles? 2. Why is it important to flame the entirety of the loop and not just the tip? What consequences can be seen when this process is not done correctly? 3. What is the difference between quadrant streak method A from method B? 4. Why do we use a pencil instead of a pen when labelling culture tubes or plates? Are there other alternatives in labelling? 5. Why is an inoculating needle used in Subculturing Techniques? Can a loop be used instead? NOTE: Please try to answer all of the question asked, i promised to give you a good ratings

Case Study #6: A 20 year old male college student develops a fever after gall bladder surgery and has a pus exudate around the incision. You receive a cotton swab of the exudate which you streak on a blood agar plate. Bacterial colonies on the plate show B hemolysis, and microscopic examination reveals Gram + cocci occurring singly, in pairs, and in short chains. Further testing reveals that the isolate is catalase negative and phenol red fermentation broth with mannitol turns yellow. 1. What is the name of the most likely bacterium causing this infection? 2. What is the most likely place that this bacterium originated to cause this infection?

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Question:- How to perform a bactericidal test bactericidal test of Dental Pulp Stem Cell (DPSC)?

Need help with questions 1) Evaluate the data for each organism. Is each organism equally impacted by the chemotherapeutic agents listed? Use your data to support your answer. 2) When you compare the data for the Gram-positive cell to the data for the Gram-negative cell, can you say there is similar impact? Use your data to support your answer. 3) Which antibiotic(s) is/are more effective against the Gram-positive cell type? Gram-negative cell type? Use your data to support your answers. 4) Which antibiotic(s) is/are least effective against the Gram-positive cell type? Gram-negative cell type? Use your data to support your answers.

Microbiology Unit 5

Q4. Why are some plates placed into a candle jar? Q6. What is the purpose of each kind of medium?

Please help! Im confused

What type

Q24:  A 7 dilution is performed on a culture of bacteria in order to perform viable plate counts. From the dilution, *0.1 mL* of solution is plated on solid media, and 31 colonies of bacteria grow on the petri dish.  Assuming each colony came from a single bacterium, how many bacteria are in a single mL of the original culture? Express your answer to two decimal places using standard notation. Since only 0.1 mL is put on the plate, this counts as an extra dilution!!! Any time less than 1 mL is transfered, a dilution is being performed. Any time more than 1 mL is transfered, a concentration is being performed.

Title: Spoilage of coconut water Aim:  To assess the diversity of psychrotrophic bacteria causing spoilage in coconut water. How might incubation of plates at 37°C influence the results of the quantitative dilution and plating of the coconut water samples?

bio 2 from Lab Manual 4 TH edition Leboffe and Pierce

Q7. Several bacteria were isolated from patients in a hospital. The bacteria were tested by the clinical lab team at the hospital and produced the following results. Isolate ID Gram stain result Catalase result StaphTex Blue result Q Gram positive cocci POSITIVE NEGATIVE R Gram positive cocci NEGATIVE POSITIVE S Gram negative rods POSITIVE POSITIVE T Gram positive rods NEGATIVE NEGATIVE U Gram positive cocci POSITIVE POSITIVE Which of the clinical isolates were likely S. aureus Which of the 3 cultures, A, B, or C, contains S. aureus? Justify your answer completely.

Microbiology question. Can you please choose the write answer and explain why it’s right and others are wrong.

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Asap please with unique answer

Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

Helping tags: Biology, bacteria, lag phase WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Thank you. TOPIC: Bacterial Growth Curve 1. How may the following growth and culture conditions affect the length of the lag phase? EXPLAIN YOUR ANSWERS. a) inoculum is from an old culture b) shifting cells from rich culture medium to a poorer one c) exponentially growing culture is transferred into the same medium under the same growth conditions

Microbiology question. Chose the right answer

Microbiology question. Chose the right answer

microbio q8

help on question 4

help me already

PLEASE ANS ASAP 1. When placed into a hypertonic solution, a bacterial cell will: a:die as a result of dessication. b: lyse c: swell d. take in more water than it releases .   2. Which one of the following methods of antimicrobial susceptibility testing requires a dilution series of the antibiotic and can provide a minimum inhibitory concentration? a. Gradient diffusion method b. Agar macrodilution method c. Broth microdilution method d. Disk diffusion method   3. Blood for culture is usually obtained from a vein located in which area?  a. Brachial b. Femoral c. Axillary d. Antecubital   4. All clinical specimens submitted to the clinical microbiology laboratory must be: a. all of these b. property and carefully collected. c. properly labeled. d. properly transported to the laboratory   5. Contact precautions are required for patients with a. all of these b. respiratory syncytial virus  c. Clostridium difficile c. associated diseases. d. infections caused by multidrug-resistant…

Answer question 3

Sample Station 7 A. If a student performs a Gram stain on a Gram negative bacterium and stops the stain technique after the iodine step and rinse, what color would you predict the cells to be if you looked at it right now under the microscope?