lab - late

INSTRUCTION: Give the principle or significance of the following practices:   1. Flaming the mouth or lid of culture tubes or plates after opening and before closing them. 2. Flaming the inoculating needle or loop before and after inoculation. 3. Holding caps, lids, or cotton plugs rather than putting them on the table while inoculating.

Instruction: answers must be in numbers. You are culturing bacteria using a petri dish, the bacteria grow very well on this plate you have. However, your microbiology instructor wants to know how many bacteria per milliliter (bacteria/mL) are on your plate. And as you have remembered during the first day of inoculating this bacteria you used a imL aliquots sample of a 1:10,000 dilution, and the total colony you counted on this plate is 170. How many bacterial per milliliters would that be?

List each step of learning goals that was listed from the picture

Direction: Read and analyze the following laboratory experiment and answer the following question. PART 1: SURFACE AREA AND CELL SIZE Materials: Agar containing NaOH, and the pH-indicator dye phenolphthalein cured into cubes of various size, 3 plastic cups, HCl, metric ruler, paper towels. Methodology: 1. Safety: Wear goggles and nitrile gloves while completing this lab. 2. Obtain three different size blocks of pink or blue agar. Using a ruler, measure the length, width, and height of the three blocks given below. Cut the agar according to the given dimension. Small = 1 cm x 1 cm x 1 cm Medium = 2 cm x 2 cm x 2 cm • • Large = 1 cm x 1 cm x 6 cm 3. Record your data. 4. Pour HCl or vinegar into two small cups. Place the one larger "cell" into one cup and the two smaller cells in the other cup. Start timing 30 minutes. 5. After 30 minutes, remove the cells and blot them dry with a paper towel. 6. Using your ruler, measure the distance the HCl has diffused into the blocks as shown on the…

Question:- Describe control strains used in the clinical microbiology laboratory and explain their maintenance in the laboratory. ( write BY WORD and all steps I need). Introduction Discussion References

Activity 2. Media Used in Isolating Coliforms You are a group of microbiologists tasked to test the presence of coliforms in the newly built water station. You are asked to isolate E. coli, Salmonella, and Shigella. Enumerate the media used for each step of isolating these bacteria. Include pictures and references. Bacteria Enrichment (Broth/Agar) Presumptive test (Broth/Agar) Isolation Media (Broth/Agar) E. coli Salmonella Shigella 1. How can you confirm that E. coli, Salmonella, and Shigella are present in the newly built water station? Explain. 2. What is the difference between nutrient broth and nutrient agar? 3. What is the importance of these steps (Enrichment, Isolation, and presumptive test) in isolating bacteria? Conclusion about the process of isolating bacteria

Subject : Environmental Microbiology  Can u use the information given below to answer these 2 question  1.Provide an aim for this lab 2. Provide objectives  DISCUSSION QUESTIONS   What is the relationship between the resolution power and the useful magnification that may be obtained with the light microscope?  What determines the resolving power of the lens system?                                What is the limit of resolution obtainable with the light microscope?                                                 How you will distinguish between bright field and dark-field microscopy and provide a specific example where each would be method of choice for observing a culture of bacteria?    What advantages does electron microscopy have over light microscopy?    What are disadvantages of electron microscopy over light microscopy?                               #Compare the use and the methodology of TEM with SEM? Provide at least one example where each would be the method of choic

Differential staining procedures gray stain and acid-fast stain lab report.

TITLE : INTRODUCTION TO BASIC BIOCHEMISTRY LABORATORY TECHNIQUES Objectives: Introducing to the students the typical setup of a biochemistry laboratory, safety requirements basic biochemistry laboratory skills. Significant figures. Biochemistry Laboratory practices is very important to understand the concepts practically. The qualitative and quantitative measures of any sample is done by biochemistry lab practices. Discuss about introduction to basic biochemistry laboratory techniques.

MATCHING TYPE. Match the phlebotomy materials found in Column A to the Collection Method found in Column B. Column A: Materials 1. Cotton 2. Plain glass tube3. Red top glass tube4. Gauge 23 syringe5. Two-way needle6. Adapter7. Lancet8. Lavender top plastic tube9. Tourniquet10. Micropore Column B: Collection MethodA. Syringe MethodB. Evacuated SystemC. Winged CollectionD. Capillary CollectionE. All of the aboveF. Syringe and Evacuated onlyG. Evacuated and Winged

Case Study: Lab Errors

plz answer all the questions plz and also state the aim for this lab .. thank u

Activity 6. Case Digest Read and analyze the situation. Answer the following questions briefly as possible. Five customers got ill after eating cooked mussel from a 3-Michelin star restaurant in Manila. Nausea, vomiting, and bloody diarrhea were observed among the patients. The patients were then diagnosed to have gastroenteritis. Several factors were associated to the occurrence of the situation. First, food handlers had poor personal hygiene. Second, the food source was not verified. Third, the mussel was not cooked immediately or stored in proper storage after it was delivered in the restaurant and lastly, food handlers were using the same utensils with other variety of food. Онestions: 1. The presence of foodborne illness can result to greatest danger to individuals and establishment. Specify five possible dangers it may brought to: a. Individuals b. Establishments 2. Identify the hazard that causes the illness. How should this hazard be prevented?

EXPERIMENT 2 Title: Aseptic technique  Objectives: To apply the aseptic technique To observe the growth found on the petri dish. Material/apparatus: Gloves, incubator, soap for handwashing, tissues, nutrient agar, 70% alcohol Procedure: Wipe off the bench with 70% alcohol. Draw a line down the middle of the petri dish to divide the plate in half. Label each halves with A and B. Press your unwashed thumb onto the agar at column A. Apply proper handwashing technique. Put the same thumb after washing onto agar at the column B. Incubate the petri dish at 37°C for 24 hours or overnight. Observe and note down the colonies. Results:

Separation of Amino Acids by Thin Layer Chromatography Lab Questions 4. Why is it necessary to run TLC in a closed container and have the chamber saturated with solventvapor? 5. Why must the spot be applied to the TLC plate above the level of development solvent? 6. What will be the result of adding too much sample to the TLC plate?

Please answer the questions in the grey colored box provided in the image.

I need help with microbiology/2010 1. Describe where the following items should be discarded: a) gloves b) petri dishes   c) test tubes   d) microscope slides   2. Describe the safety procedures for the following hypothetical situations: a. You spilled a full test tube of bacterial culture on the bench top.   b. You notice flames coming from the tubing of your Bunsen burner

Topic: Reducing and Non Reducing Carbohydrates Among the Benedict's, Reagent, and Iodine tests, which is most highly applicable in biomedical field? Explain

Test I. Identification Instruction: Refer to the micrographs below in answering the questions. EST B 1 3.

True or False 1. Two challenges regarding IFU are adhering with the IFU's and interpreting complex IFU. 2.  Mechanical disinfection is typically more effective than manual techniques. 3. Adding a UV disinfector to a pass-through window is NOT offer a higher level a safety for sterile processing personnel.

Please explain and cite references if possible. Thank you! 1. Explain why stool specimen must not be frozen nor placed in incubators.

TOPIC: KATO-THICK & KATO-KATZ TECHNIQUES What are the differences between Kato-Thick smear and Kato-Katz technique? Briefly explain each.

Topic:  Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition.  After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.

covert the procedure to passive voice and past tense

Complete both pages correctly

question below is the reference you can also add the procedure but i need the answer most importantly and use the reference if you need it , draw it please for me to understand better

labeling excercise in phlebotomy

Summary the Procesure below.

bacteriophage lab report:

INSTRUCTIONS: Please do not copy here in Bartleby or in Google. QUESTIONS : 1. What is the importance of the aseptic technique? Explain.

Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9

BIOL2201, S24 Dissection 3- Sheep Heart Good source: https://www.youtube.com/watch?v=-ZbXiOrlFJI External Anatomy 1. Set up your dissection in a way you feel comfortable. Use the included video for a suggested method. 2. Make sure to have a camera ready to take pictures of your brain to show you completed the dissection. You will need at least 3 pictures of the eye, more are fine. You will need to identify all of the bolded structures below. 3. Identify the right and left sides of the heart. Look closely and on one side you will see a diagonal line of blood vessels that divide the heart; this line is called the interventricular sulcus. The half that includes all of the apex (pointed end) of the heart is the left side. 4. Locate the coronary arteries and veins that are on the surface of the heart. 5. Find the flaps of dark tissue on the top of the heart. These ear-like flaps are called auricles. 6. The front-most vessel is the pulmonary trunk. Place a probe or pin in the vessel to mark…