Lab4 Lab Report

Instructions: Read 13-2 Manipulating DNA pages 322-323. As you read each section, examine the figures and captions (explanations). Identify any questions you may have. 1) Develop an analogy for the processes researchers use to make changes to DNA. In yo analogy, explain how it is similar to the techniques used in genetic engineering. You can draw a graphic organizer, make a table, or write a few sentences describing your analogy. 2) Devise flowchart that shows the steps to prepare DNA for gel electrophoresis, as well the protocol for setting up and running a gel. You can add diagrams to the flowchart an add detailed notes if you like. English (inited Sate) O Focs ere to search 4 CO RU G\ L B. 2N A\ Alt Ciri

question:  What is the purpose of adding table salt into the solution? What reagent is used in research labs/ Is it also table salt (NaCl)?

Question:- Is DNA sequencing a in vivo, in vitro and/or in silico?   What product(s) is/are formed in DNA sequencing and how and where would the reaction begin? Also, what raw materials are needed?

Gel Electrophoresis Background and Protocol: Gel electrophoresis is a laboratory technique that separates molecules by size using an electric current.  The test has a positive and negative side.  Do you believe DNA should be loaded on the positive (red) side or the negative (black) side?  Please explain why using scientific reasoning. Will larger DNA bands be closer or farther away from the well where you administered samples?  Why?

Biol370 - Assignment #2 – Spring 2020: Replication - Transcription - Translation Due 3/10/2020 (Before the beginning of the class) Name ID Instruction: 1. Print this document (US-letter only) Fill the table below with the appropriate color (see coloring rule) 3. Complete and annotate the drawing in attachment (see page #2). Make sure all the listed terms are present in your final drawing! (graded using 5 randomly selected terms 2. This type of drawing makes only sense for Eukaryotes/Prokaryotes (circle one) because: or The annotation must follow the coloring rule : DNA in BLACK. RNA in RED. Proteins and AAs in GREEN. Complex structures with some DNA and/or RNA and/or Proteins in BLUE. Color Color Color DNA DNA pol III Stop codon RNA RNA polymerase 5'-UTR Peptide Helicase 3'-UTR Leading strand Topoisomerase CDS Lagging strand Ligase RBS Coding strand Primer Ribosome SSU Template strand -10/-35 Box Ribosome LSU 5'/3' ends (all) Transcription start AA-TRNA N-/C- terminal ends…

erm test Fall 2020 (page 1 of x A elearn.squ.edu.om/mod/quiz/attempt.php?attempt31335328&cmid%3D697149 -aming System (Academic) Fon 3 "DNA is passed from one generation to another during cells division after DNA replication". et red which of the following statements regarding DNA replication is NOT correct: d out of Select one: O a. Each DNA strand of the double helix will be copied g question O b. The newly synthesized strand will have the complementary sequence of the parental template strand O c. The daughter cell will have doub the amount of DNA that the parental cell had n 4 Which of the following ketoses contain the same number of carbons as glucose? ed Select one: out of O a. Sucrose O b. Fructose question O c. Ribose O d. Galactose O e. Aldose 5) helps maintaining the skin's cells and prevents the growth of some

Procedure: 1. Using the DNA provided transcribe DNA into mRNA. 2. Use the mRNA strand you created and break it up into codons. 3. Plug the codons into the amino acid chart to determine the correct amino acid needed to build that protein. 4. Identify the protein you made by comparing the sequence to the pictures 5. Answer the questions for each protein molecule you build before moving on to the next. Protein 1: DNA A AGACCGTATAC mRNA Amino Acid Sequence 1. Which kind of protein molecule did this gene make? 2 How does this protein help the body maintain homeostasis2

DNA EXTRACTION Why do scientists isolate DNA?                                                                                                                    From what part of the subject’s body were cells collected? Give the specific purpose of each experimental step:           Lysis solution & heat:                                                                                             Salt & centrifugation:                                                                                  After centrifuging, where in the tube is the DNA? What is the purpose of adding alcohol?

Question:- compare these two techniques. Compare a nucleosome protection assay and a northern blotting is a text format and also in drawinv format. for drawing use same sample for both techniques

Post lab questions 1) The approximate length of a DNA double helix is around 0.34nm per base pair. If the extracted DNA was in the form of a single double helix, calculate how long it would be in meters. (1nm = = 10-⁹m) Clear All Formatting 2) How long would it be in miles? (1mile = 1609.34m) 3) Is your double helix longer than the diameter of the Earth? 4) Is your double helix longer than the circumference of the Earth?

Solve 5.

Legrning Task 2: Make a.model of a DNA template to deternmine the seguence of bases in th new DNA strahds Then, answer the gulde questions that follow. Materials: crayons. Scissors, paste/tape, used folder or illustration board Procedure: Use the pattern of the DNA templater (attached to this LP). Color code, phosphate = blue, deoxyribose sugar = green, nitrogenous base as follows: adenine= yellow, thymine = pink, guanine = violet, cytosine = red. And cut the shapes of each nucleotides. Buld a model of a strand of a DNA molecule. The strand should contain 6 base" rungs" following the given order of the nucleotides: Guanine, Adenine, Cytosine, Thymine, Cytosine, Guanine. Tape the cutout pattern to form the nucieotides. This will represent the left half of DNA. Make a complementary strand that you made in step 3. Tape the cut -out pattern again forming the nucieotides for the second strand of the DNA molecules. Match the bases of the first strand and the second strand. Do not tape across…

Asap please explain

DNA Extraction by Alkaline Lysis Procedure: 1. Spin 1.5 ml of cells in a microcentrifuge at maximum speed (12,000 rpm) for 20s to pellet. Remove the supernatant completely with a Pasteur pipet or a plastic pipettor tip. The spins can be performed at 4C or at room temperature. Longer spins make it difficult to resuspend cells. 2. Resuspend pellet in 100µl GTE solution and let sit 5 min at room temperature. Be sure cells are completely resuspended. 3. Add 200µl NaOH/SDS solution, mix by tapping tube with finger, and place on ice for 5 min. 4. Add 150µl potassium acetate solution and vortex at maximum speed for 2s to mix. Place on ice for 5-15 min. Be sure mixing is complete. 5. Spin 3 min at 12,000 rpm to pellet cell debris and chromosomal DNA. 6. Transfer 0.4 ml supernatant to a fresh tube, mix it with 0.8 ml of 95% ethanol or 0.4 ml isopropanol, and let sit 2 min at room temperature precipitate nucleic acids. 7. Spin at 12,000rpm for 3 min at room temperature to pellet plasmid DNA and…

Give detailed Solution with explanation (no need Handwritten answer

Question:- Your friend works in a lab that studies origin licensing. He is particularly interested in the pre-replicative complex (pre-RC) and has isolated a temperature-sensitive yeast mutant that does not seem to assemble the pre-RC at the origins of replication. However, he has gotten into an argument with a new student in the lab. The student thinks that this yeast mutant will arrest in late mitosis or early G1, because that is when the pre-RC is normally assembled. Your friend disagrees. Who is right, and why?

This is homework for a practice assessment. I need some assistance with these questions, so I can use them for studying material. Thank you so much! I highly appreciate it!

DNA Extraction by Alkaline Lysis Procedure: 1. Spin 1.5 ml of cells in a microcentrifuge at maximum speed (12,000 rpm) for 20s to pellet. Remove the supernatant completely with a Pasteur pipet or a plastic pipettor tip. The spins can be performed at FC or at room temperature. Longer spins make it difficult to resuspend cells. 2 Resuspend pellet in 100pul GTE solution and let sit 5 min at room temperature. Be sure cells are completely resuspended. 3. Add 200ul NaOH/SDS solution, mix by tapping tube with finger, and place on ice for 5 min. 4. Add 150ul potassium acetate solution and vortex at maximum speed for 2s to mix. Place on ice for 5-15 min. Be sure mixing is complete. 5. Spin 3 min at 12,000 rpm to pellet cell debris and chromosomal DNA 6. Transfer 0.4 ml supernatant to a fresh tube, mix it with 0.8 ml of 95% ethanol or 0.4 ml isopropanol, and let sit 2 min at room temperature precipitate nucleic acids. 7. Spin at 12,000rpm for 3 min at room temperature to pellet plasmid DNA and…

Question:- When large genomes are sequenced, which of the following is true? Group of answer choices   The genomes are converted from RNA to cDNA using reverse transcriptase, and then the cDNA is sequenced.   The genomes are fragmented, the DNA fragments are sequenced by any number of methods, and the sequences assembled to provide the original complete genome.   Sanger dideoxy sequencing is never used – instead, only nanopore sequencing is used.   The assembled sequences must have all gaps closed if the genome is to be useful for the research community.

Remodeling complexes use the energy from ATPhydrolysis to change the position of nucleosomes,exposing promoters and allowing gene ____________.

Instruction - Please answer them correctly - Please answer all of them, they are connected. MUTATION Fill in the correct nucleotide base pairing and amino acid sequence of the mutated DNA a. What is the 3’-5’ DNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) b. What is the mRNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) c. What is the tRNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) d. What is the amino acid sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) e. What is the most convincing type of mutation had occurred? (Frameshift resulting Missense; Frameshift resulting Nonsense; Substitution – Silent; Substitution – Missense; Substitution – Nonsense)

Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes.  Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…

5) Answers to the following questions. A. You are given a tube containing 275 ng of purified PCR product (DNA) that is 1262 bp long. How many picomoles of PCR product are in the tube? The average weight of a deoxynucleotide monophosphate is 328 g/mol. B. You digest a 5 kb plasmid with two enzymes that yield fragments of 4 kb and 1 kb, and resolve the digest on the gel. What would you predict to see in terms of the intensities of the bands?

Questions are talking about amplification of DNA When we run the gel, we also run a ladder why? what information does that give us. why does it form a ladder shape on the gel (what is happening to cause things to separate out in many bands)?

QUESTION:- Whole genome sequencing provides the most comprehensive genomic information. Nevertheless, there are many instances when microarrays or limited sequencing of a selected panel of genes are the approaches chosen for clinical use , Why?

Question:- Many cancer treatment drugs specifically target DNA synthesis/replication. Find a drug that is currently used in cancer treatment Tell me what it is used for (e.g. any specific types of cancers?) What specific DNA process does it target? From a molecular biology perspective (what you have learned so far), what are the negative outcomes of targeting this process?.

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