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Biology
Date
Jan 9, 2024
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N
AME
:
C
HEEK
C
ELL
DNA-L
AB
R
EPORT
S
HEET
Exercise 1 – Isolating Cheek Cell DNA
Analysis of Results
Appearance of Solution
What is happening to the cells/DNA?
After lysis buffer
Cloudy light blue solution
After adding the lysis buffer there was a separation between the buffer and my DNA at 3mL. After adding the Protease solution and inverting it 5x’s, mixture was a pale cloudy blue throughout.
After alcohol added
Solution turned cloudy
After adding alcohol there was a noticeable
separation of the DNA from the alcohol at 6mL. While observing the solution a very thin layer of white formed at the bottom of the test tube.
After alcohol mixed
Solution turned to a light cloudy color at the bottom, clear solution at the top
After mixing observed separation between 5.5 and 6mL and another slight separation of the DNA at 2mL. From 5.5 to 10mL solution was almost clear and from 2mL to the bottom of the test tube solution was a light cloudy color. I observed at
the bottom of the test tube there was a white stringy substance with small white particles floating in the solution.
Questions:
1. Why is cell lysis the first step in isolating DNA from your cells?
The lysis buffer aids in the chemical breakdown of the cell membranes. 2. Lysis buffer typically contains detergent. Why would this help to lyse the cells?
The dish soap in the lysis buffer is responsible for dissolving the hydrophobic (oil-based) molecules.
3. Decribe the appearance of your DNA. Is its appearance consistent with what you know about the structure of DNA? Why or why not?
When observing the appearance of my DNA it looked like a stringy white small blob. My DNA does not look like a double helix, because the double helix is microscopic. It looked like a stingy clump of white mucus
4. If you wanted to isolate a copy of the gene that codes for a protein found in the brain, could that gene be located in cheek cells? Explain your reasoning.
I would be able to isolate a copy of a gene that is found in the brain by the cheek cells, because all cells in our body have the same genetic composition.
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Related Questions
to 4 minutes)
The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated
31
parental duplex
5'
3'
fork progression
v A
1Lagging strand
2. An Okazaki tragment
3.Site of action of DNA topoisomerase
4 Leading strand
5. Site of action of DNA helicCase
6.Site of action of DNA ligase
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1e please
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Please help me? immediately
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erm test Fall 2020 (page 1 of x
A elearn.squ.edu.om/mod/quiz/attempt.php?attempt31335328&cmid%3D697149
-aming System (Academic)
Fon 3
"DNA is passed from one generation to another during cells division after DNA replication".
et
red
which of the following statements regarding DNA replication is NOT correct:
d out of
Select one:
O a. Each DNA strand of the double helix will be copied
g question
O b. The newly synthesized strand will have the complementary sequence of the parental template strand
O c. The daughter cell will have doub the amount of DNA that the parental cell had
n 4
Which of the following ketoses contain the same number of carbons as glucose?
ed
Select one:
out of
O a. Sucrose
O b. Fructose
question
O c. Ribose
O d. Galactose
O e. Aldose
5) helps maintaining the skin's cells and prevents the growth of some
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Could you please assist?
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please focus on this question 3 times i submitted it and three different answers, please make sure of ur answer
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Give detailed Solution with explanation (no need Handwritten answer
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PLEASE I ASKED 3 TIMES ON BARTELBY AND LITERALLY EVERONE GAVE A DIFFERNT ANSWER, I PROVIDED PROOF THAT THIS HAPPENED SO YOU DONT THINK IM LYING SO PLEASE READ THE QUESTION AND ANSWER CAREFULLY
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nd 2 minutes):
Any RNA polymerase in any organism:
O A Synthesizes RNA chains in the 3 to-5" direction
O B. Binds tightly to a reqion of DNA located thousands of base pairs away from the transcobed rogion of the DNA
OC Has proofreading activity
O D. Separates DNA strands throughout a long region of DNA (up to tinousands of base pairs) and then copies one of them.
OE Has a subunit called A (lambda), which acts as a proofreading ribonuclease
OF. Can initiate synthesis of a new RNA chain without a primer
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nand portable.
6.5 mm micro-edge
16405U processon
USE YOUR SMARTPHONE
w micro-edge display
design
See dnclamers on product bo
Revi
Vide
Fea
Sameness and Variety (Mitosis and Meiosis)
161
Spe
Example
Sup
SCAN
Expressed using apples, DNA replication looks like this.
Go look at the model of DNA on the demonstration table.
We have been presenting DNA as a straight ladder, but actually it is
twisted on itself like a spiral staircase. This shape is called a heliz.
Observe
The Chromosome
Normally DNA exists as loose strands (chromatin) in the nucleus of
a cell. This nuclear DNA sends a message (RNA) to the ribosomes where
protein and enzymes are synthesized.
When stretched out, the length of one DNA molecule in a human cell
is almost 4 cm. However, the cell itself is but a tiny fraction of that size.
During cell reproduction the DNA must be able to move around. So it
shortens its length by tightly coiling up. In doing so, the DNA strands
become wider and are visible under a microscope.…
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practice test i don't know what this means
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The correct option is D.
But I want you to explain why the other options are incorrect. Thanks
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common-assessment-delivery/start/4797160920?action=onresume&submissionld%-468979669
English 9 Student D.
S ddddddddddddddd...
Type of Genetics Cr.
S TEWWG Chapter
DNA Protein Synthesis Test
Chromosome
Free Nucleotides
DNA Polymerase
Leading Strand
Original-
(Template
DNA)
Helicase
Lagging
Strand
Replication
Fork
Adenine
Thymine
Guanine
Cytosine
DNA Polymerase
Original (Template) DNA Strand
1. This diagram is showing the process of
2. 1f the original template strand reads: TTACGCAGT, DNA polymerase would add nucleotides in the order:
4 5 6
7
8.
9 10
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Answer they last two questions
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please help with practice problems
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Answer:
giycogen
Time left 0:4T:-
If you have got the following DNA template molecules, which one of them will require more energy to break down the hydrogen bonds between
the antiparallel strands?
O a.
GCGCGCGCGCGCGCGCGCGCG
O b. GGGGGCCCCCAATTCCCCCCC
O c. AAAAAATTTTTCCCCCGGGGG
O d. ТAСТАСАСTGTGGTTAАТТААА
Oe.
АТАТАТАТСGСGTTAAATTCTА
CLEAR MY CHOICE
Match the given words with the most suitable words from the given list.
Unicellular fungi
Hyphae
Choose...
Охуgen
Atom
ENG
سطح المكتب
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Please asap
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Is this correct if not please tell me what are the correct answers.
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i need to do it one
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Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical?
Summary of Qiagen DNA extraction steps
Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
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can someone help me with number 5?
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ans fast I will Upvote
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Formation ofa recombinant DNA molecule.
GAATTC
GAATTC
CITAAG
CITAAG
double-stranded
DNA
DAATTO
DAATTO
CTTAA
OG
CTAA
AATT
GI AATT
CTTAA
GAARIC
CTTAAG
The enzyme that cataly zes the joining of fragments to form recombin ant DNA is:
DNA polymerase
DNA ligase
helicase
re striction endonuclease
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answere is wrong i need help identifying the right otder in where they go
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First part is right need help with the second part please
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Third letter
UCAG
UCAGH CAC
First letter
Part 5: Coding Practice
1. Use this sequence of DNA to answer the following former test questions:
5'-- TTAATGGGACAGCTTGTGTAGAGG --3'
a. What is the complementary strand of DNA?
b. Using the complementary strand of DNA (your answer from part a) as the template
strand, what is the transcribed mRNA sequence?
C. What is the amino acid sequence translated from the strand of mRNA synthesized in
part b (use the genetic code below)?
Remember:
i. Start codon!
ii. Stop codon!
bac
ocent
Seond letter
UUU Phe
UAU Tyr
UGU
UGC
Cys
UUC
UCC
UAC
Ser
UAA Stop UGA Stop A
UAG Stop UGG Trp G
C.
UUA
UCA
UUG
Le
UCG
[
CAU ]
CAC
CUU
CGU
His
CUC
C
CGC
ne.
CCA
Arg
Pro
CUA
CAA
CGA
CCG
CAG Gin
CGG
CUG
AAU 1
AAC Asn
ACU
AGU
[
[
AUU
AUC Ile
AGC Ser
Thr
A
AUA
AGA
AGG
ACA
AAA
Arg
AUG Met ACG
AAG
GUU
GCU
GAU
GGU
Asp
GAC
GCC
GGC
GUC
Val
G GUA
GCA
Ala
GAA
GGA
Gly
GAG Glu
GGG
GUG
GCG
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I need help answwering the queshtions
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sampies
B.
In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of DNA?
O A. A
O B. B
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15
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d. Biuret test
O e. NaOH
Clear my choice
In the method to isolate the DNA from peas, you used the centrifuge right after the addition of
salts and alcohol; in which part of the tube will you find the stringy white DNA?
of
Select one:
stion
a. Suspended in the solution
O b. At this step, no DNA will be visible
O c. Top of the tube precipitate
O d. Bottom of the tube precipitate
Clear my
choice
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I need. To understand 22 A and 22 b please
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AKS 5c: Which student correctly explained what is occurring in the images?
AEGCEE ECCUAE
FACGAAAGCAEA
3'
Met Leu Ser Tyr
Tyr Gle Ser le
Met Leu Ser Tyr
Tyr Glu Ser le
The 4th student explains the first arrow must demonstrate how the base pairing rule
is being used to replicate a strand of DNA since the complementary pairs are being
O lined up for semi conservative replication. The second arrow must demonstrate the
process of transcription since the DNA is being read at the ribosomes and the
monomers of protein are being connected.
The 1st student explains the first arrow must demonstrate how the base pairing rule
is being used to replicate a strand of DNA since the complementary pairs are being
lined up for semi conservative replication. The second arrow must demonstrate the
process of translation since the RNA is being read at the ribosomes and the
monomers of protein are being connected.
The 2nd student explains the first arrow must demonstrate how the base pairing rule
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MUTATION:
Fill in the correct nucleotide base pairing and amino acid sequence of the mutated DNA
-Answer/fill the blank circles provided in the image.
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3 of 11
Following agarose gel electrophoresis, where on the gel will the largest DNA molecules be, and why?
O Near the bottom because they carry more negative charge
O Near the bottom because the migrate through the matrix of the gel faster
Near the top because they can not migrate through the matrix of the gel as fast
O Near the top because they are less negatively charged
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Related Questions
- to 4 minutes) The schematic diagram below shows organization of the DNA replication fork. Match parts of the diagram (labeled A-F) with the corresponding term from the answer list (designated 31 parental duplex 5' 3' fork progression v A 1Lagging strand 2. An Okazaki tragment 3.Site of action of DNA topoisomerase 4 Leading strand 5. Site of action of DNA helicCase 6.Site of action of DNA ligasearrow_forward1e pleasearrow_forwardPlease help me? immediatelyarrow_forward
- erm test Fall 2020 (page 1 of x A elearn.squ.edu.om/mod/quiz/attempt.php?attempt31335328&cmid%3D697149 -aming System (Academic) Fon 3 "DNA is passed from one generation to another during cells division after DNA replication". et red which of the following statements regarding DNA replication is NOT correct: d out of Select one: O a. Each DNA strand of the double helix will be copied g question O b. The newly synthesized strand will have the complementary sequence of the parental template strand O c. The daughter cell will have doub the amount of DNA that the parental cell had n 4 Which of the following ketoses contain the same number of carbons as glucose? ed Select one: out of O a. Sucrose O b. Fructose question O c. Ribose O d. Galactose O e. Aldose 5) helps maintaining the skin's cells and prevents the growth of somearrow_forwardCould you please assist?arrow_forwardplease focus on this question 3 times i submitted it and three different answers, please make sure of ur answerarrow_forward
- Give detailed Solution with explanation (no need Handwritten answerarrow_forwardPLEASE I ASKED 3 TIMES ON BARTELBY AND LITERALLY EVERONE GAVE A DIFFERNT ANSWER, I PROVIDED PROOF THAT THIS HAPPENED SO YOU DONT THINK IM LYING SO PLEASE READ THE QUESTION AND ANSWER CAREFULLYarrow_forwardnd 2 minutes): Any RNA polymerase in any organism: O A Synthesizes RNA chains in the 3 to-5" direction O B. Binds tightly to a reqion of DNA located thousands of base pairs away from the transcobed rogion of the DNA OC Has proofreading activity O D. Separates DNA strands throughout a long region of DNA (up to tinousands of base pairs) and then copies one of them. OE Has a subunit called A (lambda), which acts as a proofreading ribonuclease OF. Can initiate synthesis of a new RNA chain without a primerarrow_forward
- nand portable. 6.5 mm micro-edge 16405U processon USE YOUR SMARTPHONE w micro-edge display design See dnclamers on product bo Revi Vide Fea Sameness and Variety (Mitosis and Meiosis) 161 Spe Example Sup SCAN Expressed using apples, DNA replication looks like this. Go look at the model of DNA on the demonstration table. We have been presenting DNA as a straight ladder, but actually it is twisted on itself like a spiral staircase. This shape is called a heliz. Observe The Chromosome Normally DNA exists as loose strands (chromatin) in the nucleus of a cell. This nuclear DNA sends a message (RNA) to the ribosomes where protein and enzymes are synthesized. When stretched out, the length of one DNA molecule in a human cell is almost 4 cm. However, the cell itself is but a tiny fraction of that size. During cell reproduction the DNA must be able to move around. So it shortens its length by tightly coiling up. In doing so, the DNA strands become wider and are visible under a microscope.…arrow_forwardpractice test i don't know what this meansarrow_forwardThe correct option is D. But I want you to explain why the other options are incorrect. Thanksarrow_forward
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SEE MORE QUESTIONS
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Recommended textbooks for you
- Biology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStax
Biology 2e
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ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
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