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Biomedical Engincering Dept. Biocompatibility Hello sir, I know it takes precious Lab. Sheet-Level time. Please, I have a report on this Semester 1 subject. Only 5 papers within a Experiment Title: Cytotoxicity Test discussion solution. I want to solve the text of the keyboard. Thank you, Experiment no. 2 sir 1 12:34 PMA Objectives: Study the eytotoxicity and how it is measured. 1- Background Cytotoxicity can lead healthy living cells to three potential cellular fates. 1. Necrosis (accidental cell death): Rapid loss of membrane integrity and cell lysis 2. Apoptosis (programmed cell death): slower, more orderly, and genetically controlled 3. Cytostasis (a decrease in cell viability): cells remain alive but fail to actively grow and divide Importance of measuring cytotoxicity two major reasons 1. Either you want specific cells to die and look for an adequate compound/condition (cancer and immunotherapy) 2. Or you want to exclude cytoloxicity in specific cells (chemicals and drugs)…

ull MTN-Stay Safe LTE 10:13 AM @ 34% #3 Mol Bio Gene o... Complete the following tasks. You discovered that a species of bacteria can break down Styrofoam™ (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR compon briefly but completely. Answer DNA polymerase isolated from Thermus aquaticus а. Answer: b. Deoxynucleotide triphosphates (DNTPS)…

BONUS (15 points) The fallowing series of dilution was prepared from a specimen to determine the number of bacteria. There were 62 colonies on the agar plate prepared by transferring 0.3 ml from the tube number 4. Calculate the dilution factors for each tube. What is the cell concentration in the original specimen? Calculate the total number of cells in tube number 2. étv A) F12 F9 F10 F7 % & %3D delete 5 { T Y J K

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TOPIC: E. coli T4 (T4 Bacteriophage) Include a maximum of 3 sentences, a brief description of their growth. What are the structures responsible for growth and reproduction? What are their physical and nutritional (or chemical) requirements?

Helping tags: Biology, bacteria, lag phase WILL UPVOTE, just pls help me answer the following questions and explain them clearly. Thank you. TOPIC: Bacterial Growth Curve 1. How may the following growth and culture conditions affect the length of the lag phase? EXPLAIN YOUR ANSWERS. a) inoculum is from an old culture b) shifting cells from rich culture medium to a poorer one c) exponentially growing culture is transferred into the same medium under the same growth conditions

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Average plaques for bacteriophage A，B，C are 137，36，25. PFU/ml is average plaques multiply by the volume dilution multiply by the dilution factor. Show your working for each bacteriophage.

question: Can you summarize and explain for me what you want to tell in the article below? When I read it myself, I do not understand exactly what is meant by the article. It would be nice if you could highlight the important points. You can use them in a figure or diagram to explain. thank you and hava a nice day :) Article: Nanomaterial-Based Vaccine Development and Immunomodulation Following the publication of the genetic sequence of SARS-CoV-2 on January 11, 2020, intense research efforts have been devoted to developing a vaccine against COVID-19. With unprecedented speed, this extraordinary scientific mobilization led the first vaccine candidate to enter the Phase I human clinical trial on March 16, 2020, and other novel candidates are rapidly following. Up to May 22, 2020, there are 10 COVID-19 candidate vaccines in clinical evaluations and 114 in preclinical development.  Concerning vaccine and immunization research, nanomaterials can assist in multiple ways to boost the…

Please answer fast One hundred and forty seven plaques were counted from a 10-7dilution plate when 0.1 ml of bacteriophage-infected bacteria was plated. What was the original bacteriophage density?

Is Table Window Help 1 Exam_2_problem_set_new- Saved to my Mac Design Layout References Mailings Review View AaBbCcDdF AaBbCcDdF AaBbCcDd AaB Normal No Spacing Heading 1 Не s, fixes, and improvements, choose Check for Updates. 2. Calculate the effective dilution. You were given a sample which contained 5.5mL of bacterial culture. You added 10uL of phage solution to the culture. What was the effective dilution produced by the addition of the phage solution?

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shift T ctrl caps lock tab VILION fn Quiz Submissions - Take: Unit D Practice Quiz JUDITH BIRECH (username: nj2191ne) Attempt 1 Written: Feb 9, 2023 2:20 PM - Feb 9, 2023 3:06 PM Submission View Released: Feb 13, 2023 12:01 AM Question 2 A patient had decreased mast cells. Which do you predict? K a) decreased histamine & increased inflammation Ob) increased histamine & increased inflammation 34°F Sunny /" @ Q ion x360 Laptop ment anywhere A Z W ■ 3 S E 4 alt % الالالال التاكاك R O Search 5 F T 6 8 9 ||G|H|J|K M

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Pipetting 1. Explain why a micropipettor is an important instrument in biochemistry labs. 2. Describe the basic components (and function) of a micropipettor. Bacterial Techniques 1. Define the following. (a) Serial Dilution (b) Streak Plating (c) Spread Plating Transformation 1. Define bacterial transformation and explain why it is an important method in biochemistry labs. 2. Describe (figure, narrative) a plasmid and describe the basic components of a plasmid. 3. What role does CaCl2 play in bacterial transformation? 4. What role does heat shock play in bacterial transformation? Plasmid Isolation 1. Describe the roles the following play in plasmid purification. (a) Lysis Buffer (b) Neutralization Buffer (c) Elution Buffer

Activity 2. You COMPLETE Me! Objective: Identify the methods used for inserting plasmids into the host cells. What you need: pen and paper What to do: On a separate sheet of paper, copy and complete the key points of the methods used to introduce plasmids into the host cells. Choose your answers from the box gene gun biolistics electric shock mammalian cells plasmid insertion by Heat Shock Treatment increase the pore sizes of their plasma membranes (2) competent plasmid DNA Heat Shock electroporation There are three methods on introducing plasmids into the host cells namely: (1)_ (3) Biolistics uses a (4)_ to fire DNA-coated pellets on plant tissues. Plasmid insertion by Heat Shock Treatment is a process wherein target cells undergo a The pretreatment is said to make the cells (6). pretreatment procedure to (5) the introduction of the (7)_ plasmid at about 4°C for about 30 minutes and the plasmids concentrate near the cells during After the pretreatment, the cells are incubated with…

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Discusses the methods to -1 optimise fluorescent cellular (staining (priority first List the freezing -2 parameters of the slow cooling technique Parameter of verification -3 precedure of freezing 9

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16. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X

Required information Skip to question The Gram Stain This animation learning module will review the Gram stain technique, a differential staining process that distinguishes between bacteria based on their cell wall composition. Complete the following activities to demonstrate your understanding of this important technique in microbiology. 1. The differential stage of the Gram stain is the application of . multiple choice 1 ethanol crystal violet iodine safranin 2. If the Gram's iodine step was skipped in Gram stain procedure, what color would the cells likely be when viewed under the microscope? multiple choice 2 Most cells would appear red/pink All cells appear colorless Most cells appear purple Most cells would appear blue 3. True or False: All bacteria can be classified as either gram-positive or gram-negative (Click to select) 4. True or False: The presence of flagella can be determined by a Gram stain. (Click to select) 5. Rod - shaped bacteria multiple choice 3 are all gram -…

Graph SEQ Graph ARABIC I p24 po/ml 10 7. 3) Quantitative skills: Using the graph below, determine the approximate concentration of p24 capsid protein (in kg/L) after 6 days of transfection with the 168.1/R molecular clone. 188.10 10 188.VR Include your calculations within the following space: 168.1 108.VT 10 10 10 10 10 12 14 days after transfection

uestion Completion Status: - A Moving to the next question prevents changes to this answer. destion 3 In the case of Escherichia coli, commonly abbreviated E. coli, the name coli is known as the name of the bacteria. genus O formal O last O first species A Moving to the next question prevents changes to this answer.