Lab 6 - Cracking the Genetic Code - Fall 2023

Biol370 - Assignment #2 – Spring 2020: Replication - Transcription - Translation Due 3/10/2020 (Before the beginning of the class) Name ID Instruction: 1. Print this document (US-letter only) Fill the table below with the appropriate color (see coloring rule) 3. Complete and annotate the drawing in attachment (see page #2). Make sure all the listed terms are present in your final drawing! (graded using 5 randomly selected terms 2. This type of drawing makes only sense for Eukaryotes/Prokaryotes (circle one) because: or The annotation must follow the coloring rule : DNA in BLACK. RNA in RED. Proteins and AAs in GREEN. Complex structures with some DNA and/or RNA and/or Proteins in BLUE. Color Color Color DNA DNA pol III Stop codon RNA RNA polymerase 5'-UTR Peptide Helicase 3'-UTR Leading strand Topoisomerase CDS Lagging strand Ligase RBS Coding strand Primer Ribosome SSU Template strand -10/-35 Box Ribosome LSU 5'/3' ends (all) Transcription start AA-TRNA N-/C- terminal ends…

Instruction - Please answer them correctly - Please answer all of them, they are connected. MUTATION Fill in the correct nucleotide base pairing and amino acid sequence of the mutated DNA a. What is the 3’-5’ DNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) b. What is the mRNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) c. What is the tRNA sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) d. What is the amino acid sequence? (FORMAT: XXX-XXX-XXX-XXX-XXX) e. What is the most convincing type of mutation had occurred? (Frameshift resulting Missense; Frameshift resulting Nonsense; Substitution – Silent; Substitution – Missense; Substitution – Nonsense)

Instructions: Read 13-2 Manipulating DNA pages 322-323. As you read each section, examine the figures and captions (explanations). Identify any questions you may have. 1) Develop an analogy for the processes researchers use to make changes to DNA. In yo analogy, explain how it is similar to the techniques used in genetic engineering. You can draw a graphic organizer, make a table, or write a few sentences describing your analogy. 2) Devise flowchart that shows the steps to prepare DNA for gel electrophoresis, as well the protocol for setting up and running a gel. You can add diagrams to the flowchart an add detailed notes if you like. English (inited Sate) O Focs ere to search 4 CO RU G\ L B. 2N A\ Alt Ciri

Questions are talking about amplification of DNA When we run the gel, we also run a ladder why? what information does that give us. why does it form a ladder shape on the gel (what is happening to cause things to separate out in many bands)?

QUESTION:- Whole genome sequencing provides the most comprehensive genomic information. Nevertheless, there are many instances when microarrays or limited sequencing of a selected panel of genes are the approaches chosen for clinical use , Why?

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Primer Designing:

Question:- Your friend works in a lab that studies origin licensing. He is particularly interested in the pre-replicative complex (pre-RC) and has isolated a temperature-sensitive yeast mutant that does not seem to assemble the pre-RC at the origins of replication. However, he has gotten into an argument with a new student in the lab. The student thinks that this yeast mutant will arrest in late mitosis or early G1, because that is when the pre-RC is normally assembled. Your friend disagrees. Who is right, and why?

Please check the right answers. I’m not sure

The team would now like to establish the smallest possible deletion that would inactivate the function of the protein. Describe a strategy to carry out this experiment To check their sequences, the team choses to carry out DNA sequencing themselves, using the Sanger method. Describe the biochemical processes involved in this technique in detail.

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This is homework for a practice assessment. I need some assistance with these questions, so I can use them for studying material. Thank you so much! I highly appreciate it!

In relation to DNA Isolation experiment 1. Once the tissue has been ground and heated to 60°C, the DNA is released into the solution, but so are many other types of cellular molecules. List some types of molecules besides DNA that you would expect to find in a cell. 2. What is the role of the detergent in this protocol? How does it perform this function? 3. Name two important functions of the proteases? 4. If you wanted to isolate a copy of the gene that codes for a protein found in the skin, could that gene be located in liver cells? Explain your reasoning 5. What do you think will be the first step in purifying DNA from intact isolated cells? 6. What happened to the other macromolecules once the DNA was precipitated out of solution?

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Question:- Is DNA sequencing a in vivo, in vitro and/or in silico?   What product(s) is/are formed in DNA sequencing and how and where would the reaction begin? Also, what raw materials are needed?

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Question. Rewrite the following sentences after correction. (Subject: Biotechnology) The variation in the length of tandem repeat of microsatellite DNA has serious translational affects as this is due to its coding region. Correct: If one parent has sickle cell anemia and other has carrier genotype than there is 25 % chance that any offspring is carrier. Correct: Sickled WBC block the flow of blood and Calcium as they stick together and caused by frame shift mutation. Correct: The N1303K mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303. Correct: If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates Blood type B. Correct: Indirect ELISA can detect polygenic gene expression. Correct:

Please help Why did we use biodegradable nanoparticles? Please use The worksheet below and don’t copy and paste from Google thank you

Situational problem: Alkylating agents reagents are mutagenic factors because they cause methylation of guanine in the composition of DNA with the formation of methylguanine. Why are these DNA changes dangerous dangerous? What process do you think is activated in the human body to restore the normal DNA structure? Justify the answer. Provide appropriate scheme to illustrate the answer.

I only need help with question #8. Use question #7 as context for question #8.

Please help me with this question within an hour???? with a proper explaination ASAP

Do this practice question

Bio question

INSTRUCTION: = IF BOTH STATEMENT ARE TRUE = IF FIRST STATEMENT IS TRUE WHILE SECOND STATEMENT IS FALSE = IF FIRST STATEMENT IS FALSE WHILE SECOND STATEMENT IS TRUE = IF BOTH STATEMENTS ARE FALSE   STAMENT 1: The term that refers to the synthesis of DNA using the information contained in RNA is called DNA replication STAMENT 2: The number of hydrogen bonds between guanine and cytosine is 3   ANSWER:   STAMENT 1: If the %A of bacteria is 30%, then the % (G+C) of the bacteria is 40% STAMENT 2: The DNA is plectonemic because one of the strand go in 5’ to 3’ direction while the other go in the 3’ to 5’ direction   ANSWER:   STAMENT 1: DNA replication is conservative because one of the strands in the daughter DNA comes from the parent DNA STAMENT 2: In DNA replication, the strand copied into an mRNA is called the leading strand   ANSWER:

Experiment: Bradford protein assaygive the answers (4-5 lines) of review questions in the end. the answer should be logical and understandable and without plagiarism. avoid copy-pasting. PLEASE GIVE THE ANSWER OF 3rd AND 4th QUESTION. ITS COMPULSORY.Background information:The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assessing protein concentrations for gel electrophoresis. The method described below is for a 100 µl sample volume using 5 ml color reagent. It is sensitive to about 5 to 200 micrograms protein, depending on the dye quality. In assays using 5 ml color reagent prepared in lab, the sensitive range is closer to 5 to 100 µg protein. Scale down the volume for the "micro assay procedure," which uses 1 ml cuvettes. Protocols, including…

This is homework for a practice assessment. I need some assistance with these questions, so I can use them for studying material. Thank you so much! I highly appreciate it!

Please help me with this. *Also Please Note: This is ONE problem set and according to the policy, all questions should be answered. Thank you

Need help in 1 & 2 And if possible Analysis 1,2,&3

Number 2, please.

Explain Shortly. I need help   The emergence of new molecular biology techniques has allowed researchers to determine DNA sequences quickly and efficiently. A) How could knowledge of a DNA sequence be abused? B) How could knowing a DNA sequence be helpful? C) Would you ever consent to having your DNA sequenced. Explain your answer

Procedure: Refer to the Genetic Code Table below to identify the right amino acid coded. To determine the order of bases in the first column (uNA), second column (codon) and the third column is the anticodon. Consider the complementary base pair in DNA and in RNA To identify the amino acid, took at the bases in the MRNA codon, example AUG using the Genetic Code Table. Lool: for the first letter of the MRNA codon on the left side of the genstic code table (A), the second letter of the MRNA on the second letter column (U), and the third letter on the right-side column (G). AUG codes for the amino acid -methionine, Do the same with the other codons in the chart. Genetic Code Table 2nd posttion of codon UUU Phe Phenylalanine UCU Ser S Serine Phenylalanine UC C Ser S Serine UCA Ser S Serine UCe Ser S Serine CCU Pra P Proline UAU Tyr Y Tyrosine UAC Tyr Y Tyrosine UGU Cys C ysteine UGC Cys C Cysteine UUC Phe F UUA Lau UUG Leu CUU Leu CUC CUA Leucine UGA UGG Trp W Tryptophan G stop stop L…

Can you please help me on these questions. I don’t know the right answers.

Please help me solve this problem. I am really having a hard time understanding this lesson. Please help. Kindly provide all the necessary information to this problem. Thank you! Please answer numbers 1-5 determine what amino acid will be formed from the given  DNA strand below:                          3’   T   A  C   A   T   G   C   C   G   A   A   T   G   C   C   5’ Note: Prepare the partner strand of this DNA.  Discuss how will replication happen by mentioning the enzyme needed then transcribe to form mRNA.  Discuss what will happen to mRNA, then translate, mentioning the anticodon to be used. Look at the genetic code to know what amino acid will become part of the polypeptide chain.  1.  Partner DNA strand 2. the mRNA strand 3. The tRNA  4. the formed amino acids  5. the discussion of the entire procedure