BIO101A MidTerm
.docx
keyboard_arrow_up
School
National University College *
*We aren’t endorsed by this school
Course
100
Subject
Biology
Date
Jan 9, 2024
Type
docx
Pages
8
Uploaded by AdmiralAtomKoala6254
Midterm Lab Report
Investigating Cellular Respiration
Sherri Lahr
BIO100A Survey of Bioscience Lab
Professor Dr. Darji
December 4, 2023
Introduction
For this lab experiment, I was tasked with investigating cellular respiration, using simple items
found within most households. Before jumping into the lab it is important that there is an
understanding of what cellular respiration is. “Cellular respiration is a metabolic pathway that
uses glucose to produce adenosine triphosphate (ATP), an organic compound the body can use
for energy” (Tarantino, 2023). The body uses the ATP that is produced and necessary for many
different things that are needed to sustain life. The ATP is there to provide energy in ion
transport, muscle contractions, nerve impulse propagation, substrate phosphorylation, and
chemical synthesis (Dunn & Grider, 2023). Dunn and Grider go on to explain that most of the
processes within the human body take a lot of energy and relies on 100 to 150 moles of ATP per
day (2023). Essentially, cellular respiration is an extremely important factor in the cycle of life.
With cellular respiration there are two possible ways that it can occur. According to Khan
Academy, “cellular respiration can occur both aerobically (using oxygen), or anaerobically
(without oxygen)” (Cellular Respiration, n.d.). With cellular respiration, the ATP is formed with
glucose interacts with oxygen, and it also creates carbon dioxide along with water as byproducts
(Cellular Respiration, n.d.). When I heard this the first thing I thought of was breathing, since we
take in oxygen and dispel carbon dioxide. According to Khan Academy, even though these two
processes are related they are not the same as breathing is a physiological process (n.d.).
In this lab, the items needed included active dry instant or rapid rise yeast, 6
small
bottles, kitchen thermometer, balloons, marker, sugar, string, ruler, measuring cup and spoons,
kitchen pot, and string. I was lucky enough to have most of the items within my house, I had to
go to the local store to purchase yeast and balloons. I purchased a small container of Red Star
Yeast and the 6 balloons required for around $8.00. Now it was time to set up the experiment and
see what I was able to conclude.
Methods
Once I was home with all the necessary supplies, I pulled my kids into the kitchen to
have them work with me on this assignment because I thought it would be a good learning
experience. I labeled my bottles with a marker, 1 through 6, and set them on the counter next to
my stove. I started adding the necessary materials to the bottles and set up the balloons next to
them to make sure that I did not miss anything. Following the lab data sheet, I put 1 teaspoon of
sugar in bottles 1 through 3, 1/3 of a teaspoon in bottle 4, no sugar in bottle 5, and my own
variable choice of 2 teaspoons in bottle 6. I grabbed the active yeast and measured out 2
teaspoons of yeast 6 separate times and added them to the bottles. Next, I used the measuring cup
and measured out 1/4 of a cup 6 times to add them to the bottles. This was where my kiddos got
the become involved, we put caps on the bottles and I showed them how to gently rotate the
bottles to make sure the yeast, sugar, and water mixed together to make them as even as possible.
After we completed this, we put the balloons on the bottles after removing the caps. I
made sure that each balloon was on tightly along with the being as close as possible to the same
area on each bottle. I used the marker to indicate where on the bottle the mixed solution was
before moving to the next step. This allowed me to obtain the before measurements. I grabbed
my pot and added about 2 or 3 inches of water in it, in this case, only the first bottle was required
to remain at room temperature which was around 72 degrees Fahrenheit, so I tput in bottles 2
through 6 within the pan. To ensure that the bottles did not float or move, I used string and
secured the bottles within the pot. Again, ensuring that the string would not cause any dangers or
fire hazards. I determined that the temperature of the water would be a rolling boil and used my
Your preview ends here
Eager to read complete document? Join bartleby learn and gain access to the full version
- Access to all documents
- Unlimited textbook solutions
- 24/7 expert homework help
Related Questions
I need the answer quickly
arrow_forward
9:54 AM
Done
Prelab 7 - Tagged (2 of 64)
三:
Prelab 7 - Protein Catabolism
In this lab we focus on another important group of metabolism processes: the breakdown of proteins and amino acids. In
these labs we will utilize a series of media in tubes that differ in the protein for the bacteria to breakdown, and in the end
products they can detect. There are a few new vocabulary terms. Proteolytic enzymes are enzymes that breakdown proteins,
usually by first breaking apart the polypeptide into individual amino acids. Hydrolysis reaction is used to break apart the
peptide bond holding the amino acids in the polypeptide together. Hydrolysis is used to breakdown urea, a compound
produced by the body from the destruction of proteins.
Once broken down to individual amino acid, often the amine (NH2) functional group is broken off one end in a deamination
reaction, creating NH3. In a decarboxylation reaction the carboxyl group is removed, creating CO2. In a desulfuration
reaction a sulfur is…
arrow_forward
cell defense worksheet (1) [Compatibility Mode]
Qv Search in Docume
Insert
Draw
Design
Layout
References
Mailings
Review
View
Acrobat
A.
Century Gothic -
12
A A-
U - abe X,2 x²
A
A
Styles Styles
Pane
Create and
Adobe PI
Step 13: Take the "Energy and Transport Challenge!"
How many ATP did you use?
What type(s) of protein(s) were used?
Explain when each type was used.
Step 14: Take the "Osmosis Challenge!"
What is Osmosis?
What is the name of the special proieins that let water pass through?
Is this passive or active transport?
Step 15: From your Scores Sheet record:
Lab Score (% correct):
Number Correct:
Numbar Incen et
Page 2 of 3
* English (United States)
505 words
E Focus
MAR
15
MacBook Air
888
F1
F2
F3
F4
F5
F6
F7
F
@
%23
$
&
7
8.
Q
W
T
Y
arrow_forward
Please help
arrow_forward
PLEASE HELP WITH THIS ONE NOW
arrow_forward
please help me answer all questions, if not anything you can help me with... even without explanation <3 thank you so much
4. Which metabolic reaction releases energy?
5. Which reaction yields a more complex molecule and is reductive?
6. Which reaction yields a simpler molecule and is oxidative?
7. What are the products of catabolism? Which part of the cell does this pathway take place?
8. What are the products of anabolism? Which part of the cell does this pathway take place?
9. Which reaction is divergent? Why?
10. Which reaction is convergent? Why?
11. How can you relate waterfalls to a mole of glucose?
12. What are the steps in catabolism?
13. What is the common pathway of the degraded complex molecules?
arrow_forward
I need the answer quickly
arrow_forward
Can I have help with number 2 and 3?
2) 2What is the specific function of the enzyme catalase?
3) Catalase is an enzyme found in aerobic cells. What does this mean?
arrow_forward
BSC1010C
Enzymes & Cellular Regulation
Dr. Harris
4. Compare the rate of the pepsin-catalyzed reaction at pH = 1.5 with the rate of the lipase-
catalyzed reaction at pH = 1.5.
5. Compare the rate of the pepsin-catalyzed reaction at pH = 8.0 with the rate of the lipase-
catalyzed reaction at pH = 8.0.
6. Based on your understanding of protein structure, explain in detail the effect of exposing an
enzyme to a pH outside its optimal range. Include a discussion of the effect on both the
structure and function of the enzyme.
7. At what pH values is lipase likely to be denatured? Explain your answer.
arrow_forward
Please help me with this problem! Thank you!
arrow_forward
I need help with a biology question on enzyme denaturation, I need help with questions, one and two, which is regarding the graph I made, please let me know if you have any questions, thanks
Graph your observed results from Table 2. Include labeled axes, a descriptive title, and a colored key tothe data lines. Do not graph zero values.Further questions:-1. Looking at your graph, what trends occurred inTreatment 1,Treatment 2Treatment 3Treatment 4.Treatment 5Treatment 6
2. carbon dioxide was produced in treatment 6, what does that suggest about the experiment?
arrow_forward
AaBbCcDd AaBbCcDd AaBbC AaBbCcC AaB AaBbC
I Normal
1 No Spac. Heading 1 Heading 2
Title
Subtit
Paragraph
Styles
BIO 121 Section
Yeast cells use sugars to undergo the chemical reactions of cellular respiration. We will test the
ability of yeast cells to sucrose as an energy source for cellular respiration. We will examine if
various concentrations of sucrose has an effect on cellular respiration and whether the
temperature also plays a role in cellular respiration
Answer these questions based on the video posted:
Using this data table graph the data from the experiment in the video:
Amount of Foam in the Yeast Experiment|
Time
20°C/RT
30°C
40°C
50°C
60°C
O min
Ост
Ocm
Ocm
Ост
Ост
10 min
1.5cm
4cm
11cm
бст
Зст
20 min
2.5cm
9.5cm
17cm
11cm
9cm
30 min
4.5cm
13cm
18cm
10cm
11cm
Using the data presented in the video for the yeast experiment draw a line graph.
There are time points shown 0, 10, 20 and 30 minutes. Plot a line graph of
foam level(s) for each temperature versus time (Time is on…
arrow_forward
BIOMOLECULES
Please answer the questions properly.
- Multiple choice
Qyestion 1: If a cell has an adequate supply of adenine
nucleotides but requires more guanine nucleotides for
protein synthesis:
1. Glutamine-PRPP amidotransferase will not be fully
inhibited.
2. AMP will be a feedback inhibitor of the
condensation of IMP with aspartate.
3. ATP will stimulate the production of GMP from IMP.
4. ATP will inhibit nucleoside diphosphate reductase.
А. 1, 2, and 3
В. 2 and 4
С. 1, 2, 3, and 4
D. 1 and 3
arrow_forward
Transport Across the Cell Membrane Worksheet
1. For each of the following examples, state whether the solution is isotonic, hypertonic or
hypotonic and draw an arrow to indicate which way water will move.
a)
b)
100% H₂O
10% NaCl
0% NaCl
90% H₂O
83
5% salt
10% salt
arrow_forward
please help me answer these last three questions please please... even without explanation
7. What molecule does the cell use as an energy carrier? Draw its structure.
8. Why is it that this energy carrier is considered to be high energy containing phosphate?
9. Bond of this energy carrier of cells is broken through what?
arrow_forward
Read instruction and answer question
arrow_forward
BIOMOLECULES
Please answer the questions properly.
- Multiple choice
1. Which of the following best describes the transition
state of a catalyzed reaction?
A. higher in energy than that of an uncatalyzed
reaction
B. lower in energy than that of an uncatalyzed reaction
C. bound very weakly to the catalyst
D. lower energy than the reaction substrate
2. Supposed you tried to drop a piece of liver into
a hydrogen peroxide solution. You noticed that the
peroxide solution vigorously produced bubbles. Which
of the following caused the observed reaction?
A. liver and peroxide are joining together to make a
new protein
B. more peroxide is being created by the liver
C. peroxide is destroying germs in the liver
D. peroxide is being broken into water and oxygen
arrow_forward
Bio
arrow_forward
Please help, thank you so much in advance!
arrow_forward
need help with these 2 questions please, thank you!
arrow_forward
Please explain this question and how I can identify it next time!
arrow_forward
5
ASAP...
arrow_forward
urses / MEDBAS145en 21-22Spring / 2021-2022 Spring Semester / Final
Which of the following pairs depict the absolute specificity of the enzyme?
Select one:
O
O
O
O
a. All of them
b. Hexokinase - Glucose
c. Lactase - Lactose
d. Lipase - Triacylglycerol
arrow_forward
Tell me
Tage 11
5) This is the cycle of how energy is used
and created in a cell. Please tell me how this
cycle works and how energy is released,
what the full names are of ATP and ADP,
and provide me with two examples of some
work that would require the use of these
energy molecules.
English (United States)
arrow_forward
OCPS Das X
← → CO
PS Login
OCPS
+ Orders - X
Performance Matters
Question 12 of 16
Rate of reaction
Previous
Performa X
olaocps.performancematters.com/ola/ola.jsp?clientcode=flocps#
Join a Game - Quizizz K! Play Kahoot! - Enter...
V
C
Educator X 19 Exam: 01 × Plans for X
Submit Test
Figure 1. Enzyme Rate of Reaction
X = point of saturation
The diagram below shows the relationship between substrate concentration and reaction rate.
Dashboard
↑
Increasing
concentration does
not affect reaction rate
AP EL U.S. Citize X F https://sex
Performance Matte... S FLVS Login
Welcome, Asheley!
02.Cl.Biology.CRM3.2_2023
4JypvK8X X
+
OT
QMy Quizzes - Quizizz Athletic Clearance
Substrate concentration
Based on the graph, which of the following describes why continually increasing substrate does not lead to a continuous increase in reaction rate?
arrow_forward
Lesson: BIOCHEMISTRY
Parts Of Cell
Hiii tutor i hope you will help me to answer those questions. Hope u will have a heart to answer all thank youuu, this is my first time using my question subscription in biochemistry ^^
arrow_forward
Tube
#
2c
Table 3-Determination of the optimum temperature of catechol oxidase enzyme.
1st Absorbance
0 min. at Expt.
Temp.
3c
4c
Q12) After 10 min at experimental temperature was enzyme activity high or low at 75°C and 100°C?
Support your answer by looking at the difference between 2nd and 1st absorbances. (
5c
Q13) Was the enzyme denatured at 75°C and/or 100°C% Support your answer by looking at what
happens after returning enzyme to room temperature AFTER enzyme was subjected to experimental
temperatures of 20 °C,40 °C,60 °C,80 °C and 100°C. "¹
/1
Q14) What happens to the enzyme structure and function when it becomes denatured? (
Potato Expt.
extract Temp.
(mL)
2
2
2
2
room
temp
20 °C
40 °C
75 °C
Boiling
100 °C
dH₂O Catechol
(mL) (mL)
0
0
0
0
13
||
13
13
13
Start Time: 4:20
Absorbance:
0.072
Start Time: 4:25
Absorbance:
0.114
Start Time: 4:25
Absorbance:
0.132
Start Time: 4:27
Absorbance:
0.097
2nd Absorbance
after 10 min. in Expt
Temp.
Time for reading: 4:30
Absorbance:
0.128
Time…
arrow_forward
Please help me with this question within an hour urgently please?
arrow_forward
what will happen if a cell lacks the vitamins and minerals which are used in manufacturing cofactors? briefly explain your answer.
plsss help me answer this, thank you.
arrow_forward
You must fill in an answer for (4)-(10)
No need to explain the answers
JUST FILL IN THE BLANKS
THANK YOU. I NEED THE ANSWERS. PLEASE HELP ME OUT
arrow_forward
I hope you can help me in number 10 question. No need for a long explanation. Brief and concise is much appreciated. Thank you so much.
arrow_forward
SEE MORE QUESTIONS
Recommended textbooks for you
Anatomy & Physiology
Biology
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:OpenStax College
Related Questions
- I need the answer quicklyarrow_forward9:54 AM Done Prelab 7 - Tagged (2 of 64) 三: Prelab 7 - Protein Catabolism In this lab we focus on another important group of metabolism processes: the breakdown of proteins and amino acids. In these labs we will utilize a series of media in tubes that differ in the protein for the bacteria to breakdown, and in the end products they can detect. There are a few new vocabulary terms. Proteolytic enzymes are enzymes that breakdown proteins, usually by first breaking apart the polypeptide into individual amino acids. Hydrolysis reaction is used to break apart the peptide bond holding the amino acids in the polypeptide together. Hydrolysis is used to breakdown urea, a compound produced by the body from the destruction of proteins. Once broken down to individual amino acid, often the amine (NH2) functional group is broken off one end in a deamination reaction, creating NH3. In a decarboxylation reaction the carboxyl group is removed, creating CO2. In a desulfuration reaction a sulfur is…arrow_forwardcell defense worksheet (1) [Compatibility Mode] Qv Search in Docume Insert Draw Design Layout References Mailings Review View Acrobat A. Century Gothic - 12 A A- U - abe X,2 x² A A Styles Styles Pane Create and Adobe PI Step 13: Take the "Energy and Transport Challenge!" How many ATP did you use? What type(s) of protein(s) were used? Explain when each type was used. Step 14: Take the "Osmosis Challenge!" What is Osmosis? What is the name of the special proieins that let water pass through? Is this passive or active transport? Step 15: From your Scores Sheet record: Lab Score (% correct): Number Correct: Numbar Incen et Page 2 of 3 * English (United States) 505 words E Focus MAR 15 MacBook Air 888 F1 F2 F3 F4 F5 F6 F7 F @ %23 $ & 7 8. Q W T Yarrow_forward
- Please helparrow_forwardPLEASE HELP WITH THIS ONE NOWarrow_forwardplease help me answer all questions, if not anything you can help me with... even without explanation <3 thank you so much 4. Which metabolic reaction releases energy? 5. Which reaction yields a more complex molecule and is reductive? 6. Which reaction yields a simpler molecule and is oxidative? 7. What are the products of catabolism? Which part of the cell does this pathway take place? 8. What are the products of anabolism? Which part of the cell does this pathway take place? 9. Which reaction is divergent? Why? 10. Which reaction is convergent? Why? 11. How can you relate waterfalls to a mole of glucose? 12. What are the steps in catabolism? 13. What is the common pathway of the degraded complex molecules?arrow_forward
- I need the answer quicklyarrow_forwardCan I have help with number 2 and 3? 2) 2What is the specific function of the enzyme catalase? 3) Catalase is an enzyme found in aerobic cells. What does this mean?arrow_forwardBSC1010C Enzymes & Cellular Regulation Dr. Harris 4. Compare the rate of the pepsin-catalyzed reaction at pH = 1.5 with the rate of the lipase- catalyzed reaction at pH = 1.5. 5. Compare the rate of the pepsin-catalyzed reaction at pH = 8.0 with the rate of the lipase- catalyzed reaction at pH = 8.0. 6. Based on your understanding of protein structure, explain in detail the effect of exposing an enzyme to a pH outside its optimal range. Include a discussion of the effect on both the structure and function of the enzyme. 7. At what pH values is lipase likely to be denatured? Explain your answer.arrow_forward
- Please help me with this problem! Thank you!arrow_forwardI need help with a biology question on enzyme denaturation, I need help with questions, one and two, which is regarding the graph I made, please let me know if you have any questions, thanks Graph your observed results from Table 2. Include labeled axes, a descriptive title, and a colored key tothe data lines. Do not graph zero values.Further questions:-1. Looking at your graph, what trends occurred inTreatment 1,Treatment 2Treatment 3Treatment 4.Treatment 5Treatment 6 2. carbon dioxide was produced in treatment 6, what does that suggest about the experiment?arrow_forwardAaBbCcDd AaBbCcDd AaBbC AaBbCcC AaB AaBbC I Normal 1 No Spac. Heading 1 Heading 2 Title Subtit Paragraph Styles BIO 121 Section Yeast cells use sugars to undergo the chemical reactions of cellular respiration. We will test the ability of yeast cells to sucrose as an energy source for cellular respiration. We will examine if various concentrations of sucrose has an effect on cellular respiration and whether the temperature also plays a role in cellular respiration Answer these questions based on the video posted: Using this data table graph the data from the experiment in the video: Amount of Foam in the Yeast Experiment| Time 20°C/RT 30°C 40°C 50°C 60°C O min Ост Ocm Ocm Ост Ост 10 min 1.5cm 4cm 11cm бст Зст 20 min 2.5cm 9.5cm 17cm 11cm 9cm 30 min 4.5cm 13cm 18cm 10cm 11cm Using the data presented in the video for the yeast experiment draw a line graph. There are time points shown 0, 10, 20 and 30 minutes. Plot a line graph of foam level(s) for each temperature versus time (Time is on…arrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Anatomy & PhysiologyBiologyISBN:9781938168130Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark WomblePublisher:OpenStax College
Anatomy & Physiology
Biology
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:OpenStax College