BIO101A MidTerm

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Midterm Lab Report Investigating Cellular Respiration Sherri Lahr BIO100A Survey of Bioscience Lab Professor Dr. Darji December 4, 2023
Introduction For this lab experiment, I was tasked with investigating cellular respiration, using simple items found within most households. Before jumping into the lab it is important that there is an understanding of what cellular respiration is. “Cellular respiration is a metabolic pathway that uses glucose to produce adenosine triphosphate (ATP), an organic compound the body can use for energy” (Tarantino, 2023). The body uses the ATP that is produced and necessary for many different things that are needed to sustain life. The ATP is there to provide energy in ion transport, muscle contractions, nerve impulse propagation, substrate phosphorylation, and chemical synthesis (Dunn & Grider, 2023). Dunn and Grider go on to explain that most of the processes within the human body take a lot of energy and relies on 100 to 150 moles of ATP per day (2023). Essentially, cellular respiration is an extremely important factor in the cycle of life. With cellular respiration there are two possible ways that it can occur. According to Khan Academy, “cellular respiration can occur both aerobically (using oxygen), or anaerobically (without oxygen)” (Cellular Respiration, n.d.). With cellular respiration, the ATP is formed with glucose interacts with oxygen, and it also creates carbon dioxide along with water as byproducts (Cellular Respiration, n.d.). When I heard this the first thing I thought of was breathing, since we take in oxygen and dispel carbon dioxide. According to Khan Academy, even though these two processes are related they are not the same as breathing is a physiological process (n.d.). In this lab, the items needed included active dry instant or rapid rise yeast, 6 small bottles, kitchen thermometer, balloons, marker, sugar, string, ruler, measuring cup and spoons, kitchen pot, and string. I was lucky enough to have most of the items within my house, I had to go to the local store to purchase yeast and balloons. I purchased a small container of Red Star
Yeast and the 6 balloons required for around $8.00. Now it was time to set up the experiment and see what I was able to conclude. Methods Once I was home with all the necessary supplies, I pulled my kids into the kitchen to have them work with me on this assignment because I thought it would be a good learning experience. I labeled my bottles with a marker, 1 through 6, and set them on the counter next to my stove. I started adding the necessary materials to the bottles and set up the balloons next to them to make sure that I did not miss anything. Following the lab data sheet, I put 1 teaspoon of sugar in bottles 1 through 3, 1/3 of a teaspoon in bottle 4, no sugar in bottle 5, and my own variable choice of 2 teaspoons in bottle 6. I grabbed the active yeast and measured out 2 teaspoons of yeast 6 separate times and added them to the bottles. Next, I used the measuring cup and measured out 1/4 of a cup 6 times to add them to the bottles. This was where my kiddos got the become involved, we put caps on the bottles and I showed them how to gently rotate the bottles to make sure the yeast, sugar, and water mixed together to make them as even as possible. After we completed this, we put the balloons on the bottles after removing the caps. I made sure that each balloon was on tightly along with the being as close as possible to the same area on each bottle. I used the marker to indicate where on the bottle the mixed solution was before moving to the next step. This allowed me to obtain the before measurements. I grabbed my pot and added about 2 or 3 inches of water in it, in this case, only the first bottle was required to remain at room temperature which was around 72 degrees Fahrenheit, so I tput in bottles 2 through 6 within the pan. To ensure that the bottles did not float or move, I used string and secured the bottles within the pot. Again, ensuring that the string would not cause any dangers or fire hazards. I determined that the temperature of the water would be a rolling boil and used my
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