Genetics MCQs

The percentage of the agarose gel is important because a) it influences the rate of migration of the fragments b) it may cause some DNA molecules to replicate c) some DNA nucleotides may degraded due to chemical reactions with the gel  d) some DNA molecules may sink to the bottom of the well and not migrate What is the purpose of a buffer used in electrophoresis? a) Increase/decrease the current b) Conduct the current and protect the samples c) Disrupt the bands d) Stop the band migration Which of the following is an additional use of the gel electrophoresis technique? a) To express DNA b) To separate RNA c) To amplify RNA d) To amplify genes

Q12

22.Identify the single most correct answer: (a) Ames assay (test) can be used to identify carcinogens but not mutagens.  b) Conjugation in viruses can result in production of an antibiotic resistant virus.  c) Certain groups of viruses are responsible for the process of transduction in bacteria. d) Organisms in their natural environment cannot exchange genetic information.  e) c and d 24. In a use-dilution test, for microorganism X, we evaluated 3 different disinfectants T1, T2 and T3 with the following disinfectant dilutions: 1/10, 1/100, 1/1,000 and 1/10,000. T1 killed the microorganism up to the dilution of 1/100; T2 killed the microorganisms up to the dilution of 1/10,000 and T3 killed the microorganisms up to the dilution of 1/1,000. Rank the disinfectants in the order of their strength with the strongest being first, for disinfecting contaminations with microorganism Xor alternatively are they all equally effective? Choose the most correct answer.  a) T1 (the strongest), T2, T3…

What are the two ingredients in the agarose gel? a)What apparatus is used to create the wells in the gel? b) What apparatus is used to create the wells in the gel? c) To move the DNA through the gel, you must place which charge closest to the wells? d) What is an observable sign that the current is running?

Which of the following mechanisms will remove uracil and incorporate the correct base? a) Nucleotide excision repair b) Direct repair c) Base excision repair d) Mismatch repair

Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable. Which statement on the migration of DNA fragments through agarose gels is false A) Small fragments migrate faster than larger fragments because they can move faster through the agarose pores. B) Large fragments migrate faster than small fragments because they carry more negative charges. C) DNA fragments migrate towards the positive pole. D) Supercoiled DNA may migrate significantly different through the gel than linear DNA of equal size. E) The higher the agarose concentration the better the separation of smaller fragments as compared to larger fragments.

Solution B that was used during the plasmid isolation contains 0.2 M NaOH (see practical manual). Which effect does NaOH have on E. coli DNA A) It denatures genomic DNA and plasmid DNA. B) It denatures genomic DNA and but not plasmid DNA. C) It denatures plasmid DNA but not genomic DNA. D) It denatures neither genomic DNA nor plasmid DNA. E) This is unpredictable.

You followed all the steps correctly with a kit you bought last week. However, NO purified DNA came out of the miniprep (More than one answer possible). a) The plasmid was lost during culture growth b) Your tears from doing the same experiment again and again contaminated the reagents, causing them not to work c) Depurination of the DNA led to loss of genetic material d) Incomplete lysis of the cells after adding lysis buffer

Please help me?? with this question within an hour

Which of the following mechanisms will remove uracil and incorporate the correct base?a) Direct repairb) Base excision repairc) Mismatch repaird) Nucleotide excision repair

Please answer in short and ASAP .

11 Enzyme assay A) Always involves asking a substrate B) Can be very smoke or very complex C) Always involves ultraviolet light D) A-C are incorrect 12 Isolation of chromosomal (total) DNA using a spin column A) Involves a filtration step to remove many different molecules B) Lysis is an optional step C) Elution is not necessary D) Results in a very pure protein preparation 13 Isolation of chromosomal (total) DNA using a spin column A) Cells must be used using an appropriate method B) Cell lysis is required C) Elution of DNA required regardless of the cell type used in the experiment D) A-C are correct. 14 Isolation of chromosomal (total) DNA using a spin column A) Washing the spin column is optional B) DNA must be eluted from the spin column C) Does not involve centrifugation D) Only molecules that are supposed to bind to the column do so.

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Why is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.

Which of the following typically causes chromosome mutations such as inversions and translocations? A) Base modifiers B) UV light C) ionizing radiation D) base analogues E) intercalating agents

1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?

(a) Why the plasmid (containing the foreign DNA), together with the competentbacterial cells are heated at 42°C? b) What would be the next stage after heat shock? Discuss the process involved.c) How would you analyze the efficiency of the competent cells used duringtransformation?

1:19 Notepad Free Enter title... 484% SAVE UNDO UV radiation damages DNA by causing... a) frame-shift mutations b) base substitutions c) bonds between neighboring Thymine bases "thymine dimers" d) unzipping of the DNA molecue

12th, 11:59 PM Selectable markers allow Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a) a plasmid to replicate b) you to tell if the plasmid is present in a cell c) you to tell if the you have inserted a piece of DNA into a plasmid d) none of the above a. 1o C. dl

26. In some cases involying bacteriophages, the viral DNA will insert itself into the bacterial DNA. Which of the following statements does not correctly describe this type of insertion? A) The inserted viral DNA is referred to as a prophage. B) The inserted viral DNA can leave the bacterial DNA. C) The inserted viral DNA is a part of the lysogenic cycle of replication. D) The inserted viral DNA can never leave the bacterial DNA and enter the lytic cycle.

1) Which technique is best suited to determining which genes are activated in a bacterium during infection while causing disease in a person. a) SDS-page b) microarray analysis c) RFLP analysis d) clone library analysis   2)Which of the following is not an application of PCR? a) Determine if two people are related. b) Identify a bacterial pathogen in a patient sample. c) Determine the gene sequence of the gene that codes for a bacterial enterotoxin. d) These are all applications of PCR.

17. Which pair of scientists discovered the structure of the DNA double helix and helped to make recombinant DNA technology possible? 18. In this experiment, which of the following DNA molecules carries the gene for kanamycin resistance? A) the TN903 transposon B) the pUC plasmid 19. In this experiment, which of the following DNA molecules carries the gene for ampicillin resistance? A) the TN903 transposon B) the pUC plasmid 20. In this experiment, both the transposon DNA and the plasmid DNA are cut with which of the following restriction enzymes? А) НаеШ В) ЕcoRI C) SacI D) PvuII 21. The restriction enzyme used to cut the DNA generates: A) blunt ends B) sticky ends 22. In this experiment, what is required to join the transposon fragment with the linearized plasmid? A) the enzyme DNA ligase B) a buffer solution containing ATP C) an incubator set within the temperature range of 4-22°C D) all of the above 23. What is the purpose of treating the linearized plasmid with the enzyme alkaline…

B. Answer the following questions briefly. 1. How do we know that complementary base pairs in DNA really exist? 2. Compare and contrast replication, transcription and translation based on template used, direction of synthesis, protein/enzyme requirements during initiation, elongation and termination and products. CRITERIA of Replication Transcription Translation comparison 1. Template used 2. direction of synthesis 3. protein enzyme requirements a. Initiation b. Elongation c. Termination 4. Products 3. How do we know that expression of the information encoded in DNA involves an RNA intermediate?

Explain Shortly. I need help   The emergence of new molecular biology techniques has allowed researchers to determine DNA sequences quickly and efficiently. A) How could knowledge of a DNA sequence be abused? B) How could knowing a DNA sequence be helpful? C) Would you ever consent to having your DNA sequenced. Explain your answer

the nucleotide excision repair system can undo damaging caused by UV radiation? a) cataracts in lens  of the eye b) protein-protein cross-links c) thymine dimers d) alternative splicing errors  e) apurinic sites

Primer needed to initiate DNA replication is A) DNA segment B) RNA segment C) DNA-RNA hybrid D) Nucleotide

I need explanation for the why the answer is correct? And why would the other options wrong

Hello, i understand the question, but i only need to know which option is right, no need for explanation. thanks in advance.

40) What external factor (aka, damaging agent) gives rise to the following DNA mutations? Write and DRAW the structure of these external damaging agents. A) interstrand crosslinks CH2 HN NH H2N NH2 DNA DNA B) 3-methyladenine 3-meA NH2 CH3 C) 1, N2-etheno-dG N- dR 1,N2-E-dGuo

Which of the following statements is false? a) the bacterial chromosome is usually circular b) the bacterial chromosome has a single origin of replication c) the bacterial chromosome consists of a single molecule of DNA d) plasmids are small DNA molecules that occur in bacteria but are not essential - for normal function. e) Most bacterial genomes consist of fewer than 1,000 genes

a) what are restriction enzymes? b) What is the main function of restriction enzymes in nature? c) Compare and contrast the these enzymes in nature and in scientific research.

Please answer all of these questions in details Answer all of the following questions a) Define the following terms: biotechnology, Plasmid,Genetic engineering and Transformation b) List two types of medical products that can be produced using DNA tech- nology c) Relate bioethics to the continued development of genetic engineering techniques. d) Describe two major goals of the Human Genome Project. e) Distinguish between proteomics and genomics. f) Outline the different applications of biotechnology g) List some techniques of genetic engineering. h) Delineate the transgenic organisms and their significance. i) Explain the procedure for the following PCR, Gel electrophpresis and Gene therapy j) Describe human genome project and its main goals. k) Discuss the drastic environmental impacts of oil spills and how the mi- crobes like bacteria can help mitigate the problem. l) Give an account of significance of transgenic organisms in food industry. m) Describe the uses of DNA finger…

Which of the following is not a DNA repair mechanism? a) binding-protein excision repair b) nucleotide excision repair c) mismatch repair d) base excision repair

DNA unwinding is done bya) Ligaseb) Helicasec) Topoisomerased) Hexonuclease

8) Capillary electrophoresis involves a) Fragmentation of the DNA strands b) High resolution separation of fluorescently labelled DNA fragments c) Fluorescent activation of the DNA d) None of the above 9) Which of the following is not applicable to capillary electrophoresis? a) Multiplex PCR b) Forensic DNA profiling c) SNP genotyping d) Accurate DNA quantitation