Chapter 11

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Oct 30, 2023

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Copyright ©2019 F.A. Davis Company Chapter 11 Detection and Identification of Microorganisms
Copyright ©2019 F.A. Davis Company Objectives Identify advantages and disadvantages of using molecular-based methods as compared with traditional culture-based methods in the detection and identification of microorganisms. Explain the value of controls, particularly amplification controls, in ensuring the reliability of P C R results.
Copyright ©2019 F.A. Davis Company Objectives (continued) List the genes involved in the emergence of antimicrobial resistance that can be detected by nucleic acid amplification methods. Interpret pulse-field gel electrophoresis patterns to determine whether two isolates are related to or different from each other. Compare and contrast the molecular methods that are used to type bacterial strains in epidemiological investigations.
Copyright ©2019 F.A. Davis Company Target Microorganisms for Molecular- Based Testing Those that are difficult or time-consuming to isolate for example, Mycobacteria Hazardous organisms for example, Histoplasma, Coccidioides Those without reliable testing methods for example, HIV, hepatitis C virus (H C V) High-volume tests for example, S. pyogenes, N. gonorrhoeae, C. trachomatis
Copyright ©2019 F.A. Davis Company Applications of Molecular-Based Testing Rapid or high-throughput identification of microorganisms Detection and analysis of resistance genes Genotyping Classification Discovery of new microorganisms
Copyright ©2019 F.A. Davis Company Specimen Collection Preserve viability/nucleic acid integrity of target microorganisms Avoid contamination Appropriate time and site of collection (blood, urine, other) Use proper equipment (coagulant, wood or plastic swab shafts) Commercial collection kits are available
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