Analytical Lab 1 Paper

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Oct 30, 2023

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Robert Billings Lab Report 1 Calculations / Table of Data Calculations 1-5 1. From the 5 times the same beaker was weighed we received the following data points (30.4515g, 30.4517g, 30.4514g, 30.4519g, 30.4516g) To calculate the mean mass we will add all 5 and divide by 5. ( 30.4515 + 30.4517 + 30.4514 + 30.4519 + 30.4516)/5 = 30.4516g. The range is 5E-4 due to subtracting the smallest value from the largest value. The standard deviation is 0.00019. The %RSD is 0.00019(100)/30.4516 = 0.006% 2. 10 mL: (mass of 4 weights) (9.8973g, 9.8517g, 9.9342g, 9.8504g) (avg = 9.8834g) V (10mL) : ࠵?/࠵? ( !.#!$%& ’.(()!&/+, , !.#-’$& ’.(()!&/+, , !.!%.)& ’.(()!&/+, , !.#-(.& ’.(()!&/+, ) Avg Volume ( !.##%.& ’.(()!&/+, ) (9.867ml, 9.823ml, 9.905ml, 9.822ml) (9.855ml) Standard deviation = 0.0398 mL %RSD = (.(%!#+/(’(() !.#--+/ = 0.403% %Error = . 145࠵?࠵?(100)/10࠵?࠵? = -1.45% 5mL (mass of 4 weights) (5.0416g, 4.9393g, 4.9259g, 4.9583g) (avg = 4.966g) V(5mL) : ࠵?/࠵? ( -.(.’2& ’.(()!&/+, , ..!%!%& ’.(()!&/+, , ..!)-!& ’.(()!&/+, , ..!-#%& ’.(()!&/+, ) Avg Volume ( ..!22& ’.(()!&/+, ) (5.027ml, 4.925ml, 4.912ml, 4.944ml) (4.952ml)
Standard deviation: 0.0517mL %RSD = 0.0517࠵?࠵?(100)/4.952࠵?࠵? = 1.04% %Error = 0.48mL(100)/50mL = 0.96% 3. 1000uL: (mass of 4 weights) (0.9733g, 0.9875g, 0.9681g, 0.9830g, 0.9959g) (avg = 0.98156g) V (1000uL) : ࠵?/࠵? ( (.!$%%& ’.(()!&/+, , (.!#$-& ’.(()!&/+, , (.!2#’& ’.(()!&/+, , (.!#%(& ’.(()!&/+, , (.!!-!& ’.(()!&/+, ) Avg Volume ( (.!#’-2& ’.(()!&/+, ) = (0.9787ml) Volumes : (0.970ml, 0.985ml, 0.9653ml, 0.980ml, 0.993ml) Standard deviation = 0.0111 mL %RSD = (.(’’’+/(’(() (.!$#$&/+/ = 1.1% %Error = 21࠵?࠵?(100)/1000࠵?࠵? = -2.1% 500uL: (mass of 4 weights) (0.3993g, 0.4368g, 0.4940g, 0.4815g, 0.4448g) (avg = 0.45128g) V (500uL) : + 3 ( (.%!!%& !.##$%& ’( , (..%2#& !.##$%& ’( , (..!.(& !.##$%& ’( , (..#’-& !.##$%& ’( , ( .... #& !.##$%& ’( ) Avg Volume: ( (..-’)#& ’.(()!&/+, ) = (0.449ml) Volumes: (0.398ml, 0.4355ml, 0.4926ml, 0.480ml, 0.4435ml) Standard deviation = 0.03765 mL %RSD = (.(%$2-+/(’(() (...!&/+/ = 8.3% %Error = 51࠵?࠵?(100)/500࠵?࠵? = -10.2%
100uL: (mass of 4 weights) (0.0985g, 0.0954g, 0.0987g, 0.0961g, 0.0995g) (avg = 0.09764g) V (100uL) : ࠵?/࠵? ( (.(!#-& ’.(()!&/+, , (.(!-.& ’.(()!&/+, , (.(!#$& ’.(()!&/+, , (.(!2’& ’.(()!&/+, , (.(!!-& ’.(()!&/+, ) Avg Volume : ( (.(!$2.& ’.(()!&/+, ) = (0.0974ml) Volumes: (0.0982ml, 0.0951ml, 0.0984ml, 0.0958ml, 0.0992ml) Standard deviation = 0.00178 mL %RSD = (.((’$#+/(’(() (.(!$.&/+/ = 1.8% %Error = 2.64࠵?࠵?(100)/100࠵?࠵? = -2.64% 50uL: (mass of 4 weights) (0.0468g, 0.04644g, 0.0488g, 0.0490g, 0.0467g) (avg = 0.04754g) V (50uL) : ࠵?/࠵? ( (.(.2#& ’.(()!&/+, , (.(.2..& ’.(()!&/+, , (.(.##& ’.(()!&/+, , (.(.!(& ’.(()!&/+, , (.(.2$& ’.(()!&/+, ) Avg Volume: ( (.(.$-.& ’.(()!&/+, ) = (0.0474ml) Volumes: (0.0467ml, 0.0463ml, 0.0487ml, 0.0489ml, 0.0466ml) Standard deviation = 0.00125 mL %RSD = (.((’)-+/(’(() (.(.$.&/+/ = 2.6%
%Error = 2.6࠵?࠵?(100)/50࠵?࠵? = -5.2% 4. As expected, my error is higher than what is traditionally expected from the factory certification pipette. The following is the error expected vs the error I received respectively. (0.01mL,0.48mL) (0.02mL,0.145mL) (6uL,21uL) (6uL,51uL) (0.5uL,2.64uL) (0.25uL,2.60uL) This is clearly due to human error, to prevent the aforementioned. More practice with the instruments should be put into place as well as caution with ensuring that a new cap is used with every measurement when using the micropipette. Furthermore, ensuring that both the zeroing of the scale and the measurement of the water is taken with the glass screens closed on the balance. 5. N/A TABLE
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