Prelab 2
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Avery O’Brien
CHEM 343-76
Prelab Questions for Lab 2 (10 points)
1.
Heba and her friend Nora have the same unknown solid. Heba has progressed a little
faster with the purification process, but now she is standing around twiddling her
thumbs because no crystals are forming, even over an ice bath. Nora decides that Heba’s
problem is too much solvent. Determined not to have the same problem, Nora uses a
minimal volume of hot solvent to dissolve her compound before doing the gravity
filtration step. She begins to pour her hot solution into the filter cone of a long-stemmed
funnel and ….is that missed impurities sprouting up on the funnel walls? Why so shiny?
Explain what Nora did wrong and how to fix the problem. List 2 things she should do.
3pts
Nora used a long-stemmed funnel when she should have used a stemless funnel when
performing the gravity filtration. In order to get the crystals off the funnel walls she could use
distilled water to try and release the crystals from the side. Additionally, Nora could use a clean
microspatula to free the crystals from the walls.
2.
Neel’s unknown solid 1) dissolves in hot ethanol, 2) is essentially insoluble in hexane, and
3) is insoluble in cold water, but sparingly soluble in warm water. Outline a
recrystallization procedure. 4pt
A mixed solvent will have to be used for recrystallization. First Neel should dissolve his unknown
solid in hot ethanol. Then after letting the solution cool and begin to crystallize, cold water
should be added to encourage more crystal growth, making the solution cloudy. Then a gravity
filtration and vacuum filtration can be performed to completely isolate the crystals. Afterwards
the crystals can be washed to finish the purification.
3.
Where is the capillary tube placed for the microscale boiling point determination and
how is it orientated? What is collected in the capillary tube? 3 pts
The capillary tube is placed in the test tube for microscale boiling point determination and is
oriented so the opening of the capillary tube is pointing towards the bottom of the test tube
and is upside down. When the boiling point is met, liquid will collect in the capillary tube
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Related Questions
5
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A sample of rainwater was collected from four separate locations across theMetroplex. Each sample was measured once by two separate methods for the presence ofsulfate. Determine whether the two methods are equivalent with respect to giving thesame answer for [SO42-].
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>
1
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Hello, the priority to be answered would be page 1 (1st image). However, I can put part 2 (2nd image) on a new question/post. Just wanted to include that in this post for reference to whoever will answer it. Thank you!
arrow_forward
Table of chemicals and reagents with their physicochemical properties (name,
molecular formula, molecular weight, pKa, melting point, boiling point, etc.)
Write a short outline or a flow diagram of the procedure
List and safety issues
Read and understand the MSDS information for each chemical used in this practical
Experimental procedure
Please do not scratch the clear sides of the UV cuvettes as this will invalidate your results.
Part 1: Preparation of a calibration curve
A calibration curve should be constructed using at least five concentrations of the pure paracetamol standard
supplied in the range 3-15 ug/mL as follows:
Weigh about 150 mg of paracetamol accurately and transfer to a 200 mL volumetric flask. Add 50 mL of o.1 M
NaOH and dilute to about 100 mL with deionised water (DI). Shake and make up to volume with DI water. Dilute
10 mL of the resulting solution to 100 mL (volumetric flask) with DI water (= stock solution). Prepare standard
solutions containing 3, 6, 9, 12 and 15…
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PART 3- LABORATORY EQUIPMENT
EQUIPMENT NAME
FUNCTION
To hold test tubes.
Attaches to a retort stand to support glassware being heated.
To pour liquid from a large container into a small container.
To provide a flame during an experiment.
ones
To light the Bunsen burner.
To attach test tubes to a retort stand.
To clean substances out of a test tube.
To hold a hot crucible or flask.
To hold a hot test tube.
To place on the lab bench to prevent burning or to use with ring clamp.
To transfer small amounts of liquid.
To grow bacteria or contain small experiments.
To hold liquids.
To measure liquids.
To contain experiments (a stopper can be used to close the flask).
To hold a hot beaker.
To perform an experiment with a different substance in each well.
To hold chemicals and perform experiments in.
To scoop and transfer small amounts of substances,
To stir during experiments that involve mixing or dissolving.
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Please do it fast
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Please answser 18-19-20
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Please send me the question in 30 minutes it's very urgent plz
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Answer number 9
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Unknown
Mixture #1
Unknown
Mixture #2
Sample
caffeine
ibuprofen
aspirin
acetaminoph
en
Distance to
solvent front
14,70 cm 14,70cm
14.70cm1u.70 cm
1나,구 cm
|14.구0cm
5. 70 cm
11.90 cm
I.85cm
Distance
Spot(s) traveled 175 cm
13.75cm
11.9 5 cm 6.4 0 cm
13,75 cm
13.75 cm
38 78
1908
.8095
Calculated R:(s)
119
,9354
812 9
43 S
.9354
9354
#1:Aspirin
an d lbuprofen
Components of
Unknowns
#2: Ace taminophen, aspirin
an d ibu profen
D. Additional Exercises
1. Which of the substances tested is most polar? What parts of the structure of this substance is polar?
The Sul
2. Which of the substance tested is most non-polar? What parts of the structure of this substance in non-polar?
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..HELP W/ORG CHEM HW
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Table 2: Absorbance Values of Standards and Unknowns.
Sample
Blank
Standard Solution 1
Standard Solution 2
Standard Solution 3
Standard Solution 4
Standard Solution 5
Water Sample 1
Water Sample 2
Phosphate Concentration
(in ppm)
Y17
0 ppm
0.02 ppm
0.04 ppm
0.08 ppm
0.16 ppm
0.32 ppm
Freeman Lake
0.001
0.325
0.292
0.413
0.315
0.039
0.054
0.049
Absorbance at
2= 690 nm
Calculations:
1. Construct a phosphate standard curve in Excel by plotting concentration (in ppm) on your
x-axis and Absorbance (unitless) on your y-axis for your known solutions. Label the axes
on the graph and provide the curve with a title. Use a linear trendline to generate a best fit
line to your data. Label the graph with the equation and the R" value. Insert your labeled
graph in the space below.
X
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5. EXTRACTION
To 1 ml. of bromine water in a test tube add .5ml chloroform or carbon tetrachloride. Note
the color of the liquid layers.
Which layer is more colored?
What do you call such liquids which do not form a homogenous mixture?
Can you possibly make them homogenous?
How?
Transfer the mixture into a separatory funnel and add 2 ml. more of bromine water and
add 1 ml. more of chloroform of carbon tetrachlorides then shake to effect proper mixing. Let
out the lower layer through the stem of the funnel. Again add another 2 ml. to chloroform or
carbon tetrachloride to the aqueous solution in the funnel and shake as before.
How does the color of the chloroform or carbon tetrachloride layer compare with the first
extract?
How
do
you
explain
the
difference?
Is it possible that after several extractions the chloroform layer will become colorless?
Why?
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SERIAL DILUTION: PROBLEM SOLVING.
Set up five (5) tubes on the test tube rack and label them 1-5. Place 0.9 ml of saline to tube #1 and 0.5 ml of saline to tubes # 2-5. Add 0.1 ml of patient's serum to tube #1, mix and transfer 0.5 ml of the mixture to tube # 2. Then, mix and transfer 0.5 ml of the mixture to tube # 3. Mix and continue dilutions through tube # 5. Discard 0.5 ml from tube # 5. Finally, add 0.5 ml of the reagent to each tube # 1-5. Compute for the initial dilution of tube #1 and final dilution of tube #5. Show your computation and encircle your final answer.
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Please answer fast i give you upvote.
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. Be sure to understand what the dilutionfactor is for each sample (dilution factor = the number used to determine the actualconcentration of the stock UK after the diluted samples are analyzed) ?
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I need help on questions 2-4?
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Please don't provide handwriting solution
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4
= 5
= 6
7
= 8
F 10
= 11
E 12
F 13
In a 0.20 mM aqueous solution of trimethylacetic acid (C¸H,CO,H), what is the percentage of trimethylacetic acid that is dissociated? You can find some data
that is useful for solving this problem in the ALEKS Data resource.
Round your answer to 2 significant digits.
0%
Continue
Submit Assignment
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esc
80
DD
F2
F3
F4
F5
E7
F8
F9
F10
F11
F12
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7-10 subparts
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Subpart 4-6
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Line equation?
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please answer all w
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this is for the Extraction and Isolation of Caffeine from coffee lab
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Why is the glucose assay one of the most common analytical test performed in clinical chemistry.
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In Figure 7 above, the 3 columns represent 3 different samples. Which column(s) represent pure compound(s)?
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Blank 1 is an intensive property that is the basis of fractional distillation.
Blank 1 Add your answer
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Table #2 Worktable for BCA Assay
Standard
Water (μl)
Tube #
1
2
3
4
5
6
7
8
9
0
125
325
175
325
325
325
400
400
BSA source (μl)
300 stock
375 stock
325 stock
175 of tube #2
325 of tube #3
325 of tube #5
325 of tube #6
100 of tube #7
0
Protein (ug)
Add the volumes listed in Table 2 to each tube. Add the water first and then the
appropriate standard. Pipette each of the protein samples directly into the water, do not
dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and
contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each
of the tubes and write this in Table 2 (Protein (ug)).
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Which of the following are properties of a good recrystallization solvent? Check all correct answers.
Group of answer choices
boiling point of solvent < melting point of crystals
boiling point of solvent > melting point of crystals
Crystals are soluble in solvent at low temperature and soluble at high temperatures.
Crystals are soluble in solvent at high temperatures but insoluble or slightly soluble at low temperatures.
Solvent boiling point is about room temperature.
Impurities should be either soluble at all temperatures or insoluble at all temperatures.
An abundant amount of crystals must be recoverable from the solvent.
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please answer fast i give upvote
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1 and 2 answers?
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