Lab 5

A direct enzyme-linked immunosorbent assay (ELISA) test on a patient's sample is shown below. Development of a strong (dark) color reaction would indicate: 1 Antibody is adsorbed to well. 2 Patient sample is added; complementary antigen binds to antibody. 3 Enzyme-linked antibody specific 4 Enzyme's substrate () is added, for test antigen is added and binds and reaction produces a product that causes a visible color change (O). to antigen, forming sandwich. A low concentration of a specific antigen in the patient's sample O A high concentration of a specific enzyme in the patient's sample The patient's antibody is specific for the enzyme's substrate O A high concentration of a specific antigen in the patient's sample A high concentration of a specific antibody (against the test antigen) in the patient's sample

You can use this as your reference: https://www.youtube.com/watch?v=2J2t5FRnMtM

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What other diseases/conditions can you find an increased LAP activity? Give a brief description of each disease/condition. What other diseases/condition can you find a decreased LAP activity? Give a brief description of each disease/condition. Is the LAP Cytochemical Test 100% diagnostic for any particular disorder? Explain your answer Are there any disadvantages for this test? Briefly describe these possible

if the hplc chromatogram showed a large peak for LLL triglyceride, why would this be inappropriate for olive oil?

1. You have determined the mp of pure Urea and pure Cinnamic acid. Here is your actual data: Urea: mp 132.5C-133.0 °C Cinnamic acid: mp 132.5C-133.0 °C 2. You have determined the mp of mixtures of urea and cinnamic acid. Here is your actual data: 1:4 (20% and 80%) urea/cinnamic acid: mp 104.5-114.6°C 1:1 (50% each) urea/cinnamic acid: mp 103.0-109.4°C 4:1 (80% and 20%) urea/cinnamic acid: mp 113.5-126.9°C In the data section of your report present your data in tabular format. Use your data (mp of Urea, cinnamic acid, and mp of the three mixtures) to construct a melting point (on the Y-axis) versus composition (% composition on the X-axis) diagram, like the one on the left below, in your report. Do not include the mp of your unknown (a common student mistake)

This is an ungraded practice exam in which he gives us the answers but I would still like to know the reasoning behind

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Stearic acid: Description of bromine (red-orange) color persistance

Based on the TLC plate, what can you deduce? A salicylic acid B your synthesized aspirin Ca standard of aspirin A Aspirin is formed in high yield. O Aspirin is formed in low yield. O The aspirin formed is pure. O The aspirin formed is not pure. O The standards are pure. O The standards are not pure. O Aspirin is not formed.

E-book - Elementary Bioorgani × My Course - Elementary Bioorg X endocrine system - Google Do X Homework 1 Matter and Materia X Question 12 of 23 - Homework X assessments.macmillanlearning.com/sac/5338340#/5338340/11/1 Update : O Assignment Score: Lx Give Up? O Hint Check Answer 52.2% Resources < Question 12 of 23 Attempt 3 Carry out the given conversions from one metric unit of length to another. 83.5 Mm = km 4.75 nm = mm * TOOLS х10 !!!

Subparts 6-10

Carbachol causes contractions the isolated rat ileum to contract. The responses obtained to increasing concentrations of carbachol are tabulated below. Graph the data as log concentration versus % maximum response, using Excel or another spreadsheet program. Use the graph to estimate the EC50 value for carbachol and indicate its position on the graph. Conc (M) Exp 1 Exp 2 Exp 3 (% max response) (% max response) (% max response) 1.00E-09 3.00E-09 10 20 1.00E-08 45 35 48 3.00E-08 57 69 72 1.00E-07 74 81 91 3.00E-07 95 85 96 1.00E-06 100 100 99 3.00E-06 100 98 99

Q1: For an enzymatic assay, tumour cells are incubated at different concentrations in individual vials. Please note that the final reaction volume differs between the three setups.a) Calculate the weight (in gram/ml) of tumour cells in each vial. Show your workings.b) Calculate the glucose concentration in each vial (in mol/ml). Show your workings.  Q2: Based on Table 1, calculate the glucose concentration / 1 gram tumour cells in conditions A, B and C. Show your workings.

I don’t understand how to do part b of question 5. The other picture is my work of part a. How would changing the enzyme concentration affect Km and Vmax. The answer given to us is Vo= 0.796mM/s. I just don’t understand how to get to that answer.

Please refer to the image attached below and answer the following question. Thanks What referrals would you give to her and in what order?

Estimate (from your data) the [Protein] of your unknown based on comparing to the standards. Include the correct unit. This estimate should be an integer (no decimals).

When an organic reaction is undergoing reflux, what are some potential errors (not human or instrumental) that would cause the reflux to be incomplete?

If an association between an exposure and an association is differs significantly in strata of a third variable there is an interaction between an exposure and that third variable. O True O False

G neural crest tissue - Google Sear x A CHM 112 112-1 and 112-E1 A Presentation Session Student + 8 https://app.peardeck.com/student/twkkhzvar For quick access, place your favorites here on the favorites bar. Manage favorites now Peardeck Exercises Use #3. Nitol6) + 3 Fa(6)Z NF(1) + 3 HF(G) [1: 5,0 x10°n 0.10M Calculat K 2.0M 3.5 x 10°M Pear Deck Interactive Slide Students, draw anywhere on this slide! Do not remove this bar Slide 1/2

Help Explain one effective or ineffective aspect of this graph.

Tube Tris/CaCl, pH8 (ml) BAPNA Trypsin (µL) (µL) Final [BAPNA] (mM) 1 2.895 5.0 100 2 2.89 10.0 100 3 2.875 25.0 100 4 2.85 50.0 100 5 2.80 100 100 6 2.70 200 100 7 2.50 400 100 8 2.10 800 100

Fill out this table, involving the compounds which will be used in this lab: Acetominophen Aspirin Caffeine Compounds (Analgesics) Structure Phenacetin Salicylamide Based on these structures, which of the analgesics is the most polar, and which is the least polar? Which will travel the highest on the TLC plate when using a low-polarity solvent?

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This problem can be worked with Equations 18-6 on a calculator or with the spreadsheet in Figure 18-5. Transferrin is the iron-transport protein found in blood. It has a molecular mass of 81 000 and carries two Fe³* ions. Desferrioxamine B is a chelator used to 18-A. treat patients with iron overload (Box 11-1). It has a molecular mass of about 650 and can bind one Fe³+. Desferrioxamine can take iron from many sites within the body and is excreted (with its iron) through the kidneys. Molar absorptivities of these compounds (satu- rated with iron) at two wavelengths are given in the table. Both com- pounds are colorless (no visible absorption) in the absence of iron. e(M¯' cm¯') A(nm) Transferrin Desferrioxamine 428 3 540 2 730 470 4 170 2 290 (a) A solution of transferrin exhibits an absorbance of 0.463 at 470 nm in a 1.000-cm cell. Calculate the concentration of transferrin in mil- ligrams per milliliter and the concentration of bound iron in micro- grams per milliliter. (b) After…