allura red report
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Cleveland State University *
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Course
331
Subject
Chemistry
Date
Dec 6, 2023
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docx
Pages
3
Uploaded by DoctorWombatPerson951
Experiment Title: Determination of a dye concentration using a UV-Vis spectrophotometer
Goals:
Utilizes UV-vis spectroscopy to determine the concentration of an unknown solution of Allura red dye.
Results
:
A.
Serial Dilution
What was the concentration of your stock solution provided? 7*10^-5
Fill out the following the following table of content:
0
[Allura Red], (M
Stock Solution
Volume Prepared
(mL)
[Allura Red]
(stock)
, (M)
Volume of Stock
Pipetted (mL)
Absorbance at
= 504 nm
3.5*10^-6
10
5x10^-5
0.5
0.1327
1.4*10^-5
10
5x10^-5
2
0.4039
2.1*10^-5
10
5x10^-5
3
0.5907
2.8*10^-5
10
5x10^-5
4
0.7816
3.5*10^-5
10
5x10^-5
5
0.9093
B. Calculations:
Show the calculations of the volume of the stock solution necessary to prepare each of the stock solutions.
(1.5/30 points)
1)
3.5x10
-6
M
(3.50*10^-6 M)(10mL)=(5*10^-5 M)( _mL)
( _mL) =(3.50*10^-6 M)(10mL)/(5*10^-5 M)
=0.7mL
2)
1.4x10
-5
M
(1.40*10^-5 M)(10mL)= (5*10^-5 M)( _mL)
( _mL) =(1.40*10^-5 M)(10mL)/(5*10^-5 M)
=2.8 mL
3)
2.1x10
-5
M
(2.10*10^-5 M)(10mL)=(5*10^-5 M)( _mL)
( _mL) =(2.10*10^-5 M)(10mL)/(5*10^-5 M)
= 4.2 mL
4)
2.8x10
-5
M
(2.80*10^-5 M)(10mL)=(5*10^-5 M)( _mL)
( _mL) =(2.80*10^-5 M)(10mL)/(5*10^-5 M)
= 5.6 mL
5)
3.5x10
-5
M
(3.50*10^-5 M)(10mL)=(5*10^-5 M)( _mL)
( _mL) =(3.50*10^-5 M)(10mL)/(5*10^-5 M)
= 7.0 mL
Calibration Curve:
Create a graph of Absorbance at
(504 nm) versus [Allura Red]. Make sure to have your linear regression
passing through the origin point (0,0)
0
0
0
0
0
0
0
0
0
0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
f(x) = 26093.59 x + 0.03
Absorbance vs Allura Red
Allura Red (M)
Absorbance (nm)
Linear Regression Equation: y=26094x+0.0283
What is the Molar Extinction Coefficient for Allura Red? 26094 M
-1
cm
-1
Use the linear Regression to calculate the concentration of your unknow solution of Allura Red
Unknow Absorbance: 0.1787
Unknown [Allura Red]
= 4.6*10^--3
C.
Conjugation of Colored compounds
Please attach a copy the UV-vis spectra for your colored solution here.
Yellow: 575 nm
Your preview ends here
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