allura red report

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School

Cleveland State University *

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Course

331

Subject

Chemistry

Date

Dec 6, 2023

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docx

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3

Uploaded by DoctorWombatPerson951

Report
Experiment Title: Determination of a dye concentration using a UV-Vis spectrophotometer Goals: Utilizes UV-vis spectroscopy to determine the concentration of an unknown solution of Allura red dye. Results : A. Serial Dilution What was the concentration of your stock solution provided? 7*10^-5 Fill out the following the following table of content: 0 [Allura Red], (M Stock Solution Volume Prepared (mL) [Allura Red] (stock) , (M) Volume of Stock Pipetted (mL) Absorbance at = 504 nm 3.5*10^-6 10 5x10^-5 0.5 0.1327 1.4*10^-5 10 5x10^-5 2 0.4039 2.1*10^-5 10 5x10^-5 3 0.5907 2.8*10^-5 10 5x10^-5 4 0.7816 3.5*10^-5 10 5x10^-5 5 0.9093 B. Calculations: Show the calculations of the volume of the stock solution necessary to prepare each of the stock solutions. (1.5/30 points) 1) 3.5x10 -6 M (3.50*10^-6 M)(10mL)=(5*10^-5 M)( _mL) ( _mL) =(3.50*10^-6 M)(10mL)/(5*10^-5 M) =0.7mL 2) 1.4x10 -5 M (1.40*10^-5 M)(10mL)= (5*10^-5 M)( _mL) ( _mL) =(1.40*10^-5 M)(10mL)/(5*10^-5 M) =2.8 mL 3) 2.1x10 -5 M (2.10*10^-5 M)(10mL)=(5*10^-5 M)( _mL) ( _mL) =(2.10*10^-5 M)(10mL)/(5*10^-5 M) = 4.2 mL 4) 2.8x10 -5 M (2.80*10^-5 M)(10mL)=(5*10^-5 M)( _mL) ( _mL) =(2.80*10^-5 M)(10mL)/(5*10^-5 M) = 5.6 mL
5) 3.5x10 -5 M (3.50*10^-5 M)(10mL)=(5*10^-5 M)( _mL) ( _mL) =(3.50*10^-5 M)(10mL)/(5*10^-5 M) = 7.0 mL Calibration Curve: Create a graph of Absorbance at (504 nm) versus [Allura Red]. Make sure to have your linear regression passing through the origin point (0,0) 0 0 0 0 0 0 0 0 0 0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1 f(x) = 26093.59 x + 0.03 Absorbance vs Allura Red Allura Red (M) Absorbance (nm) Linear Regression Equation: y=26094x+0.0283 What is the Molar Extinction Coefficient for Allura Red? 26094 M -1 cm -1 Use the linear Regression to calculate the concentration of your unknow solution of Allura Red Unknow Absorbance: 0.1787 Unknown [Allura Red] = 4.6*10^--3 C. Conjugation of Colored compounds Please attach a copy the UV-vis spectra for your colored solution here. Yellow: 575 nm
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