Experiment 7 Write Up

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Vanderbilt University *

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1601

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Chemistry

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Dec 6, 2023

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pdf

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3

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Introduction As a result of the “big iron scandal of 1998” the FDA feels as if certain companies are lying about the iron contents in their products along with iron tablet manufacturers putting less iron in their tablets than what they are saying. This experiment is being conducted in order to devise a method of iron extraction and measurement in certain substances and to determine the amount of iron present in cereal and in the iron tablets. Iron extraction of the cereal was done through crushing the cereal and tumbling it multiple times and the iron tablet was put through a process of decantation and gravity filtration. Experimental Procedures Cereal Extraction: Weigh 32 grams of cereal in a 600 mL beaker on a top loading balance and transfer it to a Ziploc Bag Crush the cereal into a fine powder with any tool of choice and then pour 30 grams of it into a clean 600 mL beaker. To this crushed cereal place a Teflon magnet and 250 mL of deionized water. Place the beaker on a stirring plate and stir for 10 minutes, remove the beaker, and remove the magnet with tongs. Wash off any cereal carefully as to not lose any iron with DI water. Then place the magnet into different beaker and pour 50mL of 1.0 M HNO 3 . Warm and stir the beaker until no iron is seen on the magnet. Take the magnet out and pit it back in the cereal slurry and repeat the last 2 steps at least 3 times. After the final extraction pour the HNO 3 into a 100 mL volumetric flask and wash the magnet 3 times with 10 mL of 1.0 M HNO 3 and pour each wash into the volumetric flask. Dilute to the mark with 1.0 M HNO 3 Using a different 100 mL volumetric flask make 100 mL of 0.10 M KSCN using 1.0 M HNO 3 as the solvent. Pour this into a 250 mL beaker and pipette in 1 mL of the cereal sample. Mix thoroughly and measure the absorbance. Iron Tablet Extraction: Place a 325 mg ferrous sulfate tablet into a 25 mL Erlenmeyer flask and add 20 mL of 5 M HNO 3 . Warm the mixture on a hot plate and stir occasionally until the pill is soft enough to be crushed with a glass rod. When it’s crushed heat the mixture for 10 minutes. Afterwards, decant the liquid from the slurry into a 100 mL volumetric flask. Wash the solid 4-times with 15 mL portions of DI water and decant each wash into the volumetric flask. At the end dilute to the mark with DI water and mix thoroughly Filter a portion of the prepared solution using gravity filtration. Then, using a volumetric pipet, pipet 1 mL of this solution into a 10 mL volumetric flask. Dilute the flask to the mark with 1.0 M HNO 3 .
Using a different 100 mL volumetric flask make 100 mL of 0.10 M KSCN using 1.0 M HNO 3 as the solvent. Pour this into a 250 mL beaker and pipette in 1 mL of the iron tablet sample. Mix thoroughly and measure the absorbance. Results and Calculations Calibration Curve: Iron Tablet Absorption: 0.092 A Cereal Absorption: 0.044 A 0.044= 0.0493(x= mL of FeNO3) + 0.0117 0.892= x+ 0.0117 0.881= x 0.881 mL of FeNO3 Molar mass of FeNO3= 241.86 g/mol The density of FeNO3 is = 1.68 g/mL 1.68 x 0.881= 1.48 grams of FeNO3 1.48 g of FeNO3/ 241.86 g/mol = 0.00612 moles of FeNO3 0.00612 moles of FeNO3x (1 mol of Fe/1 mol of FeNO3) = 0.00612 moles of Fe x 55.845 = 0.342 mg of iron. The Cereal sample has 0.342 mg of iron in 1.48 g so in 30 grams of cereal there is 6.93 milligrams of Iron 0.092=0.0493x+ 0.0117 1.8661= x+0.0117 1.85 mL of FeNO3 Molar mass of FeNO3= 241.86 g/mol The density of FeNO3 is = 1.68 g/mL 1.8661 x 1.68 = 3.14 g of FeNO3 3.14 g of FeNO3/241.86 g/mol = 0.01298 moles of FeNO3 0.01298 moles of FeNO3 x (1mol of Fe/1 mol of FeNO3)= 0.01298 moles of Fe
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