Paper Chromatography Lab

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School

Goodwin College *

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Course

101

Subject

Chemistry

Date

Apr 3, 2024

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docx

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5

Uploaded by JusticePartridge3570

©2018, eScience Labs Pre-Lab Questions 1. Rank the following solutions from least polar to most polar. Rank on a scale of 1-4: 1 being the least polar and 4 being the most polar. _______ 50% ISOPROPANOL / H 2 O _______ 25% ISOPROPANOL / H 2 O _______ PURE WATER _______ 75% ISOPROPANOL / H 2 O 2. Identify the analyte, eluting solvents (mobile phases), and stationary phase used in Experiment 1. 3. Why would it be important to know which food dyes are in food?
Experiment Inventory Materials Eluting Solvents Labware (3) 50 mL Beakers 30 mL 0.5% Sodium Chloride, NaCI solution100 mL Graduated Cylinder 30 mL 0.2% Sodium Chloride, NaCI solution500 mL Beaker *30 mL Distilled Water 3 Popsicle Sticks *30 mL Isopropyl Alcohol, C 3 H 8 O 3 Capillary Tubes (packaged inside a test tube) (2) 11 x 11 cm Pieces of Chromatography Paper Ruler *6 M&Ms® (2 blue, 2 green, 2 red) *Scissors *Pencil *Computer with Internet Access *Camera / Smart Phone *Tap Water Note: You must provide the materials listed in *red. ©2018, eScience Labs Experiment Pa 1 per Chromatography EXPERIMENT 1: PAPER CHROMATOGRAPHY In this experiment, you will use paper chromatography to determine the R f value for the dyes found in candy-coated chocolate pieces. The chromatography paper acts as the stationary phase for the procedure, and a variety of mobile phases will be tested. Multiple tests with different eluting solvents must be run to determine the best eluting solvent to separate the food dyes. PROCEDURE 1. Put on your safety glasses and gloves (provided in your safety box). 2. Gather three 50 mL beakers, one for each color candy you will test. 3. Place 2 M&Ms® candies of one color into a 50 mL beaker. 4. Repeat for each color of the candy you will test. You should have two green candies in one beaker, two red candies in a second beaker, and two blue candies in the third beaker. Part 1: Preparation of the Analyte 1. Use a pipette to add 1 mL of isopropyl alcohol to each 50 mL beaker. 2. Stir with a popsicle stick for one minute (the colors may not appear to be very dark). Remove the candies. Be sure to use a clean popsicle stick each time you change beakers.
©2018, eScience Labs 3. Allow the solutions to sit and concentrate while the stationary phase is prepared.
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