520 Assessment Review Assignment

You are measuring the concentration of an unknown protein sample, and the absorbance at 595 nm (or A595) of your unknown is greater than that of the highest-concentration standard solution. It is not advisable to simply extrapolate the line of the standard curve to calculate the concentration of the unknown In this scenario, what would be the best strategy to measure the concentration of your unknown (instead of extrapolating from the standard curve)? (answer in 5 sentences)

Just part b!!!

This question has multiple parts. Work all the parts to get the most points. A solution of a dye was analyzed by spectrophotometry, and the following calibration data were collected. Dye Concentration Absorbance (A) at 475 nm 0.50 x 10 M 0.24 9. 1.5 x 10 M 0.36 2.5 x 10 M 3.5 x 10 M 4.5 x 10 M 0.44 0.59 0.70 a Construct a calibration plot, and determine the slope and intercept. Slope = y-intercept =

You measure a sample in your spectrophotometer that has an absorbance of 1.00. What is the concentration of your sample in M.

Intensity (a.u.) 4000 3352 cm¹ 3500 3000 UNKNOWN B 1710 cm¹ 2000 1668 cm¹ 2500 Wavenumber (cm¹¹) 1500 1101 cm 1000 500

Analyzing Liquids (Pepsi, Ice Tea and 50% Glucose) Interpretation: (explain the figure shown)

4. C8H5NO 12 LOD P51381145N141 0 11 HSP-40-439 4000 1 T 10 : T 9 3000 1:3 T 8 7 2000 T 6 ppm 5 4 K NAVENUMSERI- 1500 3 2 1 1000 0 DEPT-90 200 180 DEPT-135 500 COS-09-761 د سل 160 2 5 total peaks 140 ・2 peaks T 120 100 80 ppm 1 →M+₁ 4 total 60 40 20 0

Pictured is an unknown IR spectrum please include any information you can as to what the identity of the substance is,

PLEASE ANSWER ASAP

Answer questions #4 please provide and explanation. Thank you.

The maximum number of spectra that can be recorded per second by ToF-MS is limited by the time taken by the slowest ions to go from the source to the detector. If the slowest ion took 45.6 microsecond to travel, at what frequency could you record spectra if a new extraction cycle were begun each time this heaviest ion reached the detector? 30000 spectra/s 10000 spectra/s 11000 spectra/s 500 spectra/s 22000 spectra/s

You are measuring the concentration of an unknown protein sample, and the absorbance at 595 nm (or A595) of your unknown is greater than that of the highest-concentration standard solution. It is not advisable to simply extrapolate the line of the standard curve to calculate the concentration of the unknown In this scenario, what would be the best strategy to measure the concentration of your unknown (instead of extrapolating from the standard curve)?

Pre-Lab Questions- To be completed BEFORE lab as an entry slip. These questions are based on content presented in the lab procedure and in lecture. 1. Consider the electromagnetic spectrum. (Reference Site) -- Increasing Frequency (v) 10² 10% 10 10² 10% Trays 1044 10 10- 10 UV 30* 10 IR Visible spectrum 10² 10* 30 Microwave FM AM Radio waves 10° 10² 10 H 10² Increasing Wavelength (2)→ 10 Increasing Wavelength in m a. What type of radiation has the longest wavelength? 10 10² 10 voto b. What type of radiation has the highest energy? Long radio waves c. Looking at the emission line spectrum for hydrogen in Figure 1. Which colored line corresponds to the emission of the highest-energy photon?

PLEASE DO A B AND C THANK YOU SOOO MUCH!!!!!!!!

the spectra is of a drug either cannabinoid, opiate, MDMA etc, please explain the spectra and what drug could it be

Arrow-pushing Instructions NOC X 14 :CI: S + Z―H N- :Ö: H

Rel. Intensity MASS SPECTRUM 100- 80- 60- 40- 20- 0.0- 20 40 60 80 100 120 m/z The compound contains chlorine. The compound contains bromine. ○ The compound contains both bromine and chlorine. O The compound contains only carbon, hydrogen, and oxygen. ○ The compound contains only carbon and hydrogen.

Experiment name: Fluorescence Spectroscopy Q1: Discusion Q2: Purpose of experiment Q3: Introduction

I need help with the following problems from 1 to 4 i have also attached fig 7.2 for the second problem

An exploration of any one application of Spectroscopy (Absorption or Emission), Mass Spectrometry (MS) - (ANY kind of mass spec)  Pick one location/project/bit of research where the data is used and explore.  Discuss on what is measured (what instrument) and why and how.  You don't have to fully teach the instrument but spend a paragraph giving the very basics of what is measured and how.  And discuss the application you discovered - just find a place the measurement is used and explain what they do - what they measure and when it tells them.

Please analyze what functional group this IR contains. Also, please let me know what functional group these chemicals contains: - isoamyl (isopentyl) acetate, - isopentyl alcohol - acetic acid.

detector Answer Bank emission monochromator excitation monochromator light source sample cell ^ex A em It is typically a grating, prism, or filter that selects a narrow wavelength band of radiation and passes it to the sample. It holds the sample and has a defined pathlength. It is typically a photomultiplier tube that generates an electric signal when struck by photons. It is positioned 90° to the incident light and selects a narrow wavelength band of radiation to pass to

What does the absorbance of a protein at 280 nm reflect and how does that information help you determine the protein concentration of a sample if you know the sequence of your protein?

OA B. OC D. CE. Compound G is an Aldehyde Compound H is a Carboxylic acid Compound is an alcohol A Compound B is a ketone Compound is an Adehyde A Compound B is a Ketone B Compound B is an Aldehyde Compound is a Ketone Compound is an Alcohol Compound B is a Carboxylic acid

The levels of Vitamin B1 in a sample of milk was determined using the Standard Addition method and technique of fluorescence spectroscopy. 18.0 ml of the milk sample was diluted to 20.0 ml using distilled water and then the fluorescence was measured. The resulting signal was 210 units. A spike was made by taking 18.0 ml of the same milk sample, adding 1.0 ml of a 6.0 ppm Vitamin B1 standard solution, and then diluting to 20.0 ml using distilled water. The signal of the spiked milk was 540 units. Calculate the concentration of Vitamin B1 in the original milk sample (in ppm). Report to 3 decimal places.

SPECTRA FOR HOMEWORK 11, CHE 230 002 This page is not to be submitted to Gradescope. Use these spectra as you answer questions on the Homework 11 document. Spectra for Problem 1 100 %T 80 60 40 20 Relative Intensity 4000 100- 80 8 60- 40- 20- 0 20 2H 2H 3000 m/z 50.0 75.0 76.0 155.0 157.0 183.0 185.0 212.0 214.0 100.0 97.5 11.8 11.7 1686 cm 40 60 80 2000 rel, intensity 11.9 16.9 17.6 28.8 27.8 Wavenumber[cm-1] 100 120 m/z 1500 7.90 7.85 7.80 7.75 7.70 7.65 7.60 7.55 1588 cm² 20 20 ₂ مسلسل..............للمسلسل ppm 140 160 180 200 220 2H 1000 3.12 3.00 2.95 2.90 3H 500 400 A. Propose a molecular formula for this compound B. Propuse. a structure selected 1.25 1.20 1.15 201

e. Using 'H NMR data of both your product and the starting material (p-toluic acid), describe hov supports the formation of 4-bromomethylbenzoic acid. Hint: use chemical shift and integration response.

Identify the molecules and why you think it is the molecule.

6. CH₁00 11 HSP-04-4492 LOD D 10 4000 9 5000 8 T 5 7 T 6 T 2005 5 ppm VES 4 2 2 T 3 1309 1 T 2 1 1000 0 580 200 180 160 140 CDS-06-431 DEPT-90 DEPT-135 2 422 peaks 120 100 pom 80 60 40 20 0

Can someone help me interpret this data to be put on my research paper? This is the absorbance spectrum fo Sulfamethoxazole (sulfonamide antibiotic). Thank you so much!

Background 1234|5| C12H22O11 + O₂ CO₂ + H₂O Mg(OH)2 + H₂SO4 MgSO4 + H₂O NaOH + CuSO4 -Na2SO4 + Cu(OH)2 CH2 + Oz = H2O + CO2 Fe + 0₂ Fe₂O3 Mg3(PO4)2 + H₂ Mg + H3PO4 NH4NO3-N₂O + H₂O Cl₂ + KBr KCI + Br₂ ck to add speaker notes % 5 A 6 D 7 Ö * 8 ( 16 17 | )