Lab 4_TLC Report

show the complete solution and write answers in 3 significant figures. DYE CALCULATED Rf Tartrazine (yellow #5)           ? Sunset yellow (yellow #6)            ? Allura red (red #40)            ? Brilliant blue (blue #1)            ?   M&M's color COMPOSITION Orange          ? Green          ? Blue          ? Brown          ?       CHROMATOGRAM BEFORE: (image 1) CHROMATOGRAM AFTER: (image 2)

According to the following TLC, do you see any evidence on the TLC that product was lost? a Yes, TLC shows the product is also in the mother liquor (filtrate). b Yes, TLC shows product in the crude sample. c No, TLC is not quantitative. d No, there is no evidence on the TLC that the product was lost

analyte concentration(C)(mg/ml) injection volume (ul) elution time (time) peak DAD signal(mAU) caffeine 1 1 4.67 302.85 aspartame 5 1 7.53 15.83 benzoic acid 1 1 8.14 89.98 saccharin 1 1 1.91 84.86 mixture(add everything above with 1:1:1:1 ratio)   1 4.47 69.58 How to get the concentration of the mixture in this case?

If 60 mL of a combination gel of hydrocortisone acetate, 1% w/w, and pramoxine, 1% w/w, is mixed with 12.5 mL of a gel containing hydrocortisone acetate, 2.5% w/w, and pramoxine, 1% w/w, calculate the percentage strength of each of the two drugs in the mixture. Answer: 1% w/w pramoxine 1.26% w/w hydrocortisone   Solution of the given answer is needed. Thank you so much and God bless. Happy new year

Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent. What could be the possible errors why student 3 had results that are quite far from that of students A and B?

Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1 Hexanes:Acetone Amount Used 3.962 g 0.143 g 9.50 mL 9.50 mL 11.00 mL Additional Observations (Color, etc.) BIU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BIU X₂ X² →

Given the following chromatogra What molecules in the mixture were separated successfully in Chromatogram A? In Chromatogram B? From the given data, what are the differences between the two chromatograms?

Use the calibration curve or linear regression equation to determine the concentration of FD&C blue dye No. 1 in Kool-aid. Food Coloring is 0.026 M in FD&C blue dye No. 1 7.5 x 10 -5 M is 29 mL -> 1000 mL

Please correct answer and don't use hand rating

how to get uncertainty?

Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).

Question:  Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC.  -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.

Question 3 (b) Given the chromatogram shown below, answer the following questions: (b) Calculate the adjusted retention time for compound/peak 3 (in minutes). 3. 10 0. 5 8. 10 Time (min) HO OH Benzoic acid Phenol Toluene Benzene Detector Signal (mAU) 20

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10:36 1 ull ? Search Expert Q&A Done Sample G.C. : Simple Distillation Fraction 3 1500- 1000- 500- Time (min) AM Simple- F3 Sample GC of products obtained from Simple distillation of solution that is 40% ethyl acetate and 60% propyl acetate. calculate the following for each sample gas chromatograph. a) retention time for each peak (label peaks) b) area of each peak c) %composition

1. The concentration of NaOH from the best three of your trials. 2. The average concentration of 3 of your trials (only use 3 trials) 3. The standard deviation of your 3 trials [If you have a calculator with stats on it, you can use that/bring it to class, but for the lab report you will have to write out your calculations.] 4. Your PPT.

Need answer step by step

50 Percentage Red No. 40 Soln 100% 80% 60% 40% Table 4.1: Dilutions of Red No. 40 & Absorbance Volume of Red No. 40 Soln (mL) 10me 8mL опи 4mL Percentage Red No. 40 Soln 100% 80% 60% 40% 20% Volume of DI Water (mL) OML 2mL LIML M UML 8ML Table 4.2: Dilutions of Red No. 40 & Absorbance Concentration Red No. 40 2. Report the wavelength with the maximum absorbance of Red No. 40 dye. Absorbance 20% 2ML Part A: Making a Calibration Curve for Beer's Law 1. Calculate the concentrations of each of your diluted Red No. 40 solutions and add these to Table 4.2. Show at least one sample calculation in the space below. 0.251 0.192 Total Volume 0.181 0·119 0.060 (mL) 10 mL 10 mL 10 mL 10 mL 10 mL

QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.