Lab 4_TLC Report

show the complete solution and write answers in 3 significant figures. DYE CALCULATED Rf Tartrazine (yellow #5)           ? Sunset yellow (yellow #6)            ? Allura red (red #40)            ? Brilliant blue (blue #1)            ?   M&M's color COMPOSITION Orange          ? Green          ? Blue          ? Brown          ?       CHROMATOGRAM BEFORE: (image 1) CHROMATOGRAM AFTER: (image 2)

SERIAL DILUTION: PROBLEM SOLVING.   Set up five (5) tubes on the test tube rack and label them 1-5. Place 0.9 ml of saline to tube #1 and 0.5 ml of saline to tubes # 2-5. Add 0.1 ml of patient's serum to tube #1, mix and transfer 0.5 ml of the mixture to tube # 2. Then, mix and transfer 0.5 ml of the mixture to tube # 3. Mix and continue dilutions through tube # 5. Discard 0.5 ml from tube # 5. Finally, add 0.5 ml of the reagent to each tube # 1-5. Compute for the initial dilution of tube #1 and final dilution of tube #5. Show your computation and encircle your final answer.

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Based on the given chromatograph, answer the following questions:1) Which of the diet sodas have the highest concentration of caffeine? Which standard best reflects this concentration?2) Which of the diet sodas have the lowest concentration of aspartame? Which standard best reflects this concentration?3) What is the approximate retention time of benzoate?

According to the following TLC, do you see any evidence on the TLC that product was lost? a Yes, TLC shows the product is also in the mother liquor (filtrate). b Yes, TLC shows product in the crude sample. c No, TLC is not quantitative. d No, there is no evidence on the TLC that the product was lost

If 60 mL of a combination gel of hydrocortisone acetate, 1% w/w, and pramoxine, 1% w/w, is mixed with 12.5 mL of a gel containing hydrocortisone acetate, 2.5% w/w, and pramoxine, 1% w/w, calculate the percentage strength of each of the two drugs in the mixture. Answer: 1% w/w pramoxine 1.26% w/w hydrocortisone   Solution of the given answer is needed. Thank you so much and God bless. Happy new year

Three students did a chromatography experiment, where Rf = distance of solute / distance of solvent. What could be the possible errors why student 3 had results that are quite far from that of students A and B?

Determination of ascorbic acid in vitamin C tablets: which analytical approach is better; volumetric or spectrophotometric analysis?

Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1 Hexanes:Acetone Amount Used 3.962 g 0.143 g 9.50 mL 9.50 mL 11.00 mL Additional Observations (Color, etc.) BIU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BIU X₂ X² →

Given the following chromatogra What molecules in the mixture were separated successfully in Chromatogram A? In Chromatogram B? From the given data, what are the differences between the two chromatograms?

Please correct answer and don't use hand rating

The following data are from the HPLC assay of paracetamol 500 mg tablets using the calibration curve method: - Weight of active ingredient in powdered tablets taken for analysis: 180 mg • Initial extraction volume: 200 mL - Dilution steps: 10 mL into 100 mL, then 5 mL into 100 mL - Linear equation: y = 12000x - 50 %3! - Area of chromatographic peak for sample is 4750. What is the theoretical concentration in final solution in mg/100 mL? (2 decimal places). Please do not write the units, just numerical value. Answer:

Table #2 Worktable for BCA Assay Standard Water (μl) Tube # 1 2 3 4 5 6 7 8 9 0 125 325 175 325 325 325 400 400 BSA source (μl) 300 stock 375 stock 325 stock 175 of tube #2 325 of tube #3 325 of tube #5 325 of tube #6 100 of tube #7 0 Protein (ug) Add the volumes listed in Table 2 to each tube. Add the water first and then the appropriate standard. Pipette each of the protein samples directly into the water, do not dribble on the side of the test tubes. The standard is at a concentration of 2 mg/ml and contains bovine serum albumin (BSA). Calculate the amount of BSA protein added to each of the tubes and write this in Table 2 (Protein (ug)).

Question:  Using a sample of aspirin for HPLC analysis. If the aspirin was contaminated (wet) with trace amounts of a solvent such as ethyl acetate, hexane, or water, would this contaminant be detected and appear as a peak in the HPLC chromatogram? Briefly explain based on the type of detector used in HPLC analysis and what type of compounds can be detected. My Answer: The HPLC analysis detects chromophores, compounds with multiple double bonds. Since water, ethyl acetate, and hexane all lack multiple pi bonds this will not be detected in the HPLC.  -Question for you, is this complete (and correct), and should I include anything about UV detection? Is UV detection used in HPLC? I thought it was only used on TLC to show compunds that arent visable.

Question 3 (b) Given the chromatogram shown below, answer the following questions: (b) Calculate the adjusted retention time for compound/peak 3 (in minutes). 3. 10 0. 5 8. 10 Time (min) HO OH Benzoic acid Phenol Toluene Benzene Detector Signal (mAU) 20

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10:36 1 ull ? Search Expert Q&A Done Sample G.C. : Simple Distillation Fraction 3 1500- 1000- 500- Time (min) AM Simple- F3 Sample GC of products obtained from Simple distillation of solution that is 40% ethyl acetate and 60% propyl acetate. calculate the following for each sample gas chromatograph. a) retention time for each peak (label peaks) b) area of each peak c) %composition

A student developed a new dye called Dye C. It was analyzed against other dyes using paper chromatography. A solution of 1:1 ethyl acetate:hexane was used as the eluent. The resulting chromatogram is shown below. What other dye/s are present in dye C?  Which dye contains the least number of pigments?

1. The concentration of NaOH from the best three of your trials. 2. The average concentration of 3 of your trials (only use 3 trials) 3. The standard deviation of your 3 trials [If you have a calculator with stats on it, you can use that/bring it to class, but for the lab report you will have to write out your calculations.] 4. Your PPT.

two chromatograms after LC separation. Which chromatogram is better to proceed for MS analysis? What mobile phase condition/composition (LC separation) was used?

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QUESTION 5 In a chromatographic separation using a polar stationary phase and a mobile phase composed of 60% hexane and 40% ethyl acetate, two compounds, benzene (peak A) and toluene (peak B), are eluted. The width of peak A at its base is 1.2 min, the width of peak B at its base is 1.3 min, and the retention time of peak A is 5.2 min and peak B is 3.5 min. What is the resolution (Rs) between these two peaks and is it acceptable? R = 1.36. It is not an acceptable resolution. R = 0.74. It is an acceptable resolution. R = 1.36. It is an acceptable resolution. R = 0.74. It is not an acceptable resolution.

25 ml of pickled brine was taken from an apple pickled with vinegar and diluted to 500 ml, 25 ml of this was taken and acidity was determined with 0.2 N (Factor: 1.05) NaOH. In the main trials, 6.3 ml - 6.5 ml - 6.9 ml, respectively, 0.1 ml base was used in the analysis without using the sample.For example, how many ppm is its acidity?

2 how do you find the answer and what is the answer for each component using the volume of first table?

Styles Voice Text in Portuguese is not being checked. Do you (i) MISSING PROOFING TOOLS want to download proofing tools and future Sensitivity Editon Download updates? 一 4 7. 10 1 11 | 12 17. What is the main side effect of anti-epileptic drugs? a. Hypertension b. Drowsiness c. Tachycardia I. d. Insomnia e. Euphoria I believe these drugs are like the ones of the work- dihidropyridines-cont 18. What is metformin used for as first choice of treatment, and why? a. Type II diabetes, because it regulates blood glucose levels b. Hyperthyroidism, because it inhibits the formation of T3 an idirm hecause it doesn't interact with other drug 9.1 7. 8.

In the Thermo Fisher application note about wine analysis (Lesson 3), the following chromatogram was collected of nine components of wine. If peak 3 has a retention time of 3.15 minutes and a peak width of 0.070 minutes, and peak 4 has a retention time of 3.24 minutes and a peak width of 0.075 minutes, what is the resolution factor between the two peaks? [Hint: it will help to review Lesson 2 for this question.] mAU 300 200 100 0 3.22 0.62 1.04 1.24 2.4 2.6 2.8 LO 34 12 ww 3.0 5 3.2 3.4 3.6 3.8 4.0 Minutes 7 8 9 4.2 4.4 4.6 4.8 5.0 5.2