LAB £5

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School

Triton College *

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Course

150

Subject

Chemistry

Date

Jan 9, 2024

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pdf

Pages

4

Uploaded by PrivatePantherPerson394

31 Laboratory 5 Report Name(s): _____________________________________________________________________ Data Table 5.1 Copper Sulfate Known Dilutions Dilution Concentration of CuSO 4 (M) Absorbency Value of CuSO 4 Color of CuSO 4 (Scale 1-5: 1 = lightest, 5 = darkest) N/A (Distilled H 2 O) 1 in 5 1 in 10 1 in 50 1 in 100 Procedure B: Determination of Copper Sulfate Unknowns Determine the absorbency values for each one of the unknown copper sulfate solutions labeled A-F using the spectrophotometer. Record this data in Data Table 5.2 and generate a standard curve on the next page to determine the concentration by graph method. A second method for calculating an unknown’s concentration is based on using an equation called the least squares fit analysis . This can be done on a computer with a mathematical algorithm. Your professor will direct you to a computer with this spreadsheet. Data Table 5.2 Copper Sulfate Unknowns Unknown Absorbency Value Concentration by Graph Method (M) Concentration by Least Squares (M) Color of CuSO 4 (Scale 1-6: 1 = lightest, 6 = darkest) A B C D E F
32 Spectrophotometry Graph Analysis: Generate a graph using the known concentration data from Data Table 5.1. Plot the absorbency value (Y-axis) against concentration (X-axis) on Figure 5.2. Make note of the largest absorbance value of your unknowns in order to scale your axis accordingly since this graph will be used to determine the unknown concentrations from Data Table 5.2. Draw the best fit line through your known data points. While the data points should fall on a single straight line, it does not have to go through every data point plotted (Figure 5.1). Figure 5.1: Sample Standardization Graph (Generate your own graph below) Figure 5.2: Standardization Graph for Copper Sulfate
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