PhysioEx Exercise 8 Activity 4
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PhysioEx Lab Report
Exercise 8: Chemical and Physical Processes of Digestion
Activity 4: Assessing Lipase Digestion of Fat
Name: Corra clawson
Date: 10 December 2023
Session ID: session-a868a06c-726e-0a98-da38-0aa721b5a15a
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Experiment Results
Predict Question
Stop & Think Questions
Which of the following is/are true of bile?
You correctly answered:
It works by a physical process.
1
The substrate used in this simulation is
You correctly answered:
vegetable oil.
2
When fatty acids are liberated by lipase, the pH
You correctly answered:
decreases.
3
One of the products of the chemical digestion of lipids is
You correctly answered:
fatty acids.
4
Predict Question: Which tube do you think will have the highest lipase activity?
Your answer:
tube 3 (lipase, deionized water, bile salts, pH 9.0 buffer).
1
What does the pH measure?
You correctly answered:
both lipase activity and fatty acid release.
1
Why is it difficult to detect whether lipase is active in tube 5 (lipase, vegetable oil, bile salts,
pH 2.0 buffer)?
You correctly answered:
The pH is already very low, so a decrease in pH might be difficult to
detect.
2
Experiment Data
Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
Review Sheet Results
What is the product of lipase hydrolysis?
You correctly answered:
fatty acids.
1
From your results, which pH is ideal for pancreatic lipase digestion?
You correctly answered:
pH 7.0.
2
Which tube confirms that there is no lipase in bile salts or vegetable oil?
You correctly answered:
tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer).
3
Where in the body does pancreatic lipase hydrolyze triglycerides?
You correctly answered:
small intestine.
4
Explain why you can't fully test the lipase activity in tube 5 (lipase, vegetable oil, bile salts, pH
2.0 buffer).
Your answer:
Answered on review sheet.
1
Which tube had the highest lipase activity? How well did the results compare with your
prediction? Discuss possible reasons why it may or may not have matched.
Your answer:
Answered on review sheet.
2
Explain why pancreatic lipase would be active in both the mouth and the pancreas.
Your answer:
Answered on review sheet.
3
Describe the process of bile emulsification of lipids and how it improves lipase activity.
Your answer:
Answered on review sheet.
4
Your preview ends here
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Average molar bond enthalpics. (Hond)
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464
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142
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351
N-N
502
N=N
730
N=N
347
F-F
615
811
414
439
331
276
293
615
Bond
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0-0
C-0
0=0
C=0
C-C
C=C
C=C
C-H
C-F
C-CI
C-Br
C-N
C=N
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kJ. mol-1
890
390
159
418
945
155
243
192
435
565
431
368
364
225
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