PhysioEx Exercise 8 Activity 4

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Washburn University *

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Chemistry

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Jan 9, 2024

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PhysioEx Lab Report Exercise 8: Chemical and Physical Processes of Digestion Activity 4: Assessing Lipase Digestion of Fat Name: Corra clawson Date: 10 December 2023 Session ID: session-a868a06c-726e-0a98-da38-0aa721b5a15a Pre-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Experiment Results Predict Question Stop & Think Questions Which of the following is/are true of bile? You correctly answered: It works by a physical process. 1 The substrate used in this simulation is You correctly answered: vegetable oil. 2 When fatty acids are liberated by lipase, the pH You correctly answered: decreases. 3 One of the products of the chemical digestion of lipids is You correctly answered: fatty acids. 4 Predict Question: Which tube do you think will have the highest lipase activity? Your answer: tube 3 (lipase, deionized water, bile salts, pH 9.0 buffer). 1 What does the pH measure? You correctly answered: both lipase activity and fatty acid release. 1 Why is it difficult to detect whether lipase is active in tube 5 (lipase, vegetable oil, bile salts, pH 2.0 buffer)? You correctly answered: The pH is already very low, so a decrease in pH might be difficult to detect. 2
Experiment Data Post-lab Quiz Results You scored 100% by answering 4 out of 4 questions correctly. Review Sheet Results What is the product of lipase hydrolysis? You correctly answered: fatty acids. 1 From your results, which pH is ideal for pancreatic lipase digestion? You correctly answered: pH 7.0. 2 Which tube confirms that there is no lipase in bile salts or vegetable oil? You correctly answered: tube 4 (deionized water, vegetable oil, bile salts, pH 7.0 buffer). 3 Where in the body does pancreatic lipase hydrolyze triglycerides? You correctly answered: small intestine. 4 Explain why you can't fully test the lipase activity in tube 5 (lipase, vegetable oil, bile salts, pH 2.0 buffer). Your answer: Answered on review sheet. 1 Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible reasons why it may or may not have matched. Your answer: Answered on review sheet. 2 Explain why pancreatic lipase would be active in both the mouth and the pancreas. Your answer: Answered on review sheet. 3 Describe the process of bile emulsification of lipids and how it improves lipase activity. Your answer: Answered on review sheet. 4
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