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Research Study on the Interaction Among Platelets and Lymphocyte Function in Patients with Liver Disease

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Subjects
The study subject was divided into 4 groups. Group I: 21 patients with hepatic schistosomiasis (12 male and 9 females). Group II: 18 patients with HCV infection (10 male and 8 females). Group III: 23 patients with concomitant hepatic schistosomiasis and HCV infections (13 male and 10 females). Group IV: 20 healthy individuals as controls (12 male and 8 females). Full history taking including contact with water canal water were collected from the study subject
Abdominal ultrasound
Ultrasonography was conducted to all study subject to assess the hepatic physical condition including the grading of portal tract thickening in S. mansoni positive patients and the extent of liver cirrhosis.
Liver function tests
Serum levels of AST,
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HCV-RNA levels were analyzed by real time polymerase chain reaction using a commercial kit (Bioer, Technology Co., Ltd) according to the manufacturer's instructions.
Flow-cytometeric analysis
Platelets rich plasma (PRP) were separated and freshly tested for CD41, CD42, CD62P and CD63 expression using fluorescein isothiocyanate (FITC) and phycoerythrin (PE) conjugated monoclonal antibodies (mAbs) (BD Biosciences. Com, Pharmingen TM). The flow cytometry for the immune cells, EDTA treated blood was labeled with 10 µl monoclonal antibodies (mAbs) in 3 tubes. Tube 1 for T cell contained CD3-peridin chlorophyll protein (PerCP), CD4–fluorescein isothiocyanate (FITC) and CD8-phycoerythrin (PE). Tube 2 for NK cells contained CD16/CD56 PE and CD 3 FITC. Tube 3 for B cells contained CD19 FITC and CD22 PE (all Becton Dickson, San Jose, Calif). A non specific Isotype Control was used in each sample. All antibodies were of IgG1k Isotype Flowcytometer Epics ® Elite ʻʻCoulterʼʼ system was used for analysis. Results were expressed as a specific percentage of positive markers, calculated by subtracting the non specific fluorescence of the Isotype control form the specific fluorescence of the mAbs. For subtraction, the manufacturer’s software was used [15].
Statistical analysis
Statistical analysis was performed using Instat software. ANOVA test was
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