[1,6-(C-14)-2,5-(C-13)]glucose Trace the course of through glycolysis and the TCA cycle. You need only to illustrate the structures of D-glucose, labeling the atoms, and of pyruvate, labeling the origin of the atoms in glucose and the distribution of the carbon isotopes in pyruvate. After one round of the TCA cycle where will the labeled carbons be found?
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- Glucose is converted to pyruvate in glycolysis, yielding a netsynthesis of 2 ATP. In certain cells pyruvate can be reconverted to glucose during gluconeogenesis. How many ATPsare required to convert pyruvate back to glucose?The conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate is the only oxidative reaction of glycolysis. True or false?What if the phosphate group for the glycolysis reaction with enzyme glyceraldehyde 3-phosphate dehydrogenase was from ATP instead of Pi? What would the complete, balanced, net rxn of glycoslysisequation be and how does this impact the overall glycolysis rxn?
- In the first stage of glycolysis, fructose-1,6-bisphosphate iscleaved to form glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. The latter molecule can then be converted to glyceraldehyde-3-phosphate. Illustrate themechanisms whereby these reactions occurWhat is the effect of an increase in the levels of glyceraldehyde-3-phosphate (G3P) on glycolysis. Make reference to at least one relevant product/byproduct of glycolysis.The glycerol derived from lipolysis of triacylglycerols is converted into glyceraldehyde 3-phosphate, which then enters into step 6 of the glycolysis pathway. What further transformations are necessary to convert glyceraldehyde 3-phosphate into pyruvate?
- The pyruvate →lactate reaction in animals is reversible, but the pyruvate → ethanol fermentation in yeast is irreversible. Explain.Why is the isomerization of glucose-6-phosphate (G6P) to fructose-6-phosphate (F6P) an important step in glycolysis? How is the isomerization of F6P back into G6P prevented?The ΔG°’ for the aldolase reaction of glycolysis in muscle is +22.8 kJ/mol. Why does the aldolase reaction proceed in the direction of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate during glycolysis?
- If 14CO2 (radioactive carbon) were incorporated into the TCA cycle via the Pyruvate Carboxylase reaction which of the following molecules would contain radioactive carbon? a. Oxaloacetate b. Citric Acid c. Isocitrate d. Alpha-ketoglutarate e. Succinyl-CoA f .Succinate g. Fumarate h. Malate3-Phosphoglycerate is a metabolite in glycolysis. Which glycolytic enzyme generates this metabolite?Why does pyruvate either form acoa or lactate? What are the conditions?