25. The following graph illustrates the consumption of oxygen in a mitochondrial preparation. Draw, on the graph, what would happen to oxygen concentration in the mitochondria under each of the following conditions (in other words, your final graph should have 4 different lines on it, clearly labeled a, b, c, and d) A .3 dte: 0cT ON 01E.0 .2t .1 1 2 3 Time, min (a) NADH (but nothing else) is added at the time marked A (b) NADH plus ADP is added at the time marked A. (c) NADH plus ADP plus a drug that prevents ATP synthase from binding to ADP at the time marked A. (d) NADH plus ADP plus a drug that prevents ATP synthase from binding ADP at the time marked A, followed by a drug that makes the inner membrane space more basic 1 minute later. [02], mM

Question
25. The following graph illustrates the consumption of oxygen in a mitochondrial preparation.
Draw, on the graph, what would happen to oxygen concentration in the mitochondria under each
of the following conditions (in other words, your final graph should have 4 different lines on it,
clearly labeled a, b, c, and d)
A
.3
dte:
0cT ON
01E.0
.2t
.1
1 2 3
Time, min
(a) NADH (but nothing else) is added at the time marked A
(b) NADH plus ADP is added at the time marked A.
(c) NADH plus ADP plus a drug that prevents ATP synthase from binding to
ADP at the time marked A.
(d) NADH plus ADP plus a drug that prevents ATP synthase from binding
ADP at the time marked A, followed by a drug that makes the inner
membrane space more basic 1 minute later.
[02], mM

Image Transcription

25. The following graph illustrates the consumption of oxygen in a mitochondrial preparation. Draw, on the graph, what would happen to oxygen concentration in the mitochondria under each of the following conditions (in other words, your final graph should have 4 different lines on it, clearly labeled a, b, c, and d) A .3 dte: 0cT ON 01E.0 .2t .1 1 2 3 Time, min (a) NADH (but nothing else) is added at the time marked A (b) NADH plus ADP is added at the time marked A. (c) NADH plus ADP plus a drug that prevents ATP synthase from binding to ADP at the time marked A. (d) NADH plus ADP plus a drug that prevents ATP synthase from binding ADP at the time marked A, followed by a drug that makes the inner membrane space more basic 1 minute later. [02], mM

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