25. The following graph illustrates the consumption of oxygen in a mitochondrial preparation.Draw, on the graph, what would happen to oxygen concentration in the mitochondria under eachof the following conditions (in other words, your final graph should have 4 different lines on it,clearly labeled a, b, c, and d)A.3dte:0cT ON01E.0.2t.11 2 3Time, min(a) NADH (but nothing else) is added at the time marked A(b) NADH plus ADP is added at the time marked A.(c) NADH plus ADP plus a drug that prevents ATP synthase from binding toADP at the time marked A.(d) NADH plus ADP plus a drug that prevents ATP synthase from bindingADP at the time marked A, followed by a drug that makes the innermembrane space more basic 1 minute later.[02], mM

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Asked Nov 4, 2019
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25. The following graph illustrates the consumption of oxygen in a mitochondrial preparation.
Draw, on the graph, what would happen to oxygen concentration in the mitochondria under each
of the following conditions (in other words, your final graph should have 4 different lines on it,
clearly labeled a, b, c, and d)
A
.3
dte:
0cT ON
01E.0
.2t
.1
1 2 3
Time, min
(a) NADH (but nothing else) is added at the time marked A
(b) NADH plus ADP is added at the time marked A.
(c) NADH plus ADP plus a drug that prevents ATP synthase from binding to
ADP at the time marked A.
(d) NADH plus ADP plus a drug that prevents ATP synthase from binding
ADP at the time marked A, followed by a drug that makes the inner
membrane space more basic 1 minute later.
[02], mM
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25. The following graph illustrates the consumption of oxygen in a mitochondrial preparation. Draw, on the graph, what would happen to oxygen concentration in the mitochondria under each of the following conditions (in other words, your final graph should have 4 different lines on it, clearly labeled a, b, c, and d) A .3 dte: 0cT ON 01E.0 .2t .1 1 2 3 Time, min (a) NADH (but nothing else) is added at the time marked A (b) NADH plus ADP is added at the time marked A. (c) NADH plus ADP plus a drug that prevents ATP synthase from binding to ADP at the time marked A. (d) NADH plus ADP plus a drug that prevents ATP synthase from binding ADP at the time marked A, followed by a drug that makes the inner membrane space more basic 1 minute later. [02], mM

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Expert Answer

Step 1

Since we are entitled to answer first three subparts of the question, we’ll answer the part a-c as you have not mentioned the part you need help with. Please resubmit the question and specify the other parts you’d like to get answered.

Step 2

The process of ATP formation, in which electrons are transferred from NADH or FADH2 to oxygen molecule by a series of reactions containing electron carriers, is termed as oxidative phosphorylation. This process occurs inside the mitochondria of a cell, and is the major producer of ATP molecule, inside a living body.

Step 3

a) If only NADH is added to the mitochondrial system, then oxygen consumption will remain high, as NADH will give donate its electrons to the system, which would get accepted by the oxygen molecule at the end of electron transport chain.

b) As ADP, along with NADH is added to the system, then proton travels down to their concentration gradient into th...

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