The catalytic mechanism of an enzyme found in the mitochondrial matrix (pH = 7.8) depends on an active site Lys residue (pKa = 9.5) that acts as a general acid. Mutation of a neighboring residue alters the microenvironment of the Lys side chain so that its pKa shifts from 9.5 to 7.2. Would the mutation cause the reaction rate to increase or decrease? Explain your answer.
The catalytic mechanism of an enzyme found in the mitochondrial matrix (pH = 7.8) depends on an active site Lys residue (pKa = 9.5) that acts as a general acid. Mutation of a neighboring residue alters the microenvironment of the Lys side chain so that its pKa shifts from 9.5 to 7.2. Would the mutation cause the reaction rate to increase or decrease? Explain your answer.
Biology 2e
2nd Edition
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:Matthew Douglas, Jung Choi, Mary Ann Clark
Chapter7: Cellular Respiration
Section: Chapter Questions
Problem 1VCQ: Figure 7.11 Dinitrophenol (DNP) is an "uncoupler" that makes the inner mitochondrial membrane...
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The catalytic mechanism of an enzyme found in the mitochondrial matrix (pH = 7.8) depends on an active site Lys residue (pKa = 9.5) that acts as a general acid. Mutation of a neighboring residue alters the microenvironment of the Lys side chain so that its pKa shifts from 9.5 to 7.2. Would the mutation cause the reaction rate to increase or decrease? Explain your answer.
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