3. You want to insert a sequence in the lacl gene to alter its function, what restriction enzyme(s) will you use? Indicate 1 or 2 restriction enzyme(s) that you will use on the gene to show the cut. 1 aagcttctcc accagcgctt tcagctgcgc cagggtctgc tgccggtcct ccgcggcggc 61 gacaggccag gccgactggc gcacgatgag ccgggtcggc agcagctcgc ggatccgcag 121 ctgcggggcc gccatcaggg caatcagccg ctccaccgcc cttttgccca acagatcgaa 181 gtcctgcgcc accgtggtga gcggcggctg gaagtaaagg ctgtcggcgg tgtcatcgta 241 gccggtcacc gataccgcct ggctgccgct gcgattgagc tgggccagcg cgctgagcac 301 gccgagcgcc atctgatcgt ttgccaccac tatggcgctg atccgcggct gcaggtggag 361 gagctcgaaa gttttctgcc agccgctggc ggcgctccag tcgccaaaca ccgtagtaga 421 gcgggcaata ttcagactgt gcaacgcctc gcgccagctg gcgagacgca gacgggcgga 481 aaccgaactt tccggtcccg ccagcagacc aaattcgcga tgccccatct cccacaggtg 541 gcgcacgcag gccccgcagc cgtcgcggtg gtcgaagcgc acgcagcaga catcggcctc 601 cggggagaca tcgagaaaca ggcaggccat atccggattg tcttgcacca gccgctcggc 661 agtggcgctc tccagcggca gactgacgat caccccgcga atatgctggg cgcgcagctc 721 gtccagccgg gcctgcagcg cgacaaaatc ggcctgcgcc ggcatcgcta tcgccacttc 781 cagctgatgc aggctggcat ggctctttat cgcggcggcg atctgcgagg gggcgtgcag 841 cgtcacggag gcggtaatca gcccgatgga gggcgccgct ttgccggcaa gcagctgcgc 901 cgagcggtta ggcacatagt gcagcgcctg catcgcgcgg atcacctgct cgcgggtacg 961 ggctgagacc acctcaggac ggttcagcac ccggcggaga ccgtctgctg ggacacgcc 1021 gcggcgcgcg acatcctcca gggttgcggt acgacgcggc atcttttctc ctcagtaata 1081 acaatgtcat acactaacat tttatattta acacacgaca aaaaacagca cttcaccccc
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
You want to insert a sequence in the lacI gene to alter its function,what restriction enzyme(s) will you use?Indicate 1 or 2restriction enzyme(s) that you will use on the gene to show the cut
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