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- 3b)Protein sequences are usually more advantageous than DNA sequence in the comparative pairwise alignment analysis. Elaborate this statement.People who carry a theoretical genetic disorder (called B-disease) can be identified from a 2kbDNA sequence. People who carry this genetic disorder have a single nucleotide polymorphismthat results in a change of GTATTC to GGATTC, a site that only occurs once at nucleotidenumber 750 in this DNA sequence. Answer the following questions based on the informationprovided.(b) If you have carried out the molecular test (based on the information above) on a 100individual and found that 24 were healthy (BB) and 26 were carriers (bb); 1) What is the ratio of heterozygous? 2) Show how can you identify the three types from the agarose gel6. a.) Which part (sugar, phosphate, or nitrogenous base) of the four types of nucleotides differ? b.) Based on the complementary base pairing rules we know that: A(denosine) pairs with _________ , and that G(uanine) pairs with _________.
- 8. Given the following two sequences, create a pairwise alignment by hand. Do not place any gaps in the sequences to optimize alignment. Use an identity matrix and BLOSUM62 substitution matrix to create the two sets of pairwise alignments. Report the identity, identity score, similarity, and similarity score.Sequence 1: ATPL MSequence 2: ATKI MThe Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 10 9 D. In your answers, show how you came up to each result?(a) How many base pairs does this bacterium contain? (b) How many full double-helical turns does this DNA contain? (c) How long is this DNA in micrometer?A genetics student was asked to draw the chemical structureof an adenine- and thymine-containing dinucleotide derivedfrom DNA. His answer is shown below. The student made morethan six major errors. One of them is circled, numbered 1, andexplained. Find five others. Circle them, number them 2 to 6, andbriefly explain each by following the attached given.
- Q.)The Bacteria Escherichia coli DNA genome has a molecular mass of about 3.1 X 109 In your answers, show how you came up to each result? (a) How many base pairs does this bacterium contain? (b) How many full double-helical turns does this DNA contain? (c) How long is this DNA in mm?10. Analyse the following DNA sequences and identify and explain the anomalies you observe. Ifthere is no anomaly specify that as well. a.) 5’ A A T C T A G C T A A T T C G C A A A T T A A T C G 3’3’ T T A G A T C G U T T A A G C G T T U A A T T A G C 5’1.Calculate the average number of nucleotide pair per micrometer of DNA double helix using the dimensions proposed by Watson and Crick. 2. Considering the number of base pairs, compute for the actual length of the given DNA strand in micrometer. (1m = 10,000Ao) 3' C G A C T A C 5' 5' G C T G A T G 3'
- What Art the Features of the Series of -omes? Define the following terms: a. Genome b. Transcriptome c. Proteome d. Metabolome e. FluxomeChemical Mutagenesis of DNA Bases Show the nucleotide sequence changes that might arise in a dsDNA (coding strand segment GCTA) upon mutagenesis with (a) HNO2, (b) bromouracil, and (C) 2-aminopurine.3. What do you understand by “Sequencing by synthesis?” What is FRED score? Give anexample.4. Define the planted motif search problem. Answer all the part and explain all the part in details with examples.