A sample of Coomassie Blue is prepared by mixing 20 µL of stock with 280 µL of 0.1 M Tris into a round-bottom microplate. Calculate the concentration of the stock solution of Coomassie in ug/ml. The sample had a resulting A550 0.530 and the extinction coefficient for Coomassie Blue at 550 nm is 0.0168 ml/(cm"ug).
A sample of Coomassie Blue is prepared by mixing 20 µL of stock with 280 µL of 0.1 M Tris into a round-bottom microplate. Calculate the concentration of the stock solution of Coomassie in ug/ml. The sample had a resulting A550 0.530 and the extinction coefficient for Coomassie Blue at 550 nm is 0.0168 ml/(cm"ug).
Chapter15: Electrophoresis And Other Separation Methods
Section: Chapter Questions
Problem 5P
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correct answer is 530, i need to see the work to get that answer
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