A sample of Coomassie Blue is prepared by mixing 20 µL of stock with 280 µL of 0.1 M Tris into a round-bottom microplate. Calculate the concentration of the stock solution of Coomassie in ug/ml. The sample had a resulting A550 0.530 and the extinction coefficient for Coomassie Blue at 550 nm is 0.0168 ml/(cm"ug).

Appl Of Ms Excel In Analytical Chemistry
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ISBN:9781285686691
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Chapter15: Electrophoresis And Other Separation Methods
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correct answer is 530, i need to see the work to get that answer

A sample of Coomassie Blue is prepared by mixing 20 µL of stock with 280 µL
of 0.1 M Tris into a round-bottom microplate. Calculate the concentration of the
stock solution of Coomassie in ug/ml.
The sample had a resulting A550 = 0.530 and the extinction coefficient for
Coomassie Blue at 550 nm is 0.0168 ml/(cm*ug).
350
2.601
35
530
Transcribed Image Text:A sample of Coomassie Blue is prepared by mixing 20 µL of stock with 280 µL of 0.1 M Tris into a round-bottom microplate. Calculate the concentration of the stock solution of Coomassie in ug/ml. The sample had a resulting A550 = 0.530 and the extinction coefficient for Coomassie Blue at 550 nm is 0.0168 ml/(cm*ug). 350 2.601 35 530
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