According to ISO/IEC 17025: a. non-standard methods are required to be validated b. method validation or verification is not required for methods published by reputable external organisations (e.g. ISO, ASME, ASTM etc.) C. all methods shall be fully validated by the laboratory
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- A sample of steel (0.506 g) was digested in acid and the solution made up to 100 cm3 with deionised water. Aliquots (25.0 cm3) of this solution were transferred into two volumetric flasks (100 cm3) labelled A and B. Flask A was made up to volume with deionised water. To flask B was added 10.0 cm3 of a 260 ppm standard solution of Mn2+ which was then also made up to volume with deionised water. The following absorbance results were obtained: Solution A = 0.356, Solution B = 0.578. Use these results to calculate the percentage (% w/w) of manganese in the steel sample.It has been notice that adulteration of palm oil with carcinogenic dyes are on rise. Use knowledge in analytical process to systematically outline a protocol to follow to process adulterated palm oil to gain quantitative and qualitative information spectroscopically about any added toxic dye. Explain also the basis of your protocol of choice.Provide a brief explanation of one specific application on High Performance Liquid Chromatography (HPLC). Incorporate the specific application name (e.g., Detection and Quantification of Mycotoxins), provide aconcise overview of sample preparation methods, outline instrumental parameters and conditions utilized, and summarize the outcomes and findings achieved through this analytical approach.
- For Indirect Iodometric Analysis of Copper... ~0.0896g KIO3 necessary to consume 350mL of 0.1 M Na2S2O3, Na2S2O3 is stored in an amber glass bottle until ready for use. Primary Standard KIO3 has 2g of KI, 50mL of DI water, and 10 mL of 1.0M HCl is added then immediately titrated with Na2S2O3 until medium yellow or straw... then 5mL of starch indicator is added and titrated again until blue black color turns clear. Unknown CuO use 1.2G of Unknown, 20mL of HNO3 heated until sample dissolved, 25 mL of DI water added and boiled until clear light blue color, after cooling 1:1 NH3 added (~34.47 mL of NH4OH reagent) until permanent deep blue color amine complex, 2g of NH4HF2 added and swirled until dissolved, 3 g of KI is added then titrated immediately with Na2S2O3 until brown color of iodide is nearly gone (brown milk color), 2 g of KSCN and 3 mL of starch indicator is then added with titration continuing until disappearance of new blue black color. 1. Na2CO3 is often added to thiosulfate…For Indirect Iodometric Analysis of Copper... ~0.0896g KIO3 necessary to consume 350mL of 0.1 M Na2S2O3, Na2S2O3 is stored in an amber glass bottle until ready for use. Primary Standard KIO3 has 2g of KI, 50mL of DI water, and 10 mL of 1.0M HCl is added then immediately titrated with Na2S2O3 until medium yellow or straw... then 5mL of starch indicator is added and titrated again until blue black color turns clear. Unknown CuO use 1.2G of Unknown, 20mL of HNO3 heated until sample dissolved, 25 mL of DI water added and boiled until clear light blue color, after cooling 1:1 NH3 added (~34.47 mL of NH4OH reagent) until permanent deep blue color amine complex, 2g of NH4HF2 added and swirled until dissolved, 3 g of KI is added then titrated immediately with Na2S2O3 until brown color of iodide is nearly gone (brown milk color), 2 g of KSCN and 3 mL of starch indicator is then added with titration continuing until disappearance of new blue black color. 4. Why is the starch indicator solution…Module Code & Title: CHM9011M Chromatography and Sep Sciences Section 1: Purpose Clarity of Purpose ): Clearly states the purpose of the SOP. Relevance to Task : Demonstrates the importance and relevance of the procedure. Alignment with Goals ): Shows how the procedure aligns with organisational goals.
- It has been noticed that adulterationof palm oil with carcinogenic dyes are on rise. Use your knowledge in analytical process to systematically outline a protocol you will follow to process adultered palm oil to gain qualitative and quantitative information spectroscopically about any added toxic dye. Explain also the basis for your protocol of choice in detail.Procedure: analytically transfer between 0.4053g of iron(III) chloride hexahydrate, FeCl 3•6 H2O (M = 270.2957g•mol-1), and dissolve in 5.5 mL distilled, deionized water. Support the tube on a stir plate and over a 15-minute period add, using the 10mL burette, 5mL of a nominal 600mM solution of 2,4 pentandione in methanol under constant stirring. To the resulting solution, add via a 10mL burette 3mL of a nominal 450mM sodium acetate solution stirring constantly. At this point, a red crystalline solid should begin to precipitate. Transfer and support the reaction vessel in the hot water bath and once the reaction mixture has reached a stable temperature in the desired range, maintain the reaction mixture at this temperature, under constant stirring, for a 15-minute period Initial weight of iron(III) chloride hexahydrate: 0.4053g The final weight of the reaction tris(acetylacetonate)iron(III): 19.6 mg 3. What is the charge on the metal in the starting material for your synthesized…1. expleain Humidification and dehumidification mass transfer operations with suitable example and industrial application with neat sketch.
- Diprivan® (propofol) is a commonly used sedative-hypnotic agent used for sedation and anesthesia during minor as well as major surgical procedures. It is a liquid preparation administered through the IV route of drug administration. Based on what you see below, this prep is most likely a(an): Emulsion Microemulsion Suspension SolutionThe concentration of purified OXA-M290 is tested with a BCA assay. Serial dilutions of a bovine serum albumin (BSA) stock solution are prepared, then pipetted into a 96-well plate; each dilution of the BSA standard is tested in triplicate. Then, bicinchoninic acid and Cu2+ ions are added to all of the wells of the plate. After incubating the plate for 1 hour, a microplate reader is used to measure the absorbance of all of the wells in the plate at 560 nm. This generates the following data: BSA conc. (μg/mL), Replicate 1 Absorbance, Replicate 2 Absorbance, Replicate 3 Absorbance 40, 1.360, 1.403, 1.481 20, 0.750, 0.745, 0.810 10, 0.380, 0.344, 0.398 5, 0.198, 0.160, 0.183 2.5, 0.090, 0.100, 0.085 1.25, 0.038, 0.043, 0.051 0.625, 0.024, 0.028, 0.019 Prepare a calibration curve using these data. You can use Excel, R, SPSS or an equivalent graphing software. In this graph, plot absorbance (y-axis) against the concentration of the protein standard (x-axis). Calculate and plot…In Ghana, it has been noticed that adulteration of palm oil with carcinogenic dyes are on the rise. Use your knowledge in analytical processes to systematically outline a protocol you will follow to process adulterated palm oil to gain qualitative and quantitative information spectroscopically about any added toxic dye. Explain also the basis for your protocol of choice.