Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre LabThe following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp).The numbers indicate the location (in terms of the base pairs) of the restriction sites for eachenzyme, Use the maps to answer the following questionsHindll 32EcoR1 1/5541Pvul 4916Eagl 942Apal 2035Pvull 3247Smal 25405. How many fragments would be produced if this plasmid was digested with both EcoRI andEagl?a. What would be the size of each fragment?6. How many fragments would be produced if this plasmid was digested with three enzymes:Hind II, Apal & Pvu lI?a. What would be the size of each fragment?7. How many fragments would be produced if this plasmid was digested with four enzymes:Hindlll, Apol Pvul, & Pvull?a. What would be the size of each fragment?Molecular Biology Part 1-2 Post Lab V1 Short.DocxPage 4Revised 12/2/2019

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Asked Dec 9, 2019
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number 5 and 5a

Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab
The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp).
The numbers indicate the location (in terms of the base pairs) of the restriction sites for each
enzyme, Use the maps to answer the following questions
Hindll 32
EcoR1 1/5541
Pvul 4916
Eagl 942
Apal 2035
Pvull 3247
Smal 2540
5. How many fragments would be produced if this plasmid was digested with both EcoRI and
Eagl?
a. What would be the size of each fragment?
6. How many fragments would be produced if this plasmid was digested with three enzymes:
Hind II, Apal & Pvu lI?
a. What would be the size of each fragment?
7. How many fragments would be produced if this plasmid was digested with four enzymes:
Hindlll, Apol Pvul, & Pvull?
a. What would be the size of each fragment?
Molecular Biology Part 1-2 Post Lab V1 Short.Docx
Page 4
Revised 12/2/2019
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Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019

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