Biuret test suitable in the detection of proteins in food and isolated protein but with a major limitation in case of milk. Explain why?Also explain why Biuret cannot be tested for protein in urine directly? How can you perform the actual test?
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Biuret test suitable in the detection of proteins in food and isolated
protein but with a major limitation in case of milk. Explain why?Also explain why Biuret cannot be tested for protein in urine directly? How can you
perform the actual test?
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- A stock solution contains 0.2 mg/mL protein. From this stock solution, 179 uL is diluted with 1.1 mL buffer. What is the concentration of protein in this dilution?give two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.Give two advantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nm.
- Give two advantages and two disadvantages to using the biuret reaction to measure protein concentration compared to measuring the protein absorbance directly at 280 nmExplain main difference would you see when conducting the nitrous acid test with aniline vs. amino-cyclohexane? Be specific.Are Xanthoproteic and Millon Nasse tests satisfactory for use in the urinary examination for protein? Why? 2. Which test can be used to show up to what stage the hydrolysis of a protein proceeds? Why?
- Mention assays other than biuret and lowry assays that are used to detect the presence of proteins. Talk about the principle, the advantages, and the disadvantages.Proteinase K should be used as the first step of DNA purification (before applying phenol CIA mixture) to remove most proteins. Proteinase K alone cannot remove all proteins from the lysate. Explain why the Proteinase K enzyme cannot remove ALL proteins from the cell lysate.A. Explain why it is essential to digest all wild-type pQE. 1.-CRYGD. B. How would you make 50 mL o 1% weight per volume (w/v) agarose solution?
- Reversible denaturation of the proteins during salting out and precipitation.Why do we see lipid octade on renal cell carcinoma amd what are the molecular pathways driving it? Also why is there increase of alkaline phosphatese in some areas?Urea Hydrolysis If the urea slant is pink, what can you say about the pH of the medium? This pH change is due to what molecule? In the urea test, what happens to the pH of the medium when an organism breaks down urea? Consider this case 4 a. Helicobacter pylori metabolizes Urea and releases Ammonia. How would this affect the microenvironment of the stomach? 4b. Is this an important consideration in the treatment of Stomach Ulcers? Why or Why not? conculsion answer all questions thank u