(c) Discuss the process of replication of DNA. (d) Derive Michaelis-Menten equation of enzymatic reaction.
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- What is the purpose and benefit of the polymerase chain reaction?Name three intermolecular forces that stabilize the shape ofbDNA, and explain how they act.Given the following DNA sequence: 5’-ATGCGGCCAAGGTCAGAGTGACA-3’ a) If this DNA strand represents the “Sense Strand” of DNA, what would be the RNA sequence? b) If this DNA strand represents the “Antisense Strand” of DNA, what would be the RNA Sequence? c) What would be the other strand of DNA?
- Suppose a future scientist explores a distant planet and discovers a novel form of double-stranded nucleic acid. When this nucleic acid is exposed to DNA polymerases from E. coli, replication takes place continuously on both strands. What conclusion can you draw about the structure of this novel nucleic acid?Is DNA fragmentation genetic?What would happen if, in the course of replication, thetopoisomerases were unable to reattach the DNA fragments of each strand after unwinding (relaxing) theDNA molecule?
- Using 14N isotope medium for DNA replication instead of 15N, what would be observed ifDNA replication were conservative in one cycle of replication/in three cycles? How aboutdispersive replication in one cycle, in three cycles?Find the complement DNA sequences to the following DNA sequences:Sequence # 1: GATATAGTSequence # 2: GAGGTTCSequence # 3: AACTAGATSequence # 4: CCTATAAGSequence # 5: AACGTGATBriefly describe the process of DNA replication in E. coli. ?