do so, they need to first prepare 500.0 mL of 1.000 M of the Tris buffer solution of pH 7.5000. The available reagents in the lab are the following: Tris base MW = 121.14 g/mol pKa = 8.07 Tris-HCl MW = 157.56 g/mol Calculate the amounts of tris base and tris-HCl needed to prepare the buffer solution.

Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter22: Proteins
Section: Chapter Questions
Problem 22.69P
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The liquid part of the Moderna vaccine contains a Tris (tromethamine) buffer. The purpose of the buffer is to make the pH level of the vaccine near to that of the human body and also to maintain the integrity of the mRNA component.
In a laboratory scale, a biochemist was asked to study the structure of the mRNA component of the Moderna vaccine. To do so, they need to first prepare 500.0 mL of 1.000 M of the Tris buffer solution of pH 7.5000. The available reagents in the lab are the following:
Tris base MW = 121.14 g/mol pKa = 8.07
Tris-HCl MW = 157.56 g/mol

Calculate the amounts of tris base and tris-HCl needed to prepare the buffer solution.

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