Estimate the Km and Vmax in the absence and in the presence of inhibitors Determine what type of inhibitors A and B are. Explain your answer.
Estimate the Km and Vmax in the absence and in the presence of inhibitors Determine what type of inhibitors A and B are. Explain your answer.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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- Estimate the Km and Vmax in the absence and in the presence of inhibitors
- Determine what type of inhibitors A and B are. Explain your answer.
![Enzyme kinetics was analyzed in the absence of inhibitors, as well as in the presence of Inhibitor A and Inhibitor B. Plot all
of the data on one graph as Michaelis-Menten saturation curves (scatter plot with smooth lines). Then, answer the questions
below:
v (mmol/min)
no inhibitor
v (mmol/min)
inhibitor A
v (mmol/min)
inhibitor B
[S]
3
3.9
2.4
1.29
5.3
3.3
2.4
12
7.8
5
3.9
24
11
6.6
6.7
36
12
7.2
8.55
72
12.2
7.6
11.8](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F84066073-0236-4792-806f-3709c1dab065%2Fb699f31c-b44f-4d8c-a105-370bce112b62%2Fv6ixitp_processed.png&w=3840&q=75)
Transcribed Image Text:Enzyme kinetics was analyzed in the absence of inhibitors, as well as in the presence of Inhibitor A and Inhibitor B. Plot all
of the data on one graph as Michaelis-Menten saturation curves (scatter plot with smooth lines). Then, answer the questions
below:
v (mmol/min)
no inhibitor
v (mmol/min)
inhibitor A
v (mmol/min)
inhibitor B
[S]
3
3.9
2.4
1.29
5.3
3.3
2.4
12
7.8
5
3.9
24
11
6.6
6.7
36
12
7.2
8.55
72
12.2
7.6
11.8
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