for E, values, calculate AG" for oxidation of malate by malate dehydrogenase.
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The reaction is
NAD+ + Malate ---> NADH + oxaloacetate + H+
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Solved in 2 steps
- Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.Examine the ActiveModel for alcohol dehydrogenase and describe the structure and function of the catalytic zinc center.Radiolabeling with 14C-Glutamate Describe the labeling pattern that would result from the introduction into the TCA cycle of glutamate labeled at Cy with 14C.
- Referring to for E′0 values, calculate ΔG°′ for oxidation of malate by malate dehydrogenase.Outline the mechanism of the conversion of a-ketoglutarate to succinylCoA catalyzed by α-ketoglutarate dehydrogenase complex. Include all products, coenzymes, and reactions in your discussion.Alpha-ketoglutarate dehydrogenase exhibits feedback inhibition by
- Why Alpha-Ketoglutarate dehydrogenase (a-KGDH) called deficiency? What are the probable causes? Explain.For myristic acid, C 13H 27CO 2H: (a) How many molecules of acetyl CoA are formed from complete β-oxidation? (b) How many cycles of β-oxidation are needed for complete oxidation?Hypercholesterolemic individuals taking statins are sometimes advised to take supplements of coenzyme Q. Explain.
- For the following aspartase reaction in the presence of the inhibitor hydroxymethylaspartate, determine Km and whether the inhibition is competitive or noncompetitive. You have to plot thegraph on the graph paper and also by using excel.[S] V, No Inhibitor V, Inhibitor Present(molarity) (arbitrary units) (same arbitrary units) 1 x 10-4 0.026 0.0105 x 10-4 0.092 0.0401.5 x 10-3 0.136 0.0862.5 x 10-3 0.150 0.1205 x 10-3 0.165 0.142The half-reactions involved in the lactate dehydrogenase (LDH) reaction and their standard reduction potentials are (see attached)). Calculate ΔG at pH 7.0 for the LDH-catalyzed reduction of pyruvate under the following conditions: (a) [lactate]/[pyruvate] = 1 and [NAD+]/[NADH] = 1. (b) [lactate]/[pyruvate] = 160 and [NAD+]/[NADH] = 160. (c) [lactate]/[pyruvate] = 1000 and [NAD+]/[NADH] = 1000. (d) Discuss the eff ect of the concentration ratios in Parts a–c on the direction of the reaction.In the complete b-oxidation of stearic acid (18:0) How many moles of acetyl CoA are produced? How many times would the fatty acid pass through the fatty acid spiral for complete oxidation?