For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts).             Stain Cationic or Anionic Dye malachite green   crystal violet   nigrosin   safranin

Q: A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their…

A: Beer Lamberts law states that ;  A=ε CIwhere 'A' is absorbance; 'ε' is molar absorptivity…

Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain

A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…

Q: What are the processes of concentrate samples analysis in Uv-visible spectroscopy?

A: UV-visible spectroscopy is a fast and simple technique that finds the analyte concentration of a…

Q: Which of the following is true regarding aceto-orcein staining? The active dyes in this strain are…

A: Option (d) is incorrect. As because the main aim of acetic orcein stain is to study the different…

Q: If you run the Lugol's test on an unknown solution and you get a black color at the end, what does…

A: The use of Lugol's iodine reagent is used to differentiate starch and glycogen from other…

Q: Record which pole the dyes traveled to by using a (+) for positive and (-) for negative pole. Hint:…

A: Staining is a technique in which the substances observed are being made visible to the eyes. In gel…

Q: What is the purpose of the pentane extraction of spinach juice? Why wouldn’t we want to spot an…

A: Thin layer chromatography, or TLC, is an analytical method to analyse mixtures by separating the…

Q: What are the underlying physical principles of paper chromatography? What is spectrophotometry, the…

A: Analysis of different compounds quantitatively and qualitatively is an important aspect of research…

Q: Principle and procedure for Mann’s methyl blue-eosin stain

A: Methyl blue stain  Methylthioninium chloride or methyl blue is a stain use to highlight animal…

Q: Which of the following is true regarding aceto-orcein staining?

A: Aceto-orcein dye is a brownish-red and crystalline dye. It consists of a variety of phenaxazones,…

Q: The following strip of chromatography paper has been placed in a polar solvent Which pigments the…

A: In chromatographic techniques, including paper chromatography, the lighter molecules will travel the…

Q: What is Dry Flash Column Chromatography? Please explain at your own words( around 500 words).

A: Column chromatography is one of the most useful methods for the separation and purification of both…

Q: Oil has the same ? Index as glass? Why is that important?*Regarding oil immersion on a microscope

A: The question is from optical microscopy field which has an application in biology. So, I am solving…

Q: Define about sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)? Explain…

A: The process aims at the separation along with the analysis of the macromolecules that include…

Q: What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b:…

A: According to bartleby expert guidelines, when multiple sub part questions are posted we are allowed…

Q: Why must heat or a surface-active agent be used with application of the primary stain during…

A: During acid fast staining first of all heat is used to fix the bacterial smear onto the slide.…

Q: What is G-banding? O A) Most commonly used banding method B) Uses giemsa dye O C) Produces…

A: A chromosome contains the genetic material, it is present in the nucleus in condensed form.

Q: Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) 1. Why do you destain the gel?

A: There are various electrophoresis techniques used for the separation and identification of proteins.…

Q: What is the principle of Giemsa staining and  components of Giemsa stain?

A: The cells are the basic structural and fundamental unit of the living system. It consists of…

Q: Notes on Mann's methyl -blue eosin stain

A: Staining is a technique that is used in microscopy to visualize the cells and cell components in a…

Q: Molecules are tagged by antibodies in __________ microscopy. a. Confocal b. Differential…

A: Microscopy is defined as that technical field that makes use of microscopes in order to visualize…

Q: List the five components of isoelectric focusing gel list the five components of isoelectric…

A: ISOELECTRIC FOCUSING(IEF)  technique used for the separation of proteins.  PRINCIPLE :- It is a…

Q: write true if the statement if correct and change the " " word/phrase to make it correct…

A: Chromatography is biochemical separation method for organic molecules or solutes of a compound…

Q: Choose the dyes or reagents used in the Endospore stain. (More than one choices are correct)   a…

A: Simple stains- A stain that employs a single stain to color a bacteria: they are quick and easy.…

Q: What are the Types of Differential Stains? explain with an example.

A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…

Q: Why is it important that the agarose is exposed to ethidium bromide, which is either added to the…

A: Ethidium bromide is utilized during separation of fragments of DNA by agarose gel electrophoresis.…

Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…

A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…

Q: Explain the difference in the working principles of Dark-field and Light-field microscopes using pKk…

A: Microscope: It is an optical instrument that uses a lens or a combination of lenses to produce…

Q: If you have an absorbance of 2.0 mL of stock dye, what kind of a dilution is needed to get it to…

A: Biochemistry is the science of study of the internal biochemical processes of organisms. The…

Q: What are the principle and basic concepts of Simple staining

A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…

Q: five components of an isoelectric focussing gel

A: The technique that separates proteins based on their isoelectric point (pI) are called isoelectric…

Q: In extraction of albumin in egg white using 100-ml water, what does albumin look like? Also, what do…

A: In extraction of albumin in egg white and placed in water some reactions are occur..

Q: When run under nondenaturing conditions (without SDS or β-mercaptoethanol), native polyacrylamide…

A: Gel electrophoresis is the technique of separation of molecules on the basis of their size and…

Q: What are the characteristics of an acidic dye? Of a basic dye? Indicate whether methylene blue is an…

A: Dyes are colored compounds that are used as coloring agents in laboratories, textile industry, etc.…

Q: What is safe view dye for? Compare it with Ethidium Bromide.

A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…

Q: 7. Short answer. Professor Forni has hired you as a research assistant and you need to make an X-gal…

A: Questions are more than one. Please repost the other questions separately. X-gal staining is central…

Q: in paper chromatography what is the basis of pigments separate?

A: Paper chromatography is a technique in which different types of pigments can be separated from each…

Q: Write the instructions, reagents, principles and the procedures for the following.  1. Periodic acid…

A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…

Q: Assume that you have an “X" solution that you do not know its concentration. But you have other X…

A: The spectrophotometric methods is used to measure the absorbance of a sample by measuring the…

Q: define the term multicolor banding,

A: Chromosomes are long thread-like structures that carry coded genetic information in the form of DNA.…

Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?

A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…

Q: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…

A: Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X…

Q: Besides 1H NMR, FT-IR, melting point, data for refractive index, what other information could you…

A: The purity of any compound is the measure of the extent to which a given substance is pure in its…

Q: Sulfamethoxazole is a type of sulfonamide antibiotic. Its absorbance ranges from 250-300 nm in a UV…

A: Sulfamethoxazole Sulfamethoxazole is a sulfonamide based bacteriostatic antibiotic, widely used to…

Q: k there are 2 staining reagents applied in differential s

A: Differential Staining-  It is a staining process which uses more than one staining reagent.  it is…

Q: What chemicals are used for the Simple Stain from our lab experience? Check all that apply. carbolic…

A: Simple staining: * The process staining the bacterial cell with a dye  to see bacterial structure…

Q: In acid fast staining, lipoidal material of acid fast cells absorbs the methylene blue. Upon the…

A:

Q: Explain the importance of steam during the acid-fast staining procedure. Discuss the advantages of…

A: Some microbes and their structure are very difficult to be observed under the microscope . So , for…

Q: what is the full name of the TISSUSE TYPE at the end of the arrow for letter F? F1) Does this look…

A: The study of the microanatomy of cells, tissues and organs that is seen under a microscope is called…

Q: Name two fixatives used in staining?

A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…