Retention factors for three solutes separated on a C8 nonpolar stationary phase at pH = 3 are given. Eluant was a 70:30 vol/vol mixture of 50 mM citrate buffer and methanol. a)Draw the dominant chemical structure of each of the substances at pH = 3. b)The retention factor for acetophenone is 4.21, for salicylic acid is 2.97, and for nicotine is zero. Explain the different retention factors.
Retention factors for three solutes separated on a C8 nonpolar stationary phase at pH = 3 are given. Eluant was a 70:30 vol/vol mixture of 50 mM citrate buffer and methanol. a)Draw the dominant chemical structure of each of the substances at pH = 3. b)The retention factor for acetophenone is 4.21, for salicylic acid is 2.97, and for nicotine is zero. Explain the different retention factors.
Organic Chemistry: A Guided Inquiry
2nd Edition
ISBN:9780618974122
Author:Andrei Straumanis
Publisher:Andrei Straumanis
Chapter7: Cycloalkanes
Section: Chapter Questions
Problem 4E
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5) Retention factors for three solutes separated on a C8 nonpolar stationary phase at pH = 3 are given. Eluant was a 70:30 vol/vol mixture of 50 mM citrate buffer and methanol.
a)Draw the dominant chemical structure of each of the substances at pH = 3.
b)The retention factor for acetophenone is 4.21, for salicylic acid is 2.97, and for nicotine is zero. Explain the different retention factors.
Transcribed Image Text:CO,H
N.
C,H5
CH3
OH
CH3
Salicylic acid
pK, = 2.97
Acetophenone
Nicotine
pK = 3.15
pK2 = 7.85
%3D
%3D
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