Smaller particles affect HPLC column efficiency in what ways? A)increase the mass transfer term. B)diminish the pressure constraints of the mobile phase. C)reduce stationary phase retention D)enable lower slopes inthe C term E)cause higher temperature as a result of increased frictional heating.

Q: sely proportional to the column efficiency irectly proportional to the selectivity factor not…

A: B option is correct. Inversely proportional to column efficiency. Band-broadening is a general term…

Q: What other method besides conductivity can be used to determine CMC  (critical micelle…

A: Critical micelle concentration (CMC) can be determined by conductometric titration.

Q: The Bradford method described in the Background section was used to determine protein concentrations…

A: Given:

Q: The original solution was diluted by a factor of three, and then the absorbance of the diluted…

A: According to Lambert Beer law, the absorbance of a solution is directly proportional to its…

Q: 1. Discuss briefly the specifications and test parameters for system suitability for HPLC analysis.

A: Important Parameters used in HPLC analysis are as follows: 1). Retention time (tR): It is the time…

Q: Which of the following results from an in-line HPLC mobile phase filter becoming plugged with…

A: Correct option is B.

Q: If the peak width w of both Peak 1 and Peak 2 fro eon tg is 0.5 minutes, what is the resolution…

A: Answer..

Q: Which is better HPLC system Agilent 1290 Infinity II LC or Nexera Series UHPLC? Explain

A: HPLC is High Performance Liquid Chromatography UHPLC is Ultra High Performance Liquid Chromatography

Q: Dye 1 Dye 2 Run 1 Run 2 Run 3 Run 1 Run 2 Run 3 Start of Band (mL} 1.1 1.1 1.2 2.2 2.2 2.3 End of…

A: Retention Volume : It is the volume of the mobile phase which is required to elute out a particular…

Q: How can band broadening be reduced in HPLC Eddy Diffusion? A. use smaller particles for stationary…

A: HPLC is based on the principle where the sample (analyte) is distributed between a mobile phase…

Q: An open tubular column with an inner diameter of 0.25 mm had a volumetric flow rate of 0.95 min-1 .…

A:

Q: On a 125cm retention times Long column. operated at look these. are obtained. octane 1.43 Air peak…

A: Since you have asked multiple questions we will answer the first one for you. To get the remaining…

Q: Relative Standard Deviation of UV Vis, please explain.

A: A question based on RSD, which is to be accomplished.

Q: The osmosis rate in these set-ups will slow down over time. Why?

A: Osmosis is the process of diffusion (movement of particles or molecules) of water across a…

Q: The following table was written after using sieve analysis method on a paracetamol powder. Sieve…

A:

Q: Determine the relationship.                                                             In…

A: To determine the relationship of ATR-FTIR with penetration depth, sample size and reflective index…

Q: polarography 3. (a). Write down the principle of polarography and deduce Ilkovic equation for the…

A: The answers are given below.

Q: Define the following terms used in HPLC:             (a) gradient elution             (b)…

A: Given terms, (a) gradient elution (b) isocratic elution (c) reversed-phase packing

Q: A 0.05 mM solution of DCPIP was placed in a 1 cm spectrophotometer cuvette and a beam of light at…

A: We know that absorbance = log (I0/I) = e*c*l

Q: A hollow fiber membrane filtration module has a hydraulic permeability of Lp %3D 10-13 m S'Pa The…

A:

Q: Which of the following is the correct flow of the mobile phase in HPLC? Solvent degasser>Guard…

A: The mobile phase in HPLC is used to transport the sample via seperation column and there by detector…

Q: Explain the reason 4-point probe method is considered to be more accurate than 2-point probe method.

A: To find: The reason for 4-point probe method is considered to be more accurate than 2-point probe…

Q: Describe the following properties of chromatogram (including formula if applicable): Bandwidth…

A: 1.Bandwidth sets the frequency around the wavelength provided by the detector to view and report…

Q: Describe the mechanisms of strength at the peak hardness of a precipitation hardened alloy when both…

A:

Q: (A) Draw a sketch of the expected chromatrogram (peaks, broadening, relative elution times, etc.)

A: Given: A) To sketch the expected chromatogram.

Q: What was the most important parameter change that lead to the development of modern HPLC?   A.…

A: answer- What was the most important parameter change that lead to the development of modern HPLC -…

Q: What are the benefits of decreasing the column internal diameter? O Increased sample capacity O…

A: Answer - The correct option is (c)  Increased resolution  Explanation - As we lower the internal…

Q: 4. Consider the following diffusion oefficients (DM and Ds, both in cm2/min), hich one will cause…

A:

Q: Given the following data for a screen analysis, calculate the mass fraction, average particle size,…

A: Solutions- Given data- Total sample size =1721 gm.

Q: ist FOUR kinetic variables that affect column efficiency and explain how each variab

A: Band broadening  reflects the the loss of column efficiency

Q: 1. Provide two advantages and two disadvantage of flow cytometry analysis. Briefly explain each.

A:

Q: Small explanation needed. In reverse-phased separations, _______ column volume(S) of initial…

A: When two or more substances are mixed in a definite proportion, it yields the mixture. The…

Q: 16 14 12 10 4 2 10 20 30 Minutes 40 50 60 70

A: From retention time and width of two peaks,Resolution for the given chromatogram can be determined.…

Q: Two students A, B did the Flame photometric analysis by different atomization methods in which the…

A: Flame Atomic Absorption Spectroscopy (FAAS), commonly called flame spectroscopy is a branch of…

Q: A gold on iron oxide catalyst was prepared by wet impregnation and dried at 110 °C overnight. The…

A:

Q: What is compensation depth?

A:

Q: Sketch a graph of the van Deemter equation (plate height versus flow rate). What would the curve…

A: The van Deemter equation is a relationship between the height of the plate during the flow of mobile…

Q: Compare gradient and isocratic mode in HPLC.

A: Gradient and isocratic mode in HPLC are the two different modes of operating condition in HPLC.

Q: Resolution of neighboring peaks is the difference in retention time divided by the average peak…

A: Welcome to bartleby !  Introdduction :  We have to tell whether the statement is true or not .

Q: How GC retention time can be affected by the mobile phase flow rate and the temperature of the…

A:

Q: List four input transducers nd very briefly describe how they are used.

A: LIST OF FOUR INPUT TRANSDCERS

Q: Calculate the Rf value of the Blue spot on the TLC Plate below. (answer must have * 2 decimal…

A: Calculate Rf value of the blue spot on TLC.  From the figure,  Distance travelled by the solvent…

Q: In HPLC, fill in the blanks with the choices below: - chromatogram retention time void volume…

A: To fill in the blanks for HPLC:

Q: Should you increase the diameter or length of the chromatography colum for increasing throughput?

A:

Q: Estimate the concentration of nitrate in the aquarium water. Aquarium water was diluted from 1 mL to…

A: According to the mole concept, in terms of mass, the amount of substance in moles is equal to the…

Q: Given below is the block diagram of gas chromatography. Identify the unmarked component Column Gas…

A: The chromatographic method used for separating and analyzing compounds that can be vaporized without…

Q: A calibration curve was created, and the results has been established and showed in the table,…

A: Data for the Calibration curve n 1.55 1.65 1.70 1.80 ??? 1.62 Conc. 10% 15% 25% 35% 22% ???…